| Detailed Information for Phage Cross |
| Discovery Information |
| Isolation Host | Streptomyces sanglieri UNT16F27A |
| Found By | Richard Cross |
| Year Found | 2019 |
| Location Found | Denton, TX United States |
| Finding Institution | University of North Texas |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | 30°C |
| GPS Coordinates | 33.20479 N, 97.157788 W Map |
| Discovery Notes | The date was August 26, 2019, at 8:30am. The temperature for the day was 81°C and the soil was soft, and dry. It was a lighter brown color. |
| Naming Notes | The name of my phage comes from my last name, which is cross. |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Dec 17, 2019 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina |
| Sequencer Used | Illumina MiSeq |
| Read Type | Single-end reads |
| Read Length | 150 bp |
| Approximate Shotgun Coverage | 892 |
| Genome length (bp) | 132807 |
| Character of genome ends | Direct Terminal Repeat |
| Direct Terminal Repeat Length | 11075 bp |
| GC Content | 50.1% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | BE |
| Subcluster | BE1 |
| Cluster Life Cycle | Lytic |
| Lysogeny Notes | Cross tested negative for Lysogeny. |
| Other Cluster Members |
|
| Annotating Institution | University of North Texas |
| Annotation Status | In GenBank |
| Plaque Notes | My plaque morphology is mostly cloudy and usually ranges from 0.5mm to 1mm in diameter. |
| Morphotype | Siphoviridae |
| Number of Genes | 227 |
| Number of tRNAs | 43 |
| Number of tmRNAs | 1 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Minimal DNA Master File | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | MW507136 |
| Refseq Number | None yet |
| Archiving Info |
| Archiving status |
Archived |
| SEA Lysate Titer |
6.0x10^8 pfu/ml |
| Pitt Freezer Box# |
104 |
| Pitt Freezer Box Grid# |
A8 |
| Available Files |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| EM Picture | Download |
| GenBank File for Phamerator | Download |
