Arthrobacter phage Eesa
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Detailed Information for Phage Eesa
Discovery Information
Isolation HostArthrobacter globiformis B-2979
Found ByMariyam Arshad
Year Found2021
Location FoundNaperville, IL United States of America
Finding InstitutionBenedictine University
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?No
Isolation Temperature22°C
GPS Coordinates41.747583 N, 88.096583 W Map
Discovery NotesThe soil was dry, so I dug at it using the tip of my shoe. As I dug, it had a sand like composure, but was also had a slightly wet feeling. The soil had little rocks and sticks along with some weeds or plants. As I collected two handfuls of it, my nail punctured the bag and I collected a little bit of the weed's root.
Naming NotesThe phage is named Eesa after my four year old (now five year old) cousin because, initially, there was one tiny plaque. The plaque reminded me of my cousin because he is also very tiny.
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedJan 18, 2022
Sequencing FacilityPittsburgh Bacteriophage Institute
Shotgun Sequencing MethodIllumina Sequencing
Approximate Shotgun Coverage1482
Genome length (bp)39792
Character of genome ends3' Sticky Overhang
Overhang Length12 bases
Overhang SequenceGAGTCGCCGGCA
GC Content67.1%
Fasta file available?Yes: Download fasta file
Cluster Life CycleTemperate
Other Cluster Members
Annotating InstitutionBenedictine University
Annotation StatusIn GenBank
Plaque NotesThe plaque has a diameter of 1 mm. At first, it was fuzzy and faint which lead to the phage being lysogenic. So, the phage takes 2 to 3 days to become more visible. However, there may be a lytic mutation because, with time, the plaques became more clear and continued to grow.
Has been Phamerated?Yes
Gene List
Submitted Annotation in DNA Master FormatDownload
Submitted Minimal DNA Master FileDownload
Author ListDownload
Annotation Cover SheetDownload
Publication Info
Uploaded to GenBank?Yes
GenBank AccessionOP751152
Refseq NumberNone yet
Archiving Info
Archiving status Not in Pitt Archives
SEA Lysate Titer 2.7 x 10^10
Available Files
Plaque PictureDownload
Restriction Digest PictureDownload
EM PictureDownload
GenBank File for PhameratorDownload