| Detailed Information for Phage Aquarius | |
| Discovery Information | |
| Isolation Host | Propionibacterium acnes ATCC 6919 |
| Found By | Team We Phaged Up |
| Year Found | 2016 |
| Location Found | Los Angeles, CA USA |
| Finding Institution | University of California, Los Angeles |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Unknown |
| Isolation Temperature | 37°C |
| GPS Coordinates | 34.0689 N, 118.4452 W Map |
| Discovery Notes | Phage isolation, characterization, and annotation were performed by Team We Phaged Up: Stacee Mendonca, Christine Nguyen, Cameron Safarpour, and Stephanie Wottrich. The phage was isolated by direct plating of nasal microcomedones obtained by a Biore pore strip. TAs: Rocky Ng and Andrew Lund; UA: Lariska Theron Instructors: Dr. Frances Mercer, Dr. Krisanavane Reddi, and Dr. Jordan Moberg Parker. |
| Naming Notes | Stacee's astrological sign is Aquarius. The phage is named after her. |
| Sequencing Information | |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Aug 26, 2016 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina Sequencing |
| Approximate Shotgun Coverage | 2841 |
| Genome length (bp) | 30112 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 11 bases |
| Overhang Sequence | TCGTACGGCTT |
| GC Content | 54.5% |
| Fasta file available? | Yes: Download fasta file |
| Characterization | |
| Cluster | BU |
| Subcluster | -- |
| Cluster Life Cycle | Lytic |
| Lysogeny Notes | The phage appears lytic at first glance due to almost exclusively producing completely clear plaques on the bacterial law. However, bacterial growth was found to be present in the centers of plaques for certain clinical isolates of P. acnes analyzed from the results of the host range assay and select plates of MINI and MIDI phage lysates. This bacteria was isolated and tested for lysogenic character. Patch testing suggested the phage was indeed lysogenic, and spot testing suggested that the phage was also immune to superinfection in certain clinical isolates upon reinfection with the same phage (Aquarius). PCR was conducted using specific primers which correspond to genomic ends that are likely capable of circularization, which yielded a characteristic ~0.75 kb (~735 bp) band on the gel, suggesting the capacity of Aquarius for undergoing a pseudolysogenic life cycle. |
| Other Cluster Members |
Click to ViewAquarius ATCC29399BC ATCC29399BT Attacne BruceLethal Cota DrParker Enochoraptor Enoki Keiki Kubed Lauchelly Leviosa LilBandit MEAK Moyashi MrAK Ouroboros P1.1 P100.1 P100A P100D P101A P104A P104B P105 P106A P106C P106I P106L P106M P107A P107C P108C P14.4 P9.1 PA6 PAD20 PAS50 Pirate Procrass1 QueenBey Rileysaurus SKKY Solid Stormborn Supernova Wizzo |
| Annotating Institution | Unknown or unassigned |
| Annotation Status | In GenBank |
| Plaque Notes | Clear 3.5 mm plaques |
| Morphotype | Siphoviridae |
| Has been Phamerated? | Yes |
| Gene List |
Click to ViewAquarius_1 Aquarius_2 Aquarius_3 Aquarius_4 Aquarius_5 Aquarius_6 Aquarius_7 Aquarius_8 Aquarius_9 Aquarius_10 Aquarius_11 Aquarius_12 Aquarius_13 Aquarius_14 Aquarius_15 Aquarius_16 Aquarius_17 Aquarius_18 Aquarius_19 Aquarius_20 Aquarius_21 Aquarius_22 Aquarius_23 Aquarius_24 Aquarius_25 Aquarius_26 Aquarius_27 Aquarius_28 Aquarius_29 Aquarius_30 Aquarius_31 Aquarius_32 Aquarius_33 Aquarius_34 Aquarius_35 Aquarius_36 Aquarius_37 Aquarius_38 Aquarius_39 Aquarius_40 Aquarius_41 Aquarius_42 Aquarius_43 Aquarius_44 Aquarius_45 Aquarius_46 Aquarius_47 Aquarius_48 |
| Submitted Minimal DNA Master File | Download |
| Publication Info | |
| Uploaded to GenBank? | Yes |
| GenBank Accession | MF919491 |
| Refseq Number | None yet |
| Archiving Info | |
| Archiving status | Archived |
| Pitt Freezer Box# | 28 |
| Pitt Freezer Box Grid# | E2 |
| Available Files | |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| EM Picture | Download |
| GenBank File for Phamerator | Download |