Gordonia phage Brandonk123
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Detailed Information for Phage Brandonk123
Discovery Information
Isolation HostGordonia terrae 3612
Found ByBrandon Kretlow, Brooke Butkowski
Year Found2016
Location FoundRiver Falls, WI United States
Finding InstitutionUniversity of Wisconsin-River Falls
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?No
Isolation Temperature29°C
GPS Coordinates44.8502 N, 92.624713 W Map
Discovery Notessoil sample collected at 2:13pm under a bridge by the Uwrf sports center with moist and sandy soil conditions. at 10mm depth in 73°F in sunny conditions 54% humidity. This phage was discovered by Brooke and then further tested by Brandon in the lab
Naming NotesBrandonk123 is Brandon's name + his birthday since he is bad at naming.
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedJan 23, 2017
Sequencing FacilityPittsburgh Bacteriophage Institute
Shotgun Sequencing MethodIllumina Sequencing
Approximate Shotgun Coverage1628
Genome length (bp)58842
Character of genome endsCircularly Permuted
GC Content67.3%
Fasta file available?Yes: Download fasta file
Characterization
ClusterDE
SubclusterDE1
Cluster Life CycleLytic
Other Cluster Members
Annotation StatusIn GenBank
Plaque Notesthe plaques are in the shape of a semihalo and are about 4-5 mm in diameter
MorphotypeSiphoviridae
Has been Phamerated?Yes
Gene List

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Brandonk123_1

Brandonk123_2
(terminase)

Brandonk123_3

Brandonk123_4

Brandonk123_5

Brandonk123_6

Brandonk123_7

Brandonk123_8

Brandonk123_9

Brandonk123_10

Brandonk123_11
(DNA methylase)

Brandonk123_12

Brandonk123_13

Brandonk123_14

Brandonk123_15
(portal protein)

Brandonk123_16

Brandonk123_17

Brandonk123_18

Brandonk123_19
(capsid maturation protease)

Brandonk123_20

Brandonk123_21

Brandonk123_22
(major capsid protein)

Brandonk123_23

Brandonk123_24

Brandonk123_25
(major tail protein)

Brandonk123_26

Brandonk123_27
(hydrolase)

Brandonk123_28
(head-to-tail connector complex protein)

Brandonk123_29

Brandonk123_30

Brandonk123_31

Brandonk123_32

Brandonk123_33

Brandonk123_34
(tape measure protein)

Brandonk123_35
(minor tail protein)

Brandonk123_36
(minor tail protein)

Brandonk123_37
(HNH endonuclease)

Brandonk123_38

Brandonk123_39
(minor tail protein)

Brandonk123_40

Brandonk123_41

Brandonk123_42
(HNH endonuclease)

Brandonk123_43
(lysin A)

Brandonk123_44
(membrane protein)

Brandonk123_45
(membrane protein)

Brandonk123_46
(membrane protein)

Brandonk123_47
(lysin B)

Brandonk123_48

Brandonk123_49

Brandonk123_50
(exonuclease)

Brandonk123_51

Brandonk123_52

Brandonk123_53
(ssDNA binding protein)

Brandonk123_54

Brandonk123_55

Brandonk123_56
(HTH DNA binding protein)

Brandonk123_57

Brandonk123_58
(HTH DNA binding protein)

Brandonk123_59

Brandonk123_60

Brandonk123_61

Brandonk123_62

Brandonk123_63

Brandonk123_64

Brandonk123_65

Brandonk123_66

Brandonk123_67

Brandonk123_68

Brandonk123_69

Brandonk123_70

Brandonk123_71

Brandonk123_72

Brandonk123_73
(resolvase)

Brandonk123_74

Brandonk123_75

Brandonk123_76

Brandonk123_77

Brandonk123_78

Brandonk123_79

Brandonk123_80

Brandonk123_81

Brandonk123_82

Brandonk123_83

Brandonk123_84

Brandonk123_85

Brandonk123_86

Brandonk123_87

Brandonk123_88
(HTH DNA binding protein)

Brandonk123_89
(DNA methylase)

Submitted Minimal DNA Master FileDownload
Publication Info
Uploaded to GenBank?Yes
GenBank AccessionMG812487
Refseq NumberNone yet
Archiving Info
Archiving status Archived
SEA Lysate Titer 2.1x10^9
Date of SEA Lysate Titering Oct 10, 2016
Pitt Freezer Box# 37
Pitt Freezer Box Grid# E3
Available Files
EM PictureDownload
GenBank File for PhameratorDownload