| Detailed Information for Phage Cashline |
| Discovery Information |
| Isolation Host | Gordonia terrae 3612 |
| Found By | Colin Bashline |
| Year Found | 2019 |
| Location Found | Pittsburgh, PA United States of America |
| Finding Institution | University of Pittsburgh |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | No |
| Isolation Temperature | 26°C |
| GPS Coordinates | 40.4448 N, 79.9606 W Map |
| Discovery Notes | The site of soil collection was the mulchy area right outside Irvis Hall, along the pathway leading to the entrance of the Peterson Sports Complex. Nearby vegetation is the planted trees. The topsoil appears dark and dry. The weather was temperate, 80 degrees Fahrenheit and sunny. The soil sample was taken approximately one inch below topsoil. |
| Naming Notes | Phage was named from an anagram of the first letter of my (the discoverer's) first name replacing the first letter of my last name, a nickname I have had for years. |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Mar 10, 2020 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina |
| Approximate Shotgun Coverage | 2164 |
| Genome length (bp) | 52378 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 10 bases |
| Overhang Sequence | CGTATGGCAT |
| GC Content | 66.3% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | CY |
| Subcluster | -- |
| Cluster Life Cycle | Temperate |
| Other Cluster Members |
|
| Annotating Institution | University of Pittsburgh |
| Annotation Status | In GenBank |
| Plaque Notes | Plaques are relatively small, around 2mm in average diameter. Plaques are entirely clear and relatively regular in roundness. |
| Morphotype | Siphoviridae |
| Number of Genes | 76 |
| Number of tRNAs | 0 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Minimal DNA Master File | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | MW534378 |
| Refseq Number | None yet |
| Archiving Info |
| Archiving status |
Archived |
| Pitt Freezer Box# |
113 |
| Pitt Freezer Box Grid# |
H8 |
| Available Files |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| EM Picture | Download |
| GenBank File for Phamerator | Download |
