Mycobacterium phage Dylan
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Detailed Information for Phage Dylan
Discovery Information
Isolation HostMycobacterium smegmatis mc²155
Found ByVanisha Munsamy
Year Found2011
Location FoundDurban, South Africa
Finding InstitutionUniversity of Kwazulu-Natal
ProgramUnavailable
From enriched soil sample?Yes
Isolation TemperatureNot entered
GPS Coordinates29.8587 N, 31.0218 E Map
Discovery NotesFound in moist soil on the property of 472 Grove End Drive, Phoenix, Durban, South Africa. Soil sample taken from 2-3cm deep ground. Temperature approximately 20-25 degrees Celsius.
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedJun 29, 2012
Sequencing FacilityPittsburgh Bacteriophage Institute
Shotgun Sequencing MethodIon Torrent Sequencing
Approximate Shotgun Coverage865
Sanger Finishing Reactions30
Genome length (bp)69815
Character of genome ends3' Sticky Overhang
Overhang Length4 bp
Overhang SequenceGTGT
GC Content65.4%
Fasta file available?Yes: Download fasta file
Characterization
ClusterO
Subcluster--
Other Cluster Members
Plaque NotesPlaques approximately 0.2 mm in diameter, small and round in shape. Turbid plaques were observed.
5 phage particles were measured using a Transmission Election Microscope. The average morphology is as follows: Head width: 32.1nm, head length: 142.8nm, tail length: 233.68nm.
MorphotypeSiphoviridae
Final annotation complete?Yes
Number of Genes121
Has been Phamerated?Yes
Gene List

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Dylan_1

Dylan_2

Dylan_3

Dylan_4

Dylan_5

Dylan_6
(DNA Methylase)

Dylan_7
(DNA Methylase)

Dylan_8

Dylan_9

Dylan_10

Dylan_11

Dylan_12

Dylan_13

Dylan_14

Dylan_15

Dylan_16

Dylan_17

Dylan_18

Dylan_19

Dylan_20
(Primase/Polymerase)

Dylan_21

Dylan_22

Dylan_23
(WhiB)

Dylan_24

Dylan_25

Dylan_26

Dylan_27

Dylan_28

Dylan_29

Dylan_30
(Terminase Large Subunit)

Dylan_31
(Portal)

Dylan_32
(O-methyltransferase)

Dylan_33

Dylan_34
(Glycosyltransferase)

Dylan_35
(Glycosyltransferase)

Dylan_36
(Prohead Protease)

Dylan_37

Dylan_38
(Capsid)

Dylan_39

Dylan_40

Dylan_41

Dylan_42

Dylan_43

Dylan_44

Dylan_45

Dylan_46
(Major Tail Subunit)

Dylan_47

Dylan_48

Dylan_49

Dylan_50

Dylan_51
(DNA Binding Protein)

Dylan_52

Dylan_53
(Tail Assembly Chaperone)

Dylan_54
(Tail Assembly Chaperone)

Dylan_55
(Tapemeasure)

Dylan_56
(Minor Tail Subunit)

Dylan_57
(Minor Tail Subunit)

Dylan_58
(Minor Tail Subunit)

Dylan_59
(D-ala-D-ala-Carboxypeptidase)

Dylan_60
(Minor Tail Subunit)

Dylan_61
(Minor Tail Subunit)

Dylan_62

Dylan_63

Dylan_64

Dylan_65

Dylan_66
(LysA)

Dylan_67
(LysB)

Dylan_68
(Holin)

Dylan_69

Dylan_70

Dylan_71

Dylan_72

Dylan_73
(DNA Binding Protein)

Dylan_74

Dylan_75

Dylan_76

Dylan_77

Dylan_78

Dylan_79

Dylan_80

Dylan_81

Dylan_82

Dylan_83
(DNA Pol III Beta Subunit)

Dylan_84

Dylan_85

Dylan_86

Dylan_87
(Ku)

Dylan_88

Dylan_89
(ParB-like Protein)

Dylan_90

Dylan_91

Dylan_92

Dylan_93

Dylan_94

Dylan_95
(AAA ATPase)

Dylan_96

Dylan_97

Dylan_98

Dylan_99

Dylan_100

Dylan_101

Dylan_102

Dylan_103

Dylan_104

Dylan_105

Dylan_106

Dylan_107

Dylan_108

Dylan_109

Dylan_110

Dylan_111

Dylan_112

Dylan_113

Dylan_114

Dylan_115

Dylan_116

Dylan_117

Dylan_118

Dylan_119

Dylan_120

Dylan_121

Publication Info
Uploaded to GenBank?Yes
GenBank AccessionKF024730
Refseq NumberNC_022325
Published in PaperPope, Bowman, Russell, et al 2015, eLIFE
Available Files
Plaque PictureDownload
EM PictureDownload
Final DNAMaster FileDownload