| Detailed Information for Phage BarretLemon |
| Discovery Information |
| Isolation Host | Arthrobacter sp. ATCC 21022 |
| Found By | Emily Lehmann and Elizabeth Kriese |
| Year Found | 2013 |
| Location Found | Chippewa Falls, WI USA |
| Finding Institution | University of Wisconsin-River Falls |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | Not entered |
| GPS Coordinates | 44.938522 N, 91.388147 W Map |
| Discovery Notes | The soil was collected on September 22, 2013 at 3:30 pm in Chippewa Falls WI. It is garden soil composed of horse feces and table scraps located under grass clippings near thriving tomato plants. The soil was very loose and moist. It was a cool 66 degree Fahrenheit sunny fall day. |
| Naming Notes | The first part of the name is named after Elizabeth's seventh grade biology teacher who inspired her to persue a career in Biology. The seond part of the name is named after Emily's last name. In high school all of her friends would call her Emily Lemon. |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Jan 16, 2015 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina Sequencing |
| Approximate Shotgun Coverage | 4324 |
| Genome length (bp) | 51290 |
| Character of genome ends | Unknown |
| GC Content | 60.9% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | AO |
| Subcluster | AO2 |
| Cluster Life Cycle | Lytic |
| Other Cluster Members |
|
| Annotating Institution | Unknown or unassigned |
| Annotation Status | In GenBank |
| Plaque Notes | Emily and Elizabeth took one soil sample and obtained plaques of varying sizes. Elizabeth decided to go forward testing the larger plaques while Emily continued to test the smaller ones. After various comparing various test results we have concluded that it is indeed the same plaque that has varying plaque sizes. |
| Morphotype | Myoviridae |
| Number of Genes | 79 |
| Number of tRNAs | 0 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | KU647629 |
| Refseq Number | None yet |
| Archiving Info |
| Archiving status |
Archived |
| SEA Lysate Titer |
4.3X10^9 |
| Date of SEA Lysate Titering |
Oct 22, 2013 |
| Pitt Freezer Box# |
147 |
| Pitt Freezer Box Grid# |
F12 |
| Available Files |
| Final DNAMaster File | Download |