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Microbacterium phage Lupine
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Detailed Information for Phage Lupine
Discovery Information
Isolation HostMicrobacterium foliorum NRRL B-24224 SEA
Former namesN/A
Found ByNatalie Hanson and Leah Watson
Year Found2018
Location FoundOxford, OH USA
Finding InstitutionMiami University
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?Yes
Isolation Temperature30°C
GPS Coordinates39.537222 N, 84.7325 W Map
Discovery NotesThe sample was taken from under pine straw around a conservation area sign for Helen S. Ruder Preserve across from Yager Stadium.
Naming NotesOur original sample did not produce plaques, and our second sample disitegrated in a biodegradable flask during enrichment; therefore, our professor gave us a sample taken from July 2018. The sample was taken from pine straw. For this reason, our name of our phage is Lupine for the "pine" straw. Also, Leah's favorite Harry Potter chapter is Professor Lupus; this inspired our name, too.
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedJan 16, 2019
Sequencing FacilityPittsburgh Bacteriophage Institute
Shotgun Sequencing MethodIllumina Sequencing
Approximate Shotgun Coverage1494
Genome length (bp)62533
Character of genome endsDirect Terminal Repeat
Direct Terminal Repeat Length3260 bp
GC Content62.7%
Fasta file available?Yes: Download fasta file
Characterization
ClusterED
SubclusterED1
Cluster Life CycleLytic
Lysogeny NotesOur phage was most likely lytic, as the bacteria was completely lysed and the plaques were clear in the middle.
Other Cluster Members
Annotation StatusIn GenBank
Plaque NotesThe plaque appeared to have a double ring. The small, outer ring is turbid, while the larger, inner ring is clear. The plaques all had similar morphology with defined borders. The plaque morphology and shape remained constant throughout the entire experiment.
MorphotypeSiphoviridae
Has been Phamerated?Yes
Gene List

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Submitted Minimal DNA Master FileDownload
Publication Info
Uploaded to GenBank?Yes
GenBank AccessionMK919474
Refseq NumberNone yet
Archiving Info
Archiving status Archived
SEA Lysate Titer 1.11x10^12
Pitt Freezer Box# 74
Pitt Freezer Box Grid# C10
Available Files
Plaque PictureDownload
Restriction Digest PictureDownload
EM PictureDownload
GenBank File for PhameratorDownload