Microbacterium phage Pioneer3
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Detailed Information for Phage Pioneer3
Discovery Information
Isolation HostMicrobacterium paraoxydans NWU1
Found ByMorgan A. Shipley
Year Found2016
Location FoundLincoln, NE USA
Finding InstitutionNebraska Wesleyan University
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?Unknown
Isolation TemperatureNot entered
GPS Coordinates40.8 N, 96.6 W Map
Discovery NotesThe environmental sample was from a tree berm outside of a dormitory at Nebraska Wesleyan University. This sample produced the phage that completed the program.
Naming NotesI named this phage Pioneer3 because it is the name of the dormitory I live in, as well as where my original environmental sample came from to produce my phage, and 3 is my lucky number.
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedJan 25, 2017
Sequencing FacilityPittsburgh Bacteriophage Institute
Shotgun Sequencing MethodIllumina Sequencing
Approximate Shotgun Coverage1255
Genome length (bp)62954
Character of genome endsDirect Terminal Repeat
Direct Terminal Repeat Length3191 bp
GC Content64.6%
Fasta file available?Yes: Download fasta file
Characterization
ClusterED
SubclusterED1
Cluster Life CycleLytic
Other Cluster Members
Annotating InstitutionNebraska Wesleyan University
Annotation StatusIn GenBank
Plaque NotesThe plaques are about 2 mm in diameter and clear.
MorphotypeSiphoviridae
Has been Phamerated?Yes
Gene List

Click to View

Pioneer3_1

Pioneer3_2

Pioneer3_3

Pioneer3_4

Pioneer3_5

Pioneer3_6

Pioneer3_7

Pioneer3_8

Pioneer3_9

Pioneer3_10

Pioneer3_11

Pioneer3_12

Pioneer3_13

Pioneer3_14

Pioneer3_15

Pioneer3_16

Pioneer3_17

Pioneer3_18

Pioneer3_22

Pioneer3_23

Pioneer3_24

Pioneer3_25

Pioneer3_26

Pioneer3_27

Pioneer3_28
(thioredoxin)

Pioneer3_29
(exonuclease)

Pioneer3_30

Pioneer3_31
(terminase)

Pioneer3_32
(portal protein)

Pioneer3_33
(MuF-like minor capsid protein)

Pioneer3_34

Pioneer3_35
(major capsid protein)

Pioneer3_36

Pioneer3_37

Pioneer3_38
(metallophosphoesterase)

Pioneer3_39

Pioneer3_40
(head-to-tail stopper)

Pioneer3_41

Pioneer3_42
(tail terminator)

Pioneer3_43

Pioneer3_44

Pioneer3_45

Pioneer3_46
(tail assembly chaperone)

Pioneer3_47
(tail assembly chaperone)

Pioneer3_48
(tape measure protein)

Pioneer3_49

Pioneer3_50
(minor tail protein)

Pioneer3_51

Pioneer3_52

Pioneer3_53

Pioneer3_54
(lysin A)

Pioneer3_55

Pioneer3_56

Pioneer3_57
(RuvC-like resolvase)

Pioneer3_58

Pioneer3_59

Pioneer3_60

Pioneer3_61

Pioneer3_62

Pioneer3_63
(DNA primase)

Pioneer3_65

Pioneer3_66

Pioneer3_67

Pioneer3_68
(exonuclease)

Pioneer3_69

Pioneer3_70
(exonuclease)

Pioneer3_71

Pioneer3_72

Pioneer3_73

Pioneer3_74
(RNA ligase)

Pioneer3_75

Pioneer3_76
(polynucleotide kinase)

Pioneer3_77
(DNA helicase)

Pioneer3_78

Pioneer3_79

Pioneer3_80

Pioneer3_81

Pioneer3_82

Pioneer3_83

Pioneer3_84

Pioneer3_85

Pioneer3_86

Pioneer3_87

Pioneer3_88

Pioneer3_89

Pioneer3_90

Pioneer3_91

Pioneer3_92

Pioneer3_93

Pioneer3_94

Pioneer3_95

Pioneer3_96

Pioneer3_97

Pioneer3_98
(helix-turn-helix DNA binding domain protein)

Pioneer3_99

Pioneer3_100

Pioneer3_101

Pioneer3_102

Pioneer3_103

Pioneer3_104

Pioneer3_105

Pioneer3_106

Pioneer3_107

Pioneer3_108

Pioneer3_109

Pioneer3_110

Pioneer3_111

Pioneer3_112

Pioneer3_113

Pioneer3_114

Pioneer3_115

Pioneer3_116

Pioneer3_117

Pioneer3_118

Submitted Minimal DNA Master FileDownload
Publication Info
Uploaded to GenBank?Yes
GenBank AccessionMH271310
Refseq NumberNone yet
Archiving Info
Archiving status Archived
SEA Lysate Titer 3.3x10^10
Date of SEA Lysate Titering Nov 8, 2016
Pitt Freezer Box# 45
Pitt Freezer Box Grid# F9
Available Files
Plaque PictureDownload
Restriction Digest PictureDownload
EM PictureDownload
Final DNAMaster FileDownload
GenBank File for PhameratorDownload