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Detailed Information for Phage MEAK
Discovery Information
Isolation HostPropionibacterium acnes ATCC 6919
Found ByBaby Got Plaque
Year Found2016
Location FoundLos Angeles, CA USA
Finding InstitutionUniversity of California, Los Angeles
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?Unknown
Isolation Temperature37°C
GPS Coordinates34.068582 N, 118.441285 W Map
Discovery NotesPhage isolation, characterization, and annotation were performed by Team Baby Got Plaque: Aida Akopyan, Emily Pang, Kei Shao, and Meera Solanki.

The phage was isolated by direct plating of nasal microcomedones obtained by a Biore pore strip from the nose of Meera Solanki.

TAs: Rocky Ng, Andrew Lund
UA: Lariska Theron
Instructors: Dr. Frances Mercer, Dr. Krisanavane Reddi and Dr. Jordan Moberg Parker.
Naming NotesThe students combined their first letter of their names to create MEAK (Meera, Emily, Aida, and Kei).
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedAug 26, 2016
Sequencing FacilityPittsburgh Bacteriophage Institute
Shotgun Sequencing MethodIllumina Sequencing
Approximate Shotgun Coverage5746
Genome length (bp)29223
Character of genome ends3' Sticky Overhang
Overhang Length11 bases
Overhang SequenceTCGTACGGCTT
GC Content54.0%
Fasta file available?Yes: Download fasta file
Characterization
ClusterBU
Subcluster--
Cluster Life CycleLytic
Lysogeny NotesPlaque morphology and lack of lysogeny genes suggest this is a pseudolysogenic phage.
Other Cluster Members
Annotating InstitutionUnknown or unassigned
Annotation StatusIn GenBank
Plaque NotesClear plaques with turbid centers.
Size range between 2-5mm, average 3 mm in diameter.
MorphotypeSiphoviridae
Has been Phamerated?Yes
Gene List
Submitted Minimal DNA Master FileDownload
Publication Info
Uploaded to GenBank?Yes
GenBank AccessionMF919522
Refseq NumberNone yet
Archiving Info
Archiving status Archived
Pitt Freezer Box# 28
Pitt Freezer Box Grid# D12
Available Files
Plaque PictureDownload
EM PictureDownload
GenBank File for PhameratorDownload