| Detailed Information for Phage Cashline | |
| Discovery Information | |
| Isolation Host | Gordonia terrae 3612 |
| Found By | Colin Bashline |
| Year Found | 2019 |
| Location Found | Pittsburgh, PA United States of America |
| Finding Institution | University of Pittsburgh |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | No |
| Isolation Temperature | 26°C |
| GPS Coordinates | 40.4448 N, 79.9606 W Map |
| Discovery Notes | The site of soil collection was the mulchy area right outside Irvis Hall, along the pathway leading to the entrance of the Peterson Sports Complex. Nearby vegetation is the planted trees. The topsoil appears dark and dry. The weather was temperate, 80 degrees Fahrenheit and sunny. The soil sample was taken approximately one inch below topsoil. |
| Naming Notes | Phage was named from an anagram of the first letter of my (the discoverer's) first name replacing the first letter of my last name, a nickname I have had for years. |
| Sequencing Information | |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Mar 10, 2020 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina |
| Sequencer Used | Illumina MiSeq |
| Read Type | Single-end reads |
| Read Length | 150 bp |
| Approximate Shotgun Coverage | 2164 |
| Genome length (bp) | 52378 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 10 bases |
| Overhang Sequence | CGTATGGCAT |
| GC Content | 66.3% |
| Fasta file available? | Yes: Download fasta file |
| Characterization | |
| Cluster | CY |
| Subcluster | -- |
| Cluster Life Cycle | Temperate |
| Other Cluster Members | |
| Annotating Institution | University of Pittsburgh |
| Annotation Status | In GenBank |
| Plaque Notes | Plaques are relatively small, around 2mm in average diameter. Plaques are entirely clear and relatively regular in roundness. |
| Morphotype | Siphoviridae |
| Number of Genes | 76 |
| Number of tRNAs | 0 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
Click to ViewCashline_1 Cashline_2 Cashline_3 Cashline_4 Cashline_5 Cashline_6 Cashline_7 Cashline_8 Cashline_9 Cashline_10 Cashline_11 Cashline_12 Cashline_13 Cashline_14 Cashline_15 Cashline_16 Cashline_17 Cashline_18 Cashline_19 Cashline_20 Cashline_21 Cashline_22 Cashline_23 Cashline_24 Cashline_25 Cashline_26 Cashline_27 Cashline_28 Cashline_29 Cashline_30 Cashline_31 Cashline_32 Cashline_33 Cashline_34 Cashline_35 Cashline_36 Cashline_37 Cashline_38 Cashline_39 Cashline_40 Cashline_41 Cashline_42 Cashline_43 Cashline_44 Cashline_45 Cashline_46 Cashline_47 Cashline_48 Cashline_49 Cashline_50 Cashline_51 Cashline_52 Cashline_53 Cashline_54 Cashline_55 Cashline_56 Cashline_57 Cashline_58 Cashline_59 Cashline_60 Cashline_61 Cashline_62 Cashline_63 Cashline_64 Cashline_65 Cashline_66 Cashline_67 Cashline_68 Cashline_69 Cashline_70 Cashline_71 Cashline_72 Cashline_73 Cashline_74 Cashline_75 Cashline_76 |
| Submitted Minimal DNA Master File | Download |
| Publication Info | |
| Uploaded to GenBank? | Yes |
| GenBank Accession | MW534378 |
| Refseq Number | None yet |
| Archiving Info | |
| Archiving status | Archived |
| Pitt Freezer Box# | 113 |
| Pitt Freezer Box Grid# | H8 |
| Available Files | |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| EM Picture | Download |
| GenBank File for Phamerator | Download |
