| Detailed Information for Phage BruceLethal |
| Discovery Information |
| Isolation Host | Propionibacterium acnes ATCC 6919 |
| Found By | Benjamin Nguyen |
| Year Found | 2014 |
| Location Found | Los Angeles, CA USA |
| Finding Institution | University of California, Los Angeles |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | No |
| Isolation Temperature | 37°C |
| GPS Coordinates | 34.065 N, 118.4425 W Map |
| Discovery Notes | Phage isolation (from nose skin microcomedones) and genome annotation performed by Juan Carlos Espinosa, Benjamin Nguyen, Sean Perez, and Michelle Ramirez (UCLA). DNA preparation prepared by Krisanavane Reddi (UCLA). Assembly and quality control of annotation results performed by Jordan Moberg Parker and genome annotation curation performed by Dallas Mould (UCLA). Assembly confirmed by Charles Bowman (University of Pittsburgh). |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Apr 24, 2015 |
| Sequencing Facility | The UCLA Genotyping and Sequencing Core |
| Shotgun Sequencing Method | 454 Pyrosequencing |
| Approximate Shotgun Coverage | 62 |
| Genome length (bp) | 29249 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 11 bases |
| Overhang Sequence | TCGTACGGCTT |
| GC Content | 54.0% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | BU |
| Subcluster | -- |
| Cluster Life Cycle | Lytic |
| Other Cluster Members |
|
| Annotating Institution | Unknown or unassigned |
| Annotation Status | In GenBank |
| Plaque Notes | Round with turbid centers; average plaque size 3 mm in diameter (ranging in size from 2-4 mm) |
| Morphotype | Siphoviridae |
| Number of Genes | 44 |
| Number of tRNAs | 0 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | KR347352 |
| Refseq Number | None yet |
| Archiving Info |
| Archiving status |
Archived |
| Pitt Freezer Box# |
23 |
| Pitt Freezer Box Grid# |
D2 |
| Available Files |
| Plaque Picture | Download |
| EM Picture | Download |
| Final DNAMaster File | Download |
