Arthrobacter phage BigDome
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| Detailed Information for Phage BigDome | |
| Discovery Information | |
| Isolation Host | Arthrobacter sp. ATCC 21022 |
| Found By | Jacob Khuu |
| Year Found | 2024 |
| Location Found | Los Angeles, CA USA |
| Finding Institution | University of California, Los Angeles |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | 30°C |
| GPS Coordinates | 34.0722 N, 118.4294 W Map |
| Discovery Notes | Host-Enrichment using Arthrobacter sulfureus, Arthrobacter sp., A. atrocyaneus, and Arthrobacter globiformis. The soil sample containing the phage was incubated at 30 degrees Celsius for 15 hours with the host bacteria mixture. The mixture was then plated and incubated for 30 degrees Celsius for 48 hours to elicit plaque formation for observation. Sample description: Moist soil Depth from which sample was taken: 3 cm Air temperature if sample collected outdoors: 22 C AL Quarter and Lab section: F24-1B Instructor: Dr. Tejas Bouklas & Dr. Daniel Velez-Ramirez Teaching Assistant: Praneel Bonthala Undergraduate Assistant(s): Naye Ramos & Aaron Holaway Team #1 Name: We Got Lyse (WGL) Team #1 Members: Sarahi Jasso, Xeleste Aguilar, Ananya Sridharan, & Mallika Mathew |
| Naming Notes | We named it BigDome since “Dome” means head, and phages have a large head attached to a tail. We presumed our phage would have a big head. |
| Sequencing Information | |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Dec 16, 2024 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina |
| Approximate Shotgun Coverage | 2695 |
| Genome length (bp) | 51084 |
| Character of genome ends | Circularly Permuted |
| GC Content | 60.9% |
| Fasta file available? | Yes: Download fasta file |
| Characterization | |
| Cluster | AO |
| Subcluster | AO2 |
| Cluster Life Cycle | Lytic |
| Other Cluster Members |
Click to ViewEastWest (None) Beans (AO1) Brent (AO1) Franzy (AO1) Jawnski (AO1) King2 (AO1) NathanVaag (AO1) Piccoletto (AO1) BarretLemon (AO2) BigDome (AO2) BossLady (AO2) GravityBall (AO2) Grekaycon (AO2) JKerns (AO2) Jordan (AO2) LeeroyJ (AO2) Martha (AO2) Pippa (AO2) Shade (AO2) Sicarius2 (AO2) Sonny (AO2) StevieBAY (AO2) TaeYoung (AO2) Timinator (AO2) Wyborn (AO2) Zartrosa (AO2) Abba (AO3) |
| Annotating Institution | University of California, Los Angeles |
| Annotation Status | Being Annotated (Expected completion by 5/1/2025) |
| Plaque Notes | Description of plaque morphology: clear, circular, defined edges Average size (and size range) diameter in mm: 3mm |
| Morphotype | Siphoviridae |
| Has been Phamerated? | No |
| Publication Info | |
| Uploaded to GenBank? | No |
| GenBank Accession | None yet |
| Refseq Number | None yet |
| Archiving Info | |
| Archiving status | Not in Pitt Archives |
| Available Files | |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| EM Picture | Download |
