Propionibacterium phage Aquarius

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Detailed Information for Phage Aquarius
Discovery Information
Isolation HostPropionibacterium acnes ATCC 6919
Found ByTeam We Phaged Up
Year Found2016
Location FoundLos Angeles, CA USA
Finding InstitutionUniversity of California, Los Angeles
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?Unknown
Isolation Temperature37°C
GPS CoordinatesUnavailable
Discovery NotesPhage isolation, characterization, and annotation were performed by Team We Phaged Up: Stacee Mendonca, Christine Nguyen, Cameron Safarpour, and Stephanie Wottrich.

The phage was isolated by direct plating of nasal microcomedones obtained by a Biore pore strip from the nose of Stacee Mendonca.

TAs: Rocky Ng and Andrew Lund; UA: Lariska Theron
Instructors: Dr. Frances Mercer, Dr. Krisanavane Reddi, and Dr. Jordan Moberg Parker.
Naming NotesStacee's astrological sign is Aquarius. The phage is named after her.
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedAug 26, 2016
Sequencing FacilityPittsburgh Bacteriophage Institute
Shotgun Sequencing MethodIllumina Sequencing
Approximate Shotgun Coverage2841
Genome length (bp)30112
Character of genome ends3' Sticky Overhang
Overhang Length11 bases
Overhang SequenceTCGTACGGCTT
GC Content54.5%
Fasta file available?Available upon entering GPS coordinates
Characterization
ClusterBU
Subcluster--
Cluster Life CycleLytic
Lysogeny NotesThe phage appears lytic at first glance due to almost exclusively producing completely clear plaques on the bacterial law. However, bacterial growth was found to be present in the centers of plaques for certain clinical isolates of P. acnes analyzed from the results of the host range assay and select plates of MINI and MIDI phage lysates. This bacteria was isolated and tested for lysogenic character. Patch testing suggested the phage was indeed lysogenic, and spot testing suggested that the phage was also immune to superinfection in certain clinical isolates upon reinfection with the same phage (Aquarius). PCR was conducted using specific primers which correspond to genomic ends that are likely capable of circularization, which yielded a characteristic ~0.75 kb (~735 bp) band on the gel, suggesting the capacity of Aquarius for undergoing a pseudolysogenic life cycle.
Other Cluster Members
Annotating InstitutionUnknown or unassigned
Annotation StatusIn GenBank
Plaque NotesClear 3.5 mm plaques
MorphotypeSiphoviridae
Has been Phamerated?Yes
Gene List
Submitted Minimal DNA Master FileDownload
Publication Info
Uploaded to GenBank?Yes
GenBank AccessionMF919491
Refseq NumberNone yet
Archiving Info
Archiving status Archived
Pitt Freezer Box# 28
Pitt Freezer Box Grid# E2
Available Files
Plaque PictureDownload
Restriction Digest PictureDownload
EM PictureDownload
GenBank File for PhameratorDownload