| Detailed Information for Phage BigDome | |
| Discovery Information | |
| Isolation Host | Arthrobacter sp. ATCC 21022 |
| Found By | Jacob Khuu |
| Year Found | 2024 |
| Location Found | Los Angeles, CA USA |
| Finding Institution | University of California, Los Angeles |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | 30°C |
| GPS Coordinates | 34.0722 N, 118.4294 W Map |
| Discovery Notes | Host-Enrichment using Arthrobacter sulfureus, Arthrobacter sp., A. atrocyaneus, and Arthrobacter globiformis. The soil sample containing the phage was incubated at 30 degrees Celsius for 15 hours with the host bacteria mixture. The mixture was then plated and incubated for 30 degrees Celsius for 48 hours to elicit plaque formation for observation. Sample description: Moist soil Depth from which sample was taken: 3 cm Air temperature if sample collected outdoors: 22 C AL Quarter and Lab section: F24-1B Instructor: Dr. Tejas Bouklas & Dr. Daniel Velez-Ramirez Teaching Assistant: Praneel Bonthala Undergraduate Assistant(s): Naye Ramos & Aaron Holaway Team #1 Name: We Got Lyse (WGL) Team #1 Members: Sarahi Jasso, Xeleste Aguilar, Ananya Sridharan, & Mallika Mathew |
| Naming Notes | We named it BigDome since “Dome” means head, and phages have a large head attached to a tail. We presumed our phage would have a big head. |
| Sequencing Information | |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Dec 16, 2024 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina |
| Approximate Shotgun Coverage | 2695 |
| Genome length (bp) | 51084 |
| Character of genome ends | Circularly Permuted |
| GC Content | 60.9% |
| Fasta file available? | Yes: Download fasta file |
| Characterization | |
| Cluster | AO |
| Subcluster | AO2 |
| Cluster Life Cycle | Lytic |
| Other Cluster Members |
Click to ViewEastWest (None) Beans (AO1) Brent (AO1) Franzy (AO1) Jawnski (AO1) King2 (AO1) NathanVaag (AO1) Piccoletto (AO1) BarretLemon (AO2) BigDome (AO2) BossLady (AO2) GravityBall (AO2) Grekaycon (AO2) JKerns (AO2) Jordan (AO2) LeeroyJ (AO2) Martha (AO2) Pippa (AO2) Shade (AO2) Sicarius2 (AO2) Sonny (AO2) StevieBAY (AO2) TaeYoung (AO2) Timinator (AO2) Wyborn (AO2) Zartrosa (AO2) Abba (AO3) Nostromo (AO3) |
| Annotating Institution | University of California, Los Angeles |
| Annotation Status | Being Annotated (Expected completion by 5/1/2025) |
| Plaque Notes | Description of plaque morphology: clear, circular, defined edges Average size (and size range) diameter in mm: 3mm |
| Morphotype | Siphoviridae |
| Number of Genes | 79 |
| Number of tRNAs | 0 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
Click to ViewBigDome_1 BigDome_2 BigDome_3 BigDome_4 BigDome_5 BigDome_6 BigDome_7 BigDome_8 BigDome_9 BigDome_10 BigDome_11 BigDome_12 BigDome_13 BigDome_14 BigDome_15 BigDome_16 BigDome_17 BigDome_18 BigDome_19 BigDome_20 BigDome_21 BigDome_22 BigDome_23 BigDome_24 BigDome_25 BigDome_26 BigDome_27 BigDome_28 BigDome_29 BigDome_30 BigDome_31 BigDome_32 BigDome_33 BigDome_34 BigDome_35 BigDome_36 BigDome_37 BigDome_38 BigDome_39 BigDome_40 BigDome_41 BigDome_42 BigDome_43 BigDome_44 BigDome_45 BigDome_46 BigDome_47 BigDome_48 BigDome_49 BigDome_50 BigDome_51 BigDome_52 BigDome_53 BigDome_54 BigDome_55 BigDome_56 BigDome_57 BigDome_58 BigDome_59 BigDome_60 BigDome_61 BigDome_62 BigDome_63 BigDome_64 BigDome_65 BigDome_66 BigDome_67 BigDome_68 BigDome_69 BigDome_70 BigDome_71 BigDome_72 BigDome_73 BigDome_74 BigDome_75 BigDome_76 BigDome_77 BigDome_78 BigDome_79 |
| Publication Info | |
| Uploaded to GenBank? | No |
| GenBank Accession | None yet |
| Refseq Number | None yet |
| Archiving Info | |
| Archiving status | Not in Pitt Archives |
| Available Files | |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| EM Picture | Download |
