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Streptomyces phage Bordeaux

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(S. griseus NBRC13350)

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Detailed Information for Phage Bordeaux
Discovery Information
Isolation Host	Streptomyces griseus ATCC 10137
Found By	Brendon Williams
Year Found	2018
Location Found	Flower Mound, TX USA
Finding Institution	University of North Texas
Program	Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?	Yes
Isolation Temperature	30°C
GPS Coordinates	33.041985 N, 97.172043 W Map
Discovery Notes	The soil sample was taken August 26th, 2018, at 3:55PM. The soil was largely dry with organic matter such as grass roots and other buried materials such as twigs and acorns, with very few rocks. The air temperature was 96 degrees F. The sample was taken from about 2 feet away from a tree, and consisted of dark and clumpy soil.
Naming Notes	The name Bordeaux comes from the street it was taken from, and also where I grew up. It reflects the temperate nature of the phage as just like the French wine town the street shares its name with, the phage took its time to reach a viable titer.
Sequencing Information
Sequencing Complete?	Yes
Date Sequencing Completed	Jan 8, 2019
Sequencing Facility	Pittsburgh Bacteriophage Institute
Shotgun Sequencing Method	Illumina
Sequencer Used	Illumina MiSeq
Read Type	Single-end reads
Read Length	150 bp
Approximate Shotgun Coverage	700
Genome length (bp)	131317
Character of genome ends	Direct Terminal Repeat
Direct Terminal Repeat Length	12579 bp
GC Content	49.5%
Fasta file available?	Yes: Download fasta file
Characterization
Cluster	BE
Subcluster	BE2
Cluster Life Cycle	Lytic
Lysogeny Notes	A spot test testing for lysogens had clear spots down to the 10^-7 dilution, lending evidence to a possibly negative conclusion.
Other Cluster Members	Click to View Anedea (BE1) Angela (BE1) Bartholomune (BE1) BlueOtter (BE1) Bmoc (BE1) Braelyn (BE1) Cadmus (BE1) Coogler (BE1) Cross (BE1) Cursive (BE1) Daubenski (BE1) Davielle (BE1) EGole (BE1) Evy (BE1) HangryHippo (BE1) Jay2Jay (BE1) Larnav (BE1) Leo04 (BE1) Liandry (BE1) LilMartin (BE1) Lululemon (BE1) Marsus (BE1) Mildred21 (BE1) MulchMansion (BE1) Navo (BE1) NootNoot (BE1) PacManQ (BE1) Paradiddles (BE1) Peebs (BE1) Pepperwood (BE1) Persimmon (BE1) PinkiePie (BE1) Riptide (BE1) Samisti12 (BE1) Scheme (BE1) Shuckle (BE1) Squillium (BE1) Sushi23 (BE1) Targaryen (BE1) Teutsch (BE1) Tribute (BE1) Warpy (BE1) Watermoore (BE1) WhereRU (BE1) Amabiko (BE2) AngryGiraffe (BE2) Battuta (BE2) Birchlyn (BE2) BoomerJR (BE2) Bordeaux (BE2) CeilingFan (BE2) Elmer (BE2) Enygma (BE2) Genie2 (BE2) Gibbi (BE2) IchabodCrane (BE2) JimJam (BE2) Jollison (BE2) Karimac (BE2) KentuckyRacer (BE2) LukeCage (BE2) MindFlayer (BE2) Mugiwara (BE2) PumpkinSpice (BE2) Quaran19 (BE2) Rikishi (BE2) SaltySpitoon (BE2) Sollertia (BE2) Spelly (BE2) Spilled (BE2) Stanimal (BE2) Starbow (BE2) StarPlatinum (BE2) Tomas (BE2) TomSawyer (BE2) Wipeout (BE2) Wofford (BE2) Yaboi (BE2)
Annotating Institution	University of North Texas
Annotation Status	In GenBank
Plaque Notes	Plaques were typically very small and cloudy. They were typically an average of about 2 mm across.
Number of Genes	238
Number of tRNAs	44
Number of tmRNAs	1
Has been Phamerated?	Yes
Gene List	Click to View Bordeaux_1 Bordeaux_2 Bordeaux_3 Bordeaux_4 Bordeaux_5 Bordeaux_6 Bordeaux_7 (Lsr2-like DNA bridging protein) Bordeaux_8 Bordeaux_9 Bordeaux_10 Bordeaux_11 Bordeaux_12 Bordeaux_13 (ParB-like nuclease domain protein) Bordeaux_14 Bordeaux_15 Bordeaux_16 Bordeaux_17 Bordeaux_18 Bordeaux_19 Bordeaux_20 Bordeaux_21 Bordeaux_22 Bordeaux_23 Bordeaux_24 Bordeaux_25 Bordeaux_26 (HNH endonuclease) Bordeaux_27 Bordeaux_28 (ThyX-like thymidylate synthase) Bordeaux_29 Bordeaux_30 Bordeaux_31 Bordeaux_32 Bordeaux_33 Bordeaux_34 Bordeaux_35 Bordeaux_36 Bordeaux_37 — tRNA (codon recognized: ACG) Bordeaux_38 (HNH endonuclease) Bordeaux_39 (endolysin) Bordeaux_40 Bordeaux_41 Bordeaux_42 (glycosyltransferase) Bordeaux_43 (ribonucleotide reductase) Bordeaux_44 (LysM-like peptidoglycan binding protein) Bordeaux_45 Bordeaux_46 Bordeaux_47 (portal protein) Bordeaux_48 Bordeaux_49 (capsid maturation protease) Bordeaux_50 (major capsid protein) Bordeaux_51 Bordeaux_52 Bordeaux_53 Bordeaux_54 Bordeaux_55 Bordeaux_56 (major tail protein) Bordeaux_57 (tail assembly chaperone) Bordeaux_58 Bordeaux_59 (tape measure protein) Bordeaux_60 Bordeaux_61 (minor tail protein) Bordeaux_62 Bordeaux_63 (minor tail protein) Bordeaux_64 (minor tail protein) Bordeaux_65 Bordeaux_66 Bordeaux_67 Bordeaux_68 (HNH endonuclease) Bordeaux_69 (metallophosphoesterase) Bordeaux_70 Bordeaux_71 Bordeaux_72 Bordeaux_73 Bordeaux_74 Bordeaux_75 Bordeaux_76 (DNA helicase) Bordeaux_77 Bordeaux_78 (DNA primase) Bordeaux_79 (DNA binding protein) Bordeaux_80 (DnaE-like DNA polymerase III alpha) Bordeaux_81 Bordeaux_82 (MazG-like nucleotide pyrophosphohydrolase) Bordeaux_83 (RecA-like DNA recombinase) Bordeaux_84 Bordeaux_85 (Holliday junction resolvase) Bordeaux_86 Bordeaux_87 Bordeaux_88 Bordeaux_89 (Cas4 family exonuclease) Bordeaux_90 Bordeaux_91 (RuvC-like resolvase) Bordeaux_92 (DprA-like ssDNA binding protein) Bordeaux_93 Bordeaux_94 (methyltransferase) Bordeaux_95 (thymidylate kinase) Bordeaux_96 Bordeaux_97 Bordeaux_98 (terminase) Bordeaux_99 (HNH endonuclease) Bordeaux_100 Bordeaux_101 — tRNA (codon recognized: GGA) Bordeaux_102 — tRNA (codon recognized: CAG) Bordeaux_103 — tRNA (codon recognized: UGG) Bordeaux_104 Bordeaux_105 Bordeaux_106 — tRNA (codon recognized: CCA) Bordeaux_107 — tRNA (codon recognized: CCA) Bordeaux_108 (nucleotidyltransferase) Bordeaux_109 (FtsK-like DNA translocase) Bordeaux_110 Bordeaux_111 — tRNA (codon recognized: AUA) Bordeaux_112 Bordeaux_113 — tRNA (codon recognized: UUA) Bordeaux_114 Bordeaux_115 Bordeaux_116 — tRNA (codon recognized: UCC) Bordeaux_117 Bordeaux_118 — tRNA (codon recognized: UCA) Bordeaux_119 Bordeaux_120 Bordeaux_121 Bordeaux_122 Bordeaux_123 Bordeaux_124 — tRNA (codon recognized: GAC) Bordeaux_125 Bordeaux_126 Bordeaux_127 — tRNA (codon recognized: GAC) Bordeaux_128 Bordeaux_129 (Ro-like RNA binding protein) Bordeaux_130 Bordeaux_131 Bordeaux_132 Bordeaux_133 Bordeaux_134 (DNA binding protein) Bordeaux_135 Bordeaux_136 Bordeaux_137 Bordeaux_138 Bordeaux_139 Bordeaux_140 — tRNA (codon recognized: GAG) Bordeaux_141 Bordeaux_142 — tRNA (codon recognized: GAA) Bordeaux_143 — tRNA (codon recognized: GUA) Bordeaux_144 — tRNA (codon recognized: CUA) Bordeaux_145 — tRNA (codon recognized: CUA) Bordeaux_146 — tRNA (codon recognized: UUC) Bordeaux_147 — tRNA (codon recognized: GGC) Bordeaux_148 — tRNA (codon recognized: AGA) Bordeaux_149 Bordeaux_150 Bordeaux_151 Bordeaux_152 — tRNA (codon recognized: GCA) Bordeaux_153 — tRNA (codon recognized: AAG) Bordeaux_154 (HNH endonuclease) Bordeaux_155 (peptidase) Bordeaux_156 Bordeaux_157 — tRNA (codon recognized: AAC) Bordeaux_158 Bordeaux_159 Bordeaux_160 — tRNA (codon recognized: AAC) Bordeaux_161 (band-7-like membrane protein) Bordeaux_162 Bordeaux_163 Bordeaux_164 — tRNA (codon recognized: AGG) Bordeaux_165 — tRNA (codon recognized: GCC) Bordeaux_166 — tRNA (codon recognized: AAG) Bordeaux_167 — tRNA (codon recognized: AAG) Bordeaux_168 Bordeaux_169 — tRNA (codon recognized: AAA) Bordeaux_170 Bordeaux_171 — tRNA (codon recognized: CGA) Bordeaux_172 Bordeaux_173 — tRNA (codon recognized: AUG) Bordeaux_174 — tRNA (codon recognized: AUG) Bordeaux_175 Bordeaux_176 Bordeaux_177 — tRNA (codon recognized: UAC) Bordeaux_178 — tRNA (codon recognized: UAC) Bordeaux_179 — tRNA (codon recognized: CAC) Bordeaux_180 Bordeaux_181 Bordeaux_182 Bordeaux_183 — tRNA (codon recognized: UGC) Bordeaux_184 — tRNA (codon recognized: ACA) Bordeaux_185 — tRNA (codon recognized: ACC) Bordeaux_186 Bordeaux_187 — tRNA (codon recognized: CGU) Bordeaux_188 Bordeaux_189 (DnaQ-like DNA polymerase III subunit) Bordeaux_190 Bordeaux_191 — tmRNA Bordeaux_192 Bordeaux_193 Bordeaux_194 (acetyltransferase) Bordeaux_195 Bordeaux_196 Bordeaux_197 Bordeaux_198 Bordeaux_199 Bordeaux_200 Bordeaux_201 Bordeaux_202 (RNA ligase) Bordeaux_203 Bordeaux_204 Bordeaux_205 Bordeaux_206 (polynucleotide kinase) Bordeaux_207 Bordeaux_208 Bordeaux_209 Bordeaux_210 — tRNA (codon recognized: CUC) Bordeaux_211 Bordeaux_212 Bordeaux_213 — tRNA (codon recognized: GUC) Bordeaux_214 (SprT-like protease) Bordeaux_215 Bordeaux_216 Bordeaux_217 Bordeaux_218 — tRNA (codon recognized: UUG) Bordeaux_219 Bordeaux_220 — tRNA (codon recognized: AGC) Bordeaux_221 Bordeaux_222 Bordeaux_223 Bordeaux_224 Bordeaux_225 Bordeaux_226 Bordeaux_227 Bordeaux_228 Bordeaux_229 (deoxycytidylate deaminase) Bordeaux_230 (phosphoesterase) Bordeaux_231 Bordeaux_232 Bordeaux_233 (WhiB family transcription factor) Bordeaux_234 Bordeaux_235 Bordeaux_236 Bordeaux_237 Bordeaux_238 Bordeaux_239 Bordeaux_240 Bordeaux_241 Bordeaux_242 Bordeaux_243 — tRNA (codon recognized: CAA) Bordeaux_244 Bordeaux_245 Bordeaux_246 Bordeaux_247 Bordeaux_248 Bordeaux_249 Bordeaux_250 (thioredoxin) Bordeaux_251 Bordeaux_252 Bordeaux_253 Bordeaux_254 Bordeaux_255 Bordeaux_256 Bordeaux_257 Bordeaux_258 Bordeaux_259 Bordeaux_260 Bordeaux_261 Bordeaux_262 Bordeaux_263 Bordeaux_264 (Lsr2-like DNA bridging protein) Bordeaux_265 Bordeaux_266 Bordeaux_267 Bordeaux_268 Bordeaux_269 Bordeaux_270 (ParB-like nuclease domain protein) Bordeaux_271 Bordeaux_272 Bordeaux_273 Bordeaux_274 Bordeaux_275 Bordeaux_276 Bordeaux_277 Bordeaux_278 Bordeaux_279 Bordeaux_280 Bordeaux_281 Bordeaux_282 Bordeaux_283 (HNH endonuclease)
Submitted Minimal DNA Master File	Download
Publication Info
Uploaded to GenBank?	Yes
GenBank Accession	MN369757
Refseq Number	None yet
Archiving Info
Archiving status	Archived
SEA Lysate Titer	4•10^7
Pitt Freezer Box#	75
Pitt Freezer Box Grid#	A9
Available Files
Plaque Picture	Download
Restriction Digest Picture	Download
EM Picture	Download
GenBank File for Phamerator	Download

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