| Discovery Information |
| Isolation Host | Arthrobacter globiformis B-2979 |
| Found By | Mariyam Arshad |
| Year Found | 2021 |
| Location Found | Naperville, IL United States of America |
| Finding Institution | Benedictine University |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | No |
| Isolation Temperature | 22°C |
| GPS Coordinates | 41.747583 N, 88.096583 W Map |
| Discovery Notes | The soil was dry, so I dug at it using the tip of my shoe. As I dug, it had a sand like composure, but was also had a slightly wet feeling. The soil had little rocks and sticks along with some weeds or plants. As I collected two handfuls of it, my nail punctured the bag and I collected a little bit of the weed's root. |
| Naming Notes | The phage is named Eesa after my four year old (now five year old) cousin because, initially, there was one tiny plaque. The plaque reminded me of my cousin because he is also very tiny. |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Jan 18, 2022 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina |
| Approximate Shotgun Coverage | 1482 |
| Genome length (bp) | 39792 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 12 bases |
| Overhang Sequence | GAGTCGCCGGCA |
| GC Content | 67.1% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | AS |
| Subcluster | AS1 |
| Cluster Life Cycle | Temperate |
| Other Cluster Members |
|
| Annotating Institution | Benedictine University |
| Annotation Status | In GenBank |
| Plaque Notes | The plaque has a diameter of 1 mm. At first, it was fuzzy and faint which lead to the phage being lysogenic. So, the phage takes 2 to 3 days to become more visible. However, there may be a lytic mutation because, with time, the plaques became more clear and continued to grow. |
| Number of Genes | 65 |
| Number of tRNAs | 0 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Annotation in DNA Master Format | Download |
| Submitted Minimal DNA Master File | Download |
| Author List | Download |
| Annotation Cover Sheet | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | OP751152 |
| Refseq Number | None yet |
| Archiving Info |
| Archiving status |
Not in Pitt Archives |
| SEA Lysate Titer |
2.7 x 10^10 |
| Available Files |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| EM Picture | Download |
| GenBank File for Phamerator | Download |
