| Detailed Information for Phage Eesa | |
| Discovery Information | |
| Isolation Host | Arthrobacter globiformis B-2979 |
| Found By | Mariyam Arshad |
| Year Found | 2021 |
| Location Found | Naperville, IL United States of America |
| Finding Institution | Benedictine University |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | No |
| Isolation Temperature | 22°C |
| GPS Coordinates | 41.747583 N, 88.096583 W Map |
| Discovery Notes | The soil was dry, so I dug at it using the tip of my shoe. As I dug, it had a sand like composure, but was also had a slightly wet feeling. The soil had little rocks and sticks along with some weeds or plants. As I collected two handfuls of it, my nail punctured the bag and I collected a little bit of the weed's root. |
| Naming Notes | The phage is named Eesa after my four year old (now five year old) cousin because, initially, there was one tiny plaque. The plaque reminded me of my cousin because he is also very tiny. |
| Sequencing Information | |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Jan 18, 2022 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina Sequencing |
| Approximate Shotgun Coverage | 1482 |
| Genome length (bp) | 39792 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 12 bases |
| Overhang Sequence | GAGTCGCCGGCA |
| GC Content | 67.1% |
| Fasta file available? | Yes: Download fasta file |
| Characterization | |
| Cluster | AS |
| Subcluster | AS1 |
| Cluster Life Cycle | Temperate |
| Other Cluster Members | |
| Annotating Institution | Benedictine University |
| Annotation Status | QC Complete |
| Plaque Notes | The plaque has a diameter of 1 mm. At first, it was fuzzy and faint which lead to the phage being lysogenic. So, the phage takes 2 to 3 days to become more visible. However, there may be a lytic mutation because, with time, the plaques became more clear and continued to grow. |
| Has been Phamerated? | Yes |
| Gene List |
Click to ViewEesa_Draft_1 Eesa_Draft_2 Eesa_Draft_3 Eesa_Draft_4 Eesa_Draft_5 Eesa_Draft_6 Eesa_Draft_7 Eesa_Draft_8 Eesa_Draft_9 Eesa_Draft_10 Eesa_Draft_11 Eesa_Draft_12 Eesa_Draft_13 Eesa_Draft_14 Eesa_Draft_15 Eesa_Draft_16 Eesa_Draft_17 Eesa_Draft_18 Eesa_Draft_19 Eesa_Draft_20 Eesa_Draft_21 Eesa_Draft_22 Eesa_Draft_23 Eesa_Draft_24 Eesa_Draft_25 Eesa_Draft_26 Eesa_Draft_27 Eesa_Draft_28 Eesa_Draft_29 Eesa_Draft_30 Eesa_Draft_31 Eesa_Draft_32 Eesa_Draft_33 Eesa_Draft_34 Eesa_Draft_35 Eesa_Draft_36 Eesa_Draft_37 Eesa_Draft_38 Eesa_Draft_39 Eesa_Draft_40 Eesa_Draft_41 Eesa_Draft_42 Eesa_Draft_43 Eesa_Draft_44 Eesa_Draft_45 Eesa_Draft_46 Eesa_Draft_47 Eesa_Draft_48 Eesa_Draft_49 Eesa_Draft_50 Eesa_Draft_51 Eesa_Draft_52 Eesa_Draft_53 Eesa_Draft_54 Eesa_Draft_55 Eesa_Draft_56 Eesa_Draft_57 Eesa_Draft_58 Eesa_Draft_59 Eesa_Draft_60 Eesa_Draft_61 Eesa_Draft_62 Eesa_Draft_63 Eesa_Draft_64 Eesa_Draft_65 Eesa_Draft_66 Eesa_Draft_67 Eesa_Draft_68 Eesa_Draft_69 Eesa_Draft_70 |
| Submitted Annotation in DNA Master Format | Download |
| Submitted Minimal DNA Master File | Download |
| Author List | Download |
| Annotation Cover Sheet | Download |
| Publication Info | |
| Uploaded to GenBank? | No |
| GenBank Accession | None yet |
| Refseq Number | None yet |
| Archiving Info | |
| Archiving status | Not in Pitt Archives |
| SEA Lysate Titer | 2.7 x 10^10 |
| Available Files | |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| EM Picture | Download |
| GenBank File for Phamerator | Download |
