Propionibacterium phage Leviosa
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Detailed Information for Phage Leviosa
Discovery Information
Isolation HostPropionibacterium acnes ATCC 6919
Found ByTeams YCPWU and Plaquille O'Neil
Year Found2015
Location FoundLos Angeles, CA USA
Finding InstitutionUniversity of California, Los Angeles
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?No
Isolation Temperature37°C
GPS Coordinates34.068900 N, 118.445200 W Map
Discovery NotesMicrocomedones (blackheads) were extracted from the nose of Andy Vu using a Biore Pore Strip on 04/06/2015. The skin was unwashed and slightly oily, and no moisturizer had been applied. Sample was enriched in RCM media with P. acnes strain ATCC 6919 under anaerobic conditions at 37oC.

Phage Leviosa was isolated,characterized, and annotated by Team ​Plaquille O’Neal and Team You Can’t Phage with Us (YCPWU).

Plaquille O'Neal: Connie Giang, Adilene Barajas, Andy Vu, Samantha Kaufhold
YCPWU: Aparna Vancheswaran, Lariska Theron, Naeha Lakshmanan, Suruchi Salgar
TA: Rocky Ng
Instructor: Jordan Moberg Parker

Of Note: Leviosa was able to lyse phage resistant P. acnes strain B101.9 (ribotype 2), which is known to contain CRISPR loci, and was not able to be lysed by other class phages, Cota and LilBandit.
Naming NotesNamed after the spell in Harry Potter.
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedSep 11, 2015
Sequencing FacilityNC State Genomic Sciences Laboratory
Shotgun Sequencing MethodIllumina Sequencing
Approximate Shotgun Coverage6276
Genome length (bp)29451
Character of genome ends3' Sticky Overhang
Overhang Length11 bases
Overhang SequenceTCGTACGGCTT
GC Content53.7%
Fasta file available?Yes: Download fasta file
Cluster Life CycleLytic
Lysogeny NotesLeviosa is pseudolysogenic. The genome does not contain any known lysogeny genes, but lysogens that shed phage are able to be cultivated.
Other Cluster Members
Annotating InstitutionUnknown or unassigned
Annotation StatusIn GenBank
Plaque NotesBullseye morphology
Average diameter: 8.5 mm
Size range:7­10 mm
Number of Genes45
Has been Phamerated?Yes
Gene List
Submitted Annotation in DNA Master FormatDownload
Submitted Minimal DNA Master FileDownload
Author ListDownload
Annotation Cover SheetDownload
Publication Info
Uploaded to GenBank?Yes
GenBank AccessionMF919515
Refseq NumberNone yet
Archiving Info
Archiving status Archived
Pitt Freezer Box# 23
Pitt Freezer Box Grid# E5
Available Files
Plaque PictureDownload
EM PictureDownload
GenBank File for PhameratorDownload