The Actinobacteriophage Database

Streptomyces phage Enygma

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(S. griseus NBRC13350)

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(S. scabiei 82-22)

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Detailed Information for Phage Enygma
Discovery Information
Isolation Host	Streptomyces mirabilis NRRL B-2400
Former names	ThisIsPatrick
Found By	Timothy Agnes
Year Found	2020
Location Found	Catonsville, MD USA
Finding Institution	University of Maryland, Baltimore County
Program	Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?	No
Isolation Temperature	30°C
GPS Coordinates	39.243826 N, 76.722214 W Map
Discovery Notes	Collected in Patapsco State Park in the Avalon area from very moist soil, root filled, surround by organic material, ~3" deep. Collected on 9-02-20.
Naming Notes	Voted on by the class
Sequencing Information
Sequencing Complete?	Yes
Date Sequencing Completed	Jan 14, 2021
Sequencing Facility	Pittsburgh Bacteriophage Institute
Shotgun Sequencing Method	Illumina Sequencing
Approximate Shotgun Coverage	830
Genome length (bp)	133969
Character of genome ends	Direct Terminal Repeat
Direct Terminal Repeat Length	11545 bp
GC Content	48.5%
Sequencing Notes	In about 60% of the sequencing reads for this phage, a 4413 bp deletion (positions 89642 to 94054, inclusive) was observed. Though the population with the deletion is technically the "majority", we've reported the longer sequence as the final genome sequence, otherwise that deleted bit would be lost.
Fasta file available?	Yes: Download fasta file
Characterization
Cluster	BE
Subcluster	BE2
Cluster Life Cycle	Lytic
Other Cluster Members	Click to View Anedea (BE1) Angela (BE1) Bartholomune (BE1) BlueOtter (BE1) Bmoc (BE1) Braelyn (BE1) Cross (BE1) Cursive (BE1) Daubenski (BE1) EGole (BE1) Evy (BE1) HangryHippo (BE1) Jay2Jay (BE1) Larnav (BE1) Leo04 (BE1) Liandry (BE1) LilMartin (BE1) Lululemon (BE1) Mildred21 (BE1) MulchMansion (BE1) Navo (BE1) NootNoot (BE1) PacManQ (BE1) Paradiddles (BE1) Peebs (BE1) Pepperwood (BE1) Persimmon (BE1) PinkiePie (BE1) Samisti12 (BE1) Squillium (BE1) Sushi23 (BE1) Targaryen (BE1) Teutsch (BE1) Tribute (BE1) Warpy (BE1) Watermoore (BE1) WhereRU (BE1) Amabiko (BE2) Battuta (BE2) Birchlyn (BE2) BoomerJR (BE2) Bordeaux (BE2) CeilingFan (BE2) Elmer (BE2) Enygma (BE2) Genie2 (BE2) Gibbi (BE2) IchabodCrane (BE2) JimJam (BE2) Jollison (BE2) Karimac (BE2) KentuckyRacer (BE2) LukeCage (BE2) MindFlayer (BE2) Mugiwara (BE2) PumpkinSpice (BE2) Quaran19 (BE2) SaltySpitoon (BE2) Sollertia (BE2) Spelly (BE2) Spilled (BE2) Stanimal (BE2) Starbow (BE2) StarPlatinum (BE2) Tomas (BE2) TomSawyer (BE2) Wipeout (BE2) Wofford (BE2) Yaboi (BE2)
Annotating Institution	Monmouth College
Annotation Status	In GenBank
Morphotype	Siphoviridae
Has been Phamerated?	Yes
Gene List	Click to View Enygma_1 Enygma_2 Enygma_3 Enygma_4 Enygma_5 Enygma_6 (Lsr2-like DNA bridging protein) Enygma_7 Enygma_8 Enygma_9 Enygma_10 Enygma_11 (ParB-like nuclease domain) Enygma_12 Enygma_13 Enygma_14 Enygma_15 Enygma_16 Enygma_17 Enygma_18 Enygma_19 Enygma_20 Enygma_21 Enygma_22 Enygma_23 Enygma_24 (HNH endonuclease) Enygma_25 Enygma_26 Enygma_27 (ThyX-like thymidylate synthase) Enygma_28 Enygma_29 Enygma_30 Enygma_31 Enygma_32 (DNA binding protein) Enygma_33 Enygma_34 Enygma_35 Enygma_37 (HNH endonuclease) Enygma_38 (hydrolase) Enygma_39 Enygma_40 Enygma_41 (glycosyltransferase) Enygma_42 (ribonucleotide reductase) Enygma_43 (hydrolase) Enygma_44 Enygma_45 Enygma_46 (portal protein) Enygma_47 Enygma_48 (capsid maturation protease) Enygma_49 (major capsid protein) Enygma_50 Enygma_51 (tail fiber) Enygma_52 Enygma_53 Enygma_54 (head-to-tail adaptor) Enygma_55 (head-to-tail stopper) Enygma_56 Enygma_57 Enygma_58 (major tail protein) Enygma_59 (tail assembly chaperone) Enygma_60 Enygma_61 (tape measure protein) Enygma_62 (minor tail protein) Enygma_63 (minor tail protein) Enygma_64 Enygma_65 (tail fiber) Enygma_66 (tailspike protein) Enygma_67 (capsid maturation protease) Enygma_68 Enygma_69 Enygma_70 (HNH endonuclease) Enygma_71 (phosphoesterase) Enygma_72 (holin) Enygma_73 (DNA binding protein) Enygma_74 (helix-turn-helix DNA binding domain) Enygma_75 Enygma_76 Enygma_77 Enygma_78 (DnaB-like dsDNA helicase) Enygma_79 Enygma_80 (DNA primase) Enygma_81 (SSB protein) Enygma_82 (DnaE-like DNA polymerase III (alpha)) Enygma_83 Enygma_84 (MazG-like nucleotide pyrophosphohydrolase) Enygma_85 (RecA-like DNA recombinase) Enygma_86 Enygma_87 (Holliday junction resolvase) Enygma_88 Enygma_89 Enygma_90 Enygma_91 (Cas4 exonuclease) Enygma_92 Enygma_93 (RuvC-like resolvase) Enygma_94 Enygma_95 Enygma_96 (methyltransferase) Enygma_97 (thymidylate kinase) Enygma_98 (oxidoreductase) Enygma_99 (terminase small subunit) Enygma_100 (terminase large subunit) Enygma_101 (HNH endonuclease) Enygma_102 Enygma_106 Enygma_107 Enygma_110 (nucleotidyl transferase) Enygma_111 (FtsK-like DNA translocase) Enygma_112 Enygma_114 Enygma_115 Enygma_117 Enygma_119 Enygma_120 Enygma_121 Enygma_122 Enygma_123 Enygma_124 Enygma_126 Enygma_127 Enygma_128 Enygma_130 Enygma_131 (Ro-like RNA binding protein) Enygma_132 Enygma_133 Enygma_134 Enygma_135 Enygma_136 Enygma_137 Enygma_138 Enygma_139 Enygma_140 Enygma_141 Enygma_143 Enygma_144 Enygma_152 Enygma_153 Enygma_154 Enygma_157 (peptidase) Enygma_158 Enygma_159 Enygma_161 Enygma_162 Enygma_164 Enygma_165 Enygma_166 Enygma_167 (endonuclease VII) Enygma_168 Enygma_172 Enygma_174 Enygma_176 Enygma_179 Enygma_180 Enygma_181 Enygma_185 Enygma_186 Enygma_187 Enygma_192 Enygma_193 (DnaQ-like DNA polymerase III subunit) Enygma_194 Enygma_195 Enygma_196 Enygma_198 Enygma_199 Enygma_200 (acetyltransferase) Enygma_201 Enygma_202 Enygma_204 Enygma_205 Enygma_206 Enygma_207 Enygma_208 Enygma_209 Enygma_210 (RNA ligase) Enygma_211 Enygma_212 Enygma_213 Enygma_214 (polynucleotide kinase) Enygma_215 Enygma_216 Enygma_217 Enygma_218 Enygma_219 Enygma_220 Enygma_222 Enygma_223 Enygma_224 Enygma_226 (SprT-like protease) Enygma_227 Enygma_229 Enygma_231 Enygma_232 Enygma_233 Enygma_234 Enygma_235 Enygma_236 Enygma_237 Enygma_238 (nucleoside deaminase) Enygma_239 (phosphoesterase) Enygma_240 Enygma_241 Enygma_242 (WhiB family transcription factor) Enygma_243 Enygma_244 Enygma_245 Enygma_246 Enygma_247 Enygma_248 Enygma_249 Enygma_250 Enygma_251 Enygma_253 Enygma_254 Enygma_255 Enygma_256 Enygma_257 Enygma_258 (HNH endonuclease) Enygma_259 (thioredoxin) Enygma_260 Enygma_261 Enygma_262 Enygma_263 Enygma_264 Enygma_265 Enygma_266 Enygma_267 Enygma_268 (HNH endonuclease) Enygma_269 Enygma_270 Enygma_271 Enygma_272 Enygma_273 Enygma_274 (Lsr2-like DNA bridging protein) Enygma_275 Enygma_276 Enygma_277 Enygma_278 Enygma_279 (ParB-like nuclease domain) Enygma_280 Enygma_281 Enygma_282 Enygma_283 Enygma_284 Enygma_285 Enygma_286 Enygma_287 Enygma_288 Enygma_289 Enygma_290 Enygma_291 Enygma_292 (HNH endonuclease)
Submitted Annotation in DNA Master Format	Download
Submitted Minimal DNA Master File	Download
Author List	Download
Annotation Cover Sheet	Download
Publication Info
Uploaded to GenBank?	Yes
GenBank Accession	PP358745
Refseq Number	None yet
Archiving Info
Archiving status	Archived
SEA Lysate Titer	1.44e12 pfu/mL
Pitt Freezer Box#	117
Pitt Freezer Box Grid#	A3
Available Files
Plaque Picture	Download
Restriction Digest Picture	Download
EM Picture	Download
GenBank File for Phamerator	Download

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