| Discovery Information |
| Isolation Host | Arthrobacter globiformis B-2979 |
| Found By | Ashna Siddiqui |
| Year Found | 2021 |
| Location Found | Bedford, NH United States |
| Finding Institution | University of New Hampshire at Manchester |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | 18°C |
| GPS Coordinates | 42.941631 N, 71.474407 W Map |
| Discovery Notes | The sample was collected at the Target Parking lot in Bedford, New Hampshire, in the United States. The soil texture was dry, being slightly wet from earlier rain. The sample location was located near the road, and the air temperature was 64 degrees Fahrenheit (~17.7778 degrees Celsius). |
| Naming Notes | The phage name "Jamun" is taken from an Indian fried sweet called "gulab jamun." It's one of my favorite desserts/snacks, and it is round in shape, reminding me of the circular plaque shape. Additionally, "jamun" by itself is an Indian fruit, otherwise known as black plum. |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Jan 25, 2022 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina |
| Sequencer Used | Illumina MiSeq |
| Read Type | Single-end reads |
| Read Length | 150 bp |
| Approximate Shotgun Coverage | 1042 |
| Genome length (bp) | 38821 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 12 bases |
| Overhang Sequence | GAGTTGCCGGGA |
| GC Content | 67.8% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | AS |
| Subcluster | AS1 |
| Cluster Life Cycle | Temperate |
| Other Cluster Members |
|
| Annotating Institution | University of New Hampshire at Manchester |
| Annotation Status | In GenBank |
| Plaque Notes | The plaque size was around 0.12 cm in diameter. It was clear, with no cloudy rings, and the shape was similar to that of a small, irregular circle (edges seemed to be slightly "fuzzy" in appearance). |
| Morphotype | Siphoviridae |
| Number of Genes | 63 |
| Number of tRNAs | 0 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Annotation in DNA Master Format | Download |
| Submitted Minimal DNA Master File | Download |
| Author List | Download |
| Annotation Cover Sheet | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | OP297550 |
| Refseq Number | None yet |
| Archiving Info |
| Archiving status |
Archived |
| SEA Lysate Titer |
8.91 x 10^9 pfu/ml |
| Pitt Freezer Box# |
135 |
| Pitt Freezer Box Grid# |
D12 |
| Available Files |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| EM Picture | Download |
| GenBank File for Phamerator | Download |
