| Detailed Information for Phage DrParker | |
| Discovery Information | |
| Isolation Host | Propionibacterium acnes ATCC 6919 |
| Found By | Lariska Theron |
| Year Found | 2016 |
| Location Found | Los Angeles, CA USA |
| Finding Institution | University of California, Los Angeles |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Unknown |
| Isolation Temperature | 37°C |
| GPS Coordinates | 34.068900 N, 118.445200 W Map |
| Discovery Notes | Microcomedones (blackheads) were extracted from the nose of Jordan Moberg Parker using a Biore Pore Strip on 04/11/2016. Sample was enriched in RCM media with P. acnes strain ATCC 6919 under anaerobic conditions at 37oC. Isolation was performed by Lariska Theron (Undergraduate Learning Assistant for MIMG 103AL-S16) and amplification and DNA extraction were performed by Dr. Kris Reddi. Annotation was performed by Team Pox-anne: Alexander Chassiakos, Sandy (XiaoYing) Liang, Ziqi Zhou. TA: Andrew Lund |
| Naming Notes | The phage was isolated from the face of Dr. Parker, the supervising instructor for the course. |
| Sequencing Information | |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Aug 26, 2016 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina Sequencing |
| Approximate Shotgun Coverage | 1656 |
| Genome length (bp) | 29742 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 11 bases |
| Overhang Sequence | TCGTACGGCTT |
| GC Content | 54.6% |
| Fasta file available? | Yes: Download fasta file |
| Characterization | |
| Cluster | BU |
| Subcluster | -- |
| Cluster Life Cycle | Lytic |
| Lysogeny Notes | No evidence for lysogeny/psuedolysogeny based on clear plaque morphology. |
| Other Cluster Members |
Click to ViewAquarius ATCC29399BC ATCC29399BT Attacne BruceLethal Cota DrParker Enochoraptor Enoki Keiki Kubed Lauchelly Leviosa LilBandit MEAK Moyashi MrAK Ouroboros P1.1 P100.1 P100A P100D P101A P104A P104B P105 P106A P106C P106I P106L P106M P107A P107C P108C P14.4 P9.1 PA6 PAD20 PAS50 Pirate Procrass1 QueenBey Rileysaurus SKKY Solid Stormborn Supernova Wizzo |
| Annotating Institution | Unknown or unassigned |
| Annotation Status | In GenBank |
| Plaque Notes | Plaques are clear and average 1.35 mm (SD=0.47) in diameter, ranging from 0.7-2.3 mm in diameter. |
| Morphotype | Siphoviridae |
| Number of Genes | 45 |
| Number of tRNAs | 0 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
Click to ViewDrParker_1 DrParker_2 DrParker_3 DrParker_4 DrParker_5 DrParker_6 DrParker_7 DrParker_8 DrParker_9 DrParker_10 DrParker_11 DrParker_12 DrParker_13 DrParker_14 DrParker_15 DrParker_16 DrParker_17 DrParker_18 DrParker_19 DrParker_20 DrParker_21 DrParker_22 DrParker_23 DrParker_24 DrParker_25 DrParker_26 DrParker_27 DrParker_28 DrParker_29 DrParker_30 DrParker_31 DrParker_32 DrParker_33 DrParker_34 DrParker_35 DrParker_36 DrParker_37 DrParker_38 DrParker_39 DrParker_40 DrParker_41 DrParker_42 DrParker_43 DrParker_44 DrParker_45 |
| Submitted Minimal DNA Master File | Download |
| Publication Info | |
| Uploaded to GenBank? | Yes |
| GenBank Accession | MF919505 |
| Refseq Number | None yet |
| Archiving Info | |
| Archiving status | Archived |
| Pitt Freezer Box# | 28 |
| Pitt Freezer Box Grid# | E4 |
| Available Files | |
| Plaque Picture | Download |
| EM Picture | Download |
| GenBank File for Phamerator | Download |