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Streptomyces phage IchabodCrane
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Detailed Information for Phage IchabodCrane
Discovery Information
Isolation HostStreptomyces scabiei RL-34
Found ByPhoebe Mariano and Sydni Sobers
Year Found2018
Location FoundHanover, MD USA
Finding InstitutionUniversity of Maryland, Baltimore County
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?No
Isolation Temperature28°C
GPS Coordinates39.155679 N, 76.750807 W Map
Discovery NotesCollection date: September 11, 2019
Collection time: 12:20 p.m.

Temperature and Weather:
- 23.05 degrees Centigrade or 73.5 degrees Fahrenheit
- Foggy, cloudy with light showers 2 hours prior to collection
- Humidity: 94%

Geography: On a hill of a wooded community dug from 3.5 inches below

Soil Characteristics:
- moist, rich, blackish-brown
- surrounding tiny and large roots
Naming NotesIchabodCrane is a Siphoviridae with a distinguishable long and flexible tail. Capturing intact phage for imaging was complicated because its lengthy tail and capsid were severed throughout the sample. The slaughtered scenes were reminiscent of The Sleepy Hollow tale of lanky and headless Ichabod Crane. The time and wooded location in which the phage was discovered also befitted the Halloween season.
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedJan 25, 2019
Sequencing FacilityNC State Genomic Sciences Laboratory
Shotgun Sequencing MethodIllumina Sequencing
Approximate Shotgun Coverage1308
Genome length (bp)130730
Character of genome endsDirect Terminal Repeat
Direct Terminal Repeat Length12187 bp
GC Content49.4%
Fasta file available?Yes: Download fasta file
Characterization
ClusterBE
SubclusterBE2
Cluster Life CycleLytic
Lysogeny NotesLysogen tests were observed over 10 days. Mesa were not found. The tests were repeated twice to ensure that the lack of mesa was not due to other factors. Mesa were still not found which strongly suggests that IchabodCrane is a virulent bacteriophage for S.scabiei.
Other Cluster Members
Annotating InstitutionUniversity of Maryland, Baltimore County
Annotation StatusBeing Annotated (Expected completion by 8/31/2019)
Plaque Notes- Quickly-formed in 20 hours

- Clear, distinct, symmetric circles

- Plaque clusters will form due to phage traversing the agar and infecting nearby cells. It appears as if some plaques are larger than individual ones but they are usually two plaques infecting bacterium close in proximity.

- Plaque assay (at 20 hrs): 1.1 mm in diameter with clear edges under 28.4 degrees centigrade incubation

Note: When plaque assays have extended (28 C) incubation of 52 to 120 hours, or transferred to 4 C storage within 48 hours of incubation, the plaques will grow 1.9 mm in diameter with faint to cloudy edges.
MorphotypeSiphoviridae
Has been Phamerated?Yes
Gene List

Click to View

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Publication Info
Uploaded to GenBank?No
GenBank AccessionNone yet
Refseq NumberNone yet
Archiving Info
Archiving status Archived
Pitt Freezer Box# 83
Pitt Freezer Box Grid# G2
Available Files
Plaque PictureDownload
Restriction Digest PictureDownload
EM PictureDownload