| Detailed Information for Phage BoomerJR |
| Discovery Information |
| Isolation Host | Streptomyces lividans JI 1326 |
| Found By | Jessica Yavner and Sofia Kling |
| Year Found | 2017 |
| Location Found | St. Louis, MO USA |
| Finding Institution | Washington University in St. Louis |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | 35°C |
| GPS Coordinates | 38.640278 N, 90.294722 W Map |
| Discovery Notes | Sample F
Location: Forest Park - soil by row of trees across from art museum
Depth of dig: 3 cm
Soil Texture: wet and sandy
Outside Temperature: 95 degrees Fahrenheit
|
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Sep 20, 2018 |
| Sequencing Facility | The McDonnell Genome Institute at Washington University |
| Shotgun Sequencing Method | Illumina |
| Sequencer Used | Illumina MiSeq |
| Read Type | Single-end reads |
| Read Length | 150 bp |
| Approximate Shotgun Coverage | 72 |
| Genome length (bp) | 131220 |
| Character of genome ends | Direct Terminal Repeat |
| Direct Terminal Repeat Length | 12432 bp |
| GC Content | 49.2% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | BE |
| Subcluster | BE2 |
| Cluster Life Cycle | Lytic |
| Other Cluster Members |
|
| Annotating Institution | Washington University in St. Louis |
| Annotation Status | In GenBank |
| Plaque Notes | Bull's eye plaques
The plaques are clear with a big area of cloudiness around them
The clear plaques are ~0.1 cm in diameter and the cloudy area around the plaque + the clear plaque has a diameter of ~0.3 cm |
| Morphotype | Siphoviridae |
| Number of Genes | 242 |
| Number of tRNAs | 40 |
| Number of tmRNAs | 1 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Minimal DNA Master File | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | MK359351 |
| Refseq Number | None yet |
| Archiving Info |
| Archiving status |
Archived |
| SEA Lysate Titer |
3.2*10^10 |
| Pitt Freezer Box# |
49 |
| Pitt Freezer Box Grid# |
C4 |
| Available Files |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| EM Picture | Download |
| GenBank File for Phamerator | Download |
