Arthrobacter phage LittleTokyo
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Detailed Information for Phage LittleTokyo
Discovery Information
Isolation HostArthrobacter globiformis B-2979
Found ByOscar Lim
Year Found2019
Location FoundLos Angeles, CA United States
Finding InstitutionUniversity of California, Los Angeles
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?Yes
Isolation Temperature30°C
GPS Coordinates34.05 N, 118.24 W Map
Discovery NotesArthrobacter phage LittleTokyo was isolated from soil by direct plating at 30°C. The sample was collected by Oscar Lim from the Little Tokyo district of Los Angeles, CA near a corner of a shopping district outdoors. LIttleTokyo was isolated, characterized, and annotated by Oscar Lim, Shirley Zhang, Emily Pham, Daniel Peters, Tim Chiang, Kyra Forsyth, Laura Knittig, Shile Zhang and Emily Sardina (UCLA).

Instructor: Dr. Amanda Freise
Teaching Assistants: Malcolm Phung, Andrew Kapinos
Undergraduate Assistants: Andrew Ly & Karina Haleblian
Naming NotesIt was named LittleTokyo because it was discovered in the Little Tokyo district of Los Angeles.
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedAug 21, 2019
Sequencing FacilityPittsburgh Bacteriophage Institute
Shotgun Sequencing MethodIllumina Sequencing
Approximate Shotgun Coverage182
Genome length (bp)38969
Character of genome ends3' Sticky Overhang
Overhang Length12 bases
Overhang SequenceGAGTTGCCGGCA
GC Content66.0%
Fasta file available?Yes: Download fasta file
Characterization
ClusterAS
SubclusterAS2
Cluster Life CycleTemperate
Lysogeny Notestyrosine integrase identified.
Other Cluster Members
Annotating InstitutionUniversity of California, Los Angeles
Annotation StatusQC Complete
Plaque NotesClear and turbid plaques.
Average diameter: 1.5 mm
MorphotypeSiphoviridae
Number of Genes73
Has been Phamerated?Yes
Gene List

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LittleTokyo_1
(terminase, large subunit (ATPase domain))

LittleTokyo_2
(terminase, small subunit)

LittleTokyo_3
(terminase, large subunit (nuclease domain))

LittleTokyo_4
(RNA binding protein)

LittleTokyo_5
(portal protein)

LittleTokyo_6
(capsid maturation protease)

LittleTokyo_7
(major capsid protein)

LittleTokyo_8

LittleTokyo_9
(head-to-tail adaptor)

LittleTokyo_10
(head-to-tail stopper)

LittleTokyo_11

LittleTokyo_12
(tail terminator)

LittleTokyo_13
(major tail protein)

LittleTokyo_14
(tail assembly chaperone)

LittleTokyo_15
(tail assembly chaperone)

LittleTokyo_16
(tape measure protein)

LittleTokyo_17
(minor tail protein)

LittleTokyo_18
(minor tail protein)

LittleTokyo_19
(minor tail protein)

LittleTokyo_20
(minor tail protein)

LittleTokyo_21
(membrane protein)

LittleTokyo_22
(membrane protein)

LittleTokyo_23
(lysin A)

LittleTokyo_24
(membrane protein)

LittleTokyo_25

LittleTokyo_26
(helix-turn-helix DNA binding domain)

LittleTokyo_27

LittleTokyo_28

LittleTokyo_29

LittleTokyo_30

LittleTokyo_31

LittleTokyo_32
(HNH endonuclease)

LittleTokyo_33
(helix-turn-helix DNA binding domain)

LittleTokyo_34

LittleTokyo_35

LittleTokyo_36
(tyrosine integrase)

LittleTokyo_37
(immunity repressor)

LittleTokyo_38
(helix-turn-helix DNA binding domain)

LittleTokyo_39
(excise)

LittleTokyo_40
(exonuclease)

LittleTokyo_41
(RecT-like ssDNA binding protein)

LittleTokyo_42
(helix-turn-helix DNA binding domain)

LittleTokyo_43
(RusA-like resolvase (endonuclease))

LittleTokyo_44
(helix-turn-helix DNA binding domain)

LittleTokyo_45
(DNA methylase)

LittleTokyo_46
(helix-turn-helix DNA binding domain)

LittleTokyo_47

LittleTokyo_48

LittleTokyo_49
(membrane protein)

LittleTokyo_50

LittleTokyo_51
(nucleoside deoxyribosyltransferase)

LittleTokyo_52
(ssDNA binding protein)

LittleTokyo_53
(nuclease)

LittleTokyo_54

LittleTokyo_55

LittleTokyo_56

LittleTokyo_57

LittleTokyo_58

LittleTokyo_59

LittleTokyo_60

LittleTokyo_61

LittleTokyo_62

LittleTokyo_63

LittleTokyo_64

LittleTokyo_65

LittleTokyo_66

LittleTokyo_67

LittleTokyo_68

LittleTokyo_69

LittleTokyo_70

LittleTokyo_71
(helix-turn-helix DNA binding domain)

LittleTokyo_72
(PAPS reductase-like domain)

LittleTokyo_73
(HNH endonuclease)

Submitted Minimal DNA Master FileDownload
Publication Info
Uploaded to GenBank?No
GenBank AccessionNone yet
Refseq NumberNone yet
Archiving Info
Archiving status Not in Pitt Archives
Available Files
Plaque PictureDownload
EM PictureDownload
GenBank File for PhameratorDownload