Microbacterium phage ASegato
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Detailed Information for Phage ASegato
Discovery Information
Isolation HostMicrobacterium foliorum NRRL B-24224
Found ByBeatriz De Oliveira Segato
Year Found2022
Location FoundQuincy, MA USA
Finding InstitutionEastern Nazarene College
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?No
Isolation Temperature23°C
GPS Coordinates42.27307 N, 71.01037 W Map
Discovery NotesSoil was dry but the grass was very green, alive and overgrown
Naming NotesMy mother's name is Aurea and our family last name is Segato
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedJan 3, 2023
Sequencing FacilityPittsburgh Bacteriophage Institute
Shotgun Sequencing MethodIllumina Sequencing
Approximate Shotgun Coverage754
Genome length (bp)63246
Character of genome endsDirect Terminal Repeat
Direct Terminal Repeat Length3797 bp
GC Content61.7%
Fasta file available?Yes: Download fasta file
Characterization
ClusterED
SubclusterED2
Cluster Life CycleLytic
Other Cluster Members
Annotating InstitutionEastern Nazarene College
Annotation StatusIn GenBank
Plaque NotesPhages were medium size with a clear center and a cloudy ring around it and clear all around the ring.

Bulls eye phage
MorphotypeSiphoviridae
Has been Phamerated?Yes
Gene List

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ASegato_1

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ASegato_24

ASegato_25

ASegato_29

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ASegato_33

ASegato_34
(NrdH-like glutaredoxin)

ASegato_35
(phosphoesterase)

ASegato_36
(HNH endonuclease)

ASegato_37

ASegato_38
(terminase)

ASegato_39
(portal protein)

ASegato_40
(capsid maturation protease)

ASegato_41

ASegato_42
(major capsid protein)

ASegato_43

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ASegato_45

ASegato_46
(minor tail protein)

ASegato_47
(head-to-tail stopper)

ASegato_48

ASegato_49
(tail terminator)

ASegato_50

ASegato_51

ASegato_52
(major tail protein)

ASegato_53
(tail assembly chaperone)

ASegato_54
(tail assembly chaperone)

ASegato_55
(tape measure protein)

ASegato_56
(minor tail protein)

ASegato_57
(minor tail protein)

ASegato_58

ASegato_59

ASegato_60

ASegato_61
(endolysin)

ASegato_62

ASegato_63

ASegato_64
(RuvC-like resolvase)

ASegato_65

ASegato_66

ASegato_67

ASegato_68

ASegato_69
(DNA primase/polymerase)

ASegato_70

ASegato_71

ASegato_72

ASegato_73
(exonuclease)

ASegato_74
(ERF family DNA pairing protein)

ASegato_75

ASegato_76
(DnaQ-like DNA polymerase III subunit)

ASegato_77

ASegato_78

ASegato_79
(RNA ligase)

ASegato_80
(polynucleotide kinase)

ASegato_81

ASegato_82
(DNA helicase)

ASegato_83

ASegato_84

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ASegato_92

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ASegato_94

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ASegato_96

ASegato_97

ASegato_98

ASegato_99
(DNA binding domain protein)

ASegato_100

ASegato_101

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ASegato_124

Submitted Annotation in DNA Master FormatDownload
Submitted Minimal DNA Master FileDownload
Author ListDownload
Annotation Cover SheetDownload
Publication Info
Uploaded to GenBank?Yes
GenBank AccessionOR521059
Refseq NumberNone yet
Archiving Info
Archiving status Archived
SEA Lysate Titer 1.88x10^8
Pitt Freezer Box# 152
Pitt Freezer Box Grid# C2
Available Files
Plaque PictureDownload
Restriction Digest PictureDownload
EM PictureDownload
GenBank File for PhameratorDownload