Streptomyces phage Enygma
Enygma Plaque Picture
Enygma EM Picture
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(S. griseus NBRC13350)
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(S. scabiei 82-22)
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Detailed Information for Phage Enygma
Discovery Information
Isolation HostStreptomyces mirabilis NRRL B-2400
Former namesThisIsPatrick
Found ByTimothy Agnes
Year Found2020
Location FoundCatonsville, MD USA
Finding InstitutionUniversity of Maryland, Baltimore County
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?No
Isolation Temperature30°C
GPS Coordinates39.243826 N, 76.722214 W Map
Discovery NotesCollected in Patapsco State Park in the Avalon area from very moist soil, root filled, surround by organic material, ~3" deep. Collected on 9-02-20.
Naming NotesVoted on by the class
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedJan 14, 2021
Sequencing FacilityPittsburgh Bacteriophage Institute
Shotgun Sequencing MethodIllumina
Sequencer UsedIllumina MiSeq
Read TypeSingle-end reads
Read Length150 bp
Approximate Shotgun Coverage830
Genome length (bp)133969
Character of genome endsDirect Terminal Repeat
Direct Terminal Repeat Length11545 bp
GC Content48.5%
Sequencing NotesIn about 60% of the sequencing reads for this phage, a 4413 bp deletion (positions 89642 to 94054, inclusive) was observed. Though the population with the deletion is technically the "majority", we've reported the longer sequence as the final genome sequence, otherwise that deleted bit would be lost.
Fasta file available?Yes: Download fasta file
Characterization
ClusterBE
SubclusterBE2
Cluster Life CycleLytic
Other Cluster Members

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Anedea (BE1)

Angela (BE1)

Bartholomune (BE1)

BlueOtter (BE1)

Bmoc (BE1)

Braelyn (BE1)

Cadmus (BE1)

Cross (BE1)

Cursive (BE1)

Daubenski (BE1)

EGole (BE1)

Evy (BE1)

HangryHippo (BE1)

Jay2Jay (BE1)

Larnav (BE1)

Leo04 (BE1)

Liandry (BE1)

LilMartin (BE1)

Lululemon (BE1)

Marsus (BE1)

Mildred21 (BE1)

MulchMansion (BE1)

Navo (BE1)

NootNoot (BE1)

PacManQ (BE1)

Paradiddles (BE1)

Peebs (BE1)

Pepperwood (BE1)

Persimmon (BE1)

PinkiePie (BE1)

Riptide (BE1)

Samisti12 (BE1)

Scheme (BE1)

Shuckle (BE1)

Squillium (BE1)

Sushi23 (BE1)

Targaryen (BE1)

Teutsch (BE1)

Tribute (BE1)

Warpy (BE1)

Watermoore (BE1)

WhereRU (BE1)

Amabiko (BE2)

Battuta (BE2)

Birchlyn (BE2)

BoomerJR (BE2)

Bordeaux (BE2)

CeilingFan (BE2)

Elmer (BE2)

Enygma (BE2)

Genie2 (BE2)

Gibbi (BE2)

IchabodCrane (BE2)

JimJam (BE2)

Jollison (BE2)

Karimac (BE2)

KentuckyRacer (BE2)

LukeCage (BE2)

MindFlayer (BE2)

Mugiwara (BE2)

PumpkinSpice (BE2)

Quaran19 (BE2)

Rikishi (BE2)

SaltySpitoon (BE2)

Sollertia (BE2)

Spelly (BE2)

Spilled (BE2)

Stanimal (BE2)

Starbow (BE2)

StarPlatinum (BE2)

Tomas (BE2)

TomSawyer (BE2)

Wipeout (BE2)

Wofford (BE2)

Yaboi (BE2)
Annotating InstitutionMonmouth College
Annotation StatusIn GenBank
MorphotypeSiphoviridae
Number of Genes248
Number of tRNAs43
Number of tmRNAs1
Has been Phamerated?Yes
Gene List

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Enygma_1

Enygma_2

Enygma_3

Enygma_4

Enygma_5

Enygma_6
(Lsr2-like DNA bridging protein)

Enygma_7

Enygma_8

Enygma_9

Enygma_10

Enygma_11
(ParB-like nuclease domain)

Enygma_12

Enygma_13

Enygma_14

Enygma_15

Enygma_16

Enygma_17

Enygma_18

Enygma_19

Enygma_20

Enygma_21

Enygma_22

Enygma_23

Enygma_24
(HNH endonuclease)

Enygma_25

Enygma_26

Enygma_27
(ThyX-like thymidylate synthase)

Enygma_28

Enygma_29

Enygma_30

Enygma_31

Enygma_32
(DNA binding protein)

Enygma_33

Enygma_34

Enygma_35

Enygma_36 — tRNA
(tRNA-Thr(cgt))

Enygma_37
(HNH endonuclease)

Enygma_38
(hydrolase)

Enygma_39

Enygma_40

Enygma_41
(glycosyltransferase)

Enygma_42
(ribonucleotide reductase)

Enygma_43
(hydrolase)

Enygma_44

Enygma_45

Enygma_46
(portal protein)

Enygma_47

Enygma_48
(capsid maturation protease)

Enygma_49
(major capsid protein)

Enygma_50

Enygma_51
(tail fiber)

Enygma_52

Enygma_53

Enygma_54
(head-to-tail adaptor)

Enygma_55
(head-to-tail stopper)

Enygma_56

Enygma_57

Enygma_58
(major tail protein)

Enygma_59
(tail assembly chaperone)

Enygma_60

Enygma_61
(tape measure protein)

Enygma_62
(minor tail protein)

Enygma_63
(minor tail protein)

Enygma_64

Enygma_65
(tail fiber)

Enygma_66
(tailspike protein)

Enygma_67
(capsid maturation protease)

Enygma_68

Enygma_69

Enygma_70
(HNH endonuclease)

Enygma_71
(phosphoesterase)

Enygma_72
(holin)

Enygma_73
(DNA binding protein)

Enygma_74
(helix-turn-helix DNA binding domain)

Enygma_75

Enygma_76

Enygma_77

Enygma_78
(DnaB-like dsDNA helicase)

Enygma_79

Enygma_80
(DNA primase)

Enygma_81
(SSB protein)

Enygma_82
(DnaE-like DNA polymerase III (alpha))

Enygma_83

Enygma_84
(MazG-like nucleotide pyrophosphohydrolase)

Enygma_85
(RecA-like DNA recombinase)

Enygma_86

Enygma_87
(Holliday junction resolvase)

Enygma_88

Enygma_89

Enygma_90

Enygma_91
(Cas4 exonuclease)

Enygma_92

Enygma_93
(RuvC-like resolvase)

Enygma_94

Enygma_95

Enygma_96
(methyltransferase)

Enygma_97
(thymidylate kinase)

Enygma_98
(oxidoreductase)

Enygma_99
(terminase small subunit)

Enygma_100
(terminase large subunit)

Enygma_101
(HNH endonuclease)

Enygma_102

Enygma_103 — tRNA
(tRNA-Gly(tcc))

Enygma_104 — tRNA
(tRNA-Gln(ctg))

Enygma_105 — tRNA
(tRNA-Trp(cca))

Enygma_106

Enygma_107

Enygma_108 — tRNA
(tRNA-Pro(tgg))

Enygma_109 — tRNA
(tRNA-Pro(tgg))

Enygma_110
(nucleotidyl transferase)

Enygma_111
(FtsK-like DNA translocase)

Enygma_112

Enygma_113 — tRNA
(tRNA-Ile(tat))

Enygma_114

Enygma_115

Enygma_116 — tRNA
(tRNA-Ser(gga))

Enygma_117

Enygma_118 — tRNA
(tRNA-Ser(tga))

Enygma_119

Enygma_120

Enygma_121

Enygma_122

Enygma_123

Enygma_124

Enygma_125 — tRNA
(tRNA-Asp(gtc))

Enygma_126

Enygma_127

Enygma_128

Enygma_129 — tRNA
(tRNA-Asp(gtc))

Enygma_130

Enygma_131
(Ro-like RNA binding protein)

Enygma_132

Enygma_133

Enygma_134

Enygma_135

Enygma_136

Enygma_137

Enygma_138

Enygma_139

Enygma_140

Enygma_141

Enygma_142 — tRNA
(tRNA-Glu(ctc))

Enygma_143

Enygma_144

Enygma_145 — tRNA
(tRNA-Glu(ttc))

Enygma_146 — tRNA
(tRNA-Val(tac))

Enygma_147 — tRNA
(tRNA-Leu(tag))

Enygma_148 — tRNA
(tRNA-Leu(tag))

Enygma_149 — tRNA
(tRNA-Phe(gaa))

Enygma_150 — tRNA
(tRNA-?(Thr|Tyr)(gt))

Enygma_151 — tRNA
(tRNA-Gly(gcc))

Enygma_152

Enygma_153

Enygma_154

Enygma_155 — tRNA
(tRNA-Ala(tgc))

Enygma_156 — tRNA
(tRNA-Lys(ctt))

Enygma_157
(peptidase)

Enygma_158

Enygma_159

Enygma_160 — tRNA
(tRNA-Asn(gtt))

Enygma_161

Enygma_162

Enygma_163 — tRNA
(tRNA-Asn(gtt))

Enygma_164

Enygma_165

Enygma_166

Enygma_167
(endonuclease VII)

Enygma_168

Enygma_169 — tRNA
(tRNA-Ala(ggc))

Enygma_170 — tRNA
(tRNA-Lys(ctt))

Enygma_171 — tRNA
(tRNA-Lys(ctt))

Enygma_172

Enygma_173 — tRNA
(tRNA-Lys(ttt))

Enygma_174

Enygma_175 — tRNA
(tRNA-Arg(tcg))

Enygma_176

Enygma_177 — tRNA
(tRNA-Met(cat))

Enygma_178 — tRNA
(tRNA-Met(cat))

Enygma_179

Enygma_180

Enygma_181

Enygma_182 — tRNA
(tRNA-Tyr(gta))

Enygma_183 — tRNA
(tRNA-Tyr(gta))

Enygma_184 — tRNA
(tRNA-His(gtg))

Enygma_185

Enygma_186

Enygma_187

Enygma_188 — tRNA
(tRNA-Cys(gca))

Enygma_189 — tRNA
(tRNA-Thr(tgt))

Enygma_190 — tRNA
(tRNA-Thr(ggt))

Enygma_191 — tRNA
(tRNA-Arg(acg))

Enygma_192

Enygma_193
(DnaQ-like DNA polymerase III subunit)

Enygma_194

Enygma_195

Enygma_196

Enygma_197 — tmRNA
(Tag peptide: ANSNVASAYALAA*)

Enygma_198

Enygma_199

Enygma_200
(acetyltransferase)

Enygma_201

Enygma_202

Enygma_203 — tRNA
(tRNA-?(Ala|Gly)(gc))

Enygma_204

Enygma_205

Enygma_206

Enygma_207

Enygma_208

Enygma_209

Enygma_210
(RNA ligase)

Enygma_211

Enygma_212

Enygma_213

Enygma_214
(polynucleotide kinase)

Enygma_215

Enygma_216

Enygma_217

Enygma_218

Enygma_219

Enygma_220

Enygma_221 — tRNA
(tRNA-Leu(gag))

Enygma_222

Enygma_223

Enygma_224

Enygma_225 — tRNA
(tRNA-Val(gac))

Enygma_226
(SprT-like protease)

Enygma_227

Enygma_228 — tRNA
(tRNA-Leu(caa))

Enygma_229

Enygma_230 — tRNA
(tRNA-Ser(gct))

Enygma_231

Enygma_232

Enygma_233

Enygma_234

Enygma_235

Enygma_236

Enygma_237

Enygma_238
(nucleoside deaminase)

Enygma_239
(phosphoesterase)

Enygma_240

Enygma_241

Enygma_242
(WhiB family transcription factor)

Enygma_243

Enygma_244

Enygma_245

Enygma_246

Enygma_247

Enygma_248

Enygma_249

Enygma_250

Enygma_251

Enygma_252 — tRNA
(tRNA-Gln(ttg))

Enygma_253

Enygma_254

Enygma_255

Enygma_256

Enygma_257

Enygma_258
(HNH endonuclease)

Enygma_259
(thioredoxin)

Enygma_260

Enygma_261

Enygma_262

Enygma_263

Enygma_264

Enygma_265

Enygma_266

Enygma_267

Enygma_268
(HNH endonuclease)

Enygma_269

Enygma_270

Enygma_271

Enygma_272

Enygma_273

Enygma_274
(Lsr2-like DNA bridging protein)

Enygma_275

Enygma_276

Enygma_277

Enygma_278

Enygma_279
(ParB-like nuclease domain)

Enygma_280

Enygma_281

Enygma_282

Enygma_283

Enygma_284

Enygma_285

Enygma_286

Enygma_287

Enygma_288

Enygma_289

Enygma_290

Enygma_291

Enygma_292
(HNH endonuclease)
Submitted Annotation in DNA Master FormatDownload
Submitted Minimal DNA Master FileDownload
Author ListDownload
Annotation Cover SheetDownload
Publication Info
Uploaded to GenBank?Yes
GenBank AccessionPP358745
Refseq NumberNone yet
Archiving Info
Archiving status Archived
SEA Lysate Titer 1.44e12 pfu/mL
Pitt Freezer Box# 117
Pitt Freezer Box Grid# A3
Available Files
Plaque PictureDownload
Restriction Digest PictureDownload
EM PictureDownload
GenBank File for PhameratorDownload