| Detailed Information for Phage BlueOtter |
| Discovery Information |
| Isolation Host | Streptomyces lividans JI 1326 |
| Found By | Ryan Williamson,Nathaniel John |
| Year Found | 2023 |
| Location Found | St. Louis, MO United States |
| Finding Institution | Washington University in St. Louis |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | No |
| Isolation Temperature | 27°C |
| GPS Coordinates | 38.644137 N, 90.315218 W Map |
| Discovery Notes | Soil later observed to contain Phage BlueOtter was obtained from the South 40 on the Washington University in St. Louis campus. It was gathered approximately one centimeter below the ground at GPS coordinates 38.644137 N, 90.315218 W. The ambient temperature was about 26.6°C and the environment contained many organics. |
| Naming Notes | Named after our favorite animal and color. |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Feb 20, 2024 |
| Sequencing Facility | The McDonnell Genome Institute at Washington University |
| Shotgun Sequencing Method | Illumina Sequencing |
| Approximate Shotgun Coverage | 162 |
| Genome length (bp) | 132161 |
| Character of genome ends | Direct Terminal Repeat |
| Direct Terminal Repeat Length | 11074 bp |
| GC Content | 50.1% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | BE |
| Subcluster | BE1 |
| Cluster Life Cycle | Lytic |
| Other Cluster Members |
|
| Annotating Institution | Washington University in St. Louis |
| Annotation Status | In GenBank |
| Plaque Notes | Plaques are medium sized, around 2.5 to 3mm in diameter. Edges are rough and not well defined. |
| Number of Genes | 227 |
| Number of tRNAs | 43 |
| Number of tmRNAs | 1 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Minimal DNA Master File | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | PQ114737 |
| Refseq Number | None yet |
| Archiving Info |
| Archiving status |
Not in Pitt Archives |
| SEA Lysate Titer |
1 x 10^11 |
| Available Files |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| EM Picture | Download |
| GenBank File for Phamerator | Download |
