| Detailed Information for Phage Eesa | |
| Discovery Information | |
| Isolation Host | Arthrobacter globiformis B-2979 |
| Found By | Mariyam Arshad |
| Year Found | 2021 |
| Location Found | Naperville, IL United States of America |
| Finding Institution | Benedictine University |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | No |
| Isolation Temperature | 22°C |
| GPS Coordinates | 41.747583 N, 88.096583 W Map |
| Discovery Notes | The soil was dry, so I dug at it using the tip of my shoe. As I dug, it had a sand like composure, but was also had a slightly wet feeling. The soil had little rocks and sticks along with some weeds or plants. As I collected two handfuls of it, my nail punctured the bag and I collected a little bit of the weed's root. |
| Naming Notes | The phage is named Eesa after my four year old (now five year old) cousin because, initially, there was one tiny plaque. The plaque reminded me of my cousin because he is also very tiny. |
| Sequencing Information | |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Jan 18, 2022 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina Sequencing |
| Approximate Shotgun Coverage | 1482 |
| Genome length (bp) | 39792 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 12 bases |
| Overhang Sequence | GAGTCGCCGGCA |
| GC Content | 67.1% |
| Fasta file available? | Yes: Download fasta file |
| Characterization | |
| Cluster | AS |
| Subcluster | AS1 |
| Cluster Life Cycle | Temperate |
| Other Cluster Members |
Click to ViewAbidatro (AS1) Basilisk (AS1) Brynnie (AS1) Chickaboom (AS1) Eesa (AS1) Galaxy (AS1) Jamun (AS1) Orcanus (AS1) Ruchi (AS1) TaylorSipht (AS1) Vulpecula (AS1) Amelia (AS2) Coral (AS2) Cote (AS2) Daob (AS2) HannahPhantana (AS2) Kepler (AS2) Kuleana (AS2) LittleTokyo (AS2) Lunar (AS2) Melons (AS2) Polka (AS2) Andrew (AS3) Juno112 (AS3) KHumphrey (AS3) Leona (AS3) PhluffyCoco (AS3) RedFox (AS3) Renna12 (AS3) |
| Annotating Institution | Benedictine University |
| Annotation Status | In GenBank |
| Plaque Notes | The plaque has a diameter of 1 mm. At first, it was fuzzy and faint which lead to the phage being lysogenic. So, the phage takes 2 to 3 days to become more visible. However, there may be a lytic mutation because, with time, the plaques became more clear and continued to grow. |
| Has been Phamerated? | Yes |
| Gene List |
Click to ViewEesa_1 Eesa_2 Eesa_3 Eesa_4 Eesa_5 Eesa_6 Eesa_7 Eesa_8 Eesa_9 Eesa_10 Eesa_11 Eesa_12 Eesa_13 Eesa_14 Eesa_15 Eesa_16 Eesa_17 Eesa_18 Eesa_19 Eesa_20 Eesa_21 Eesa_22 Eesa_23 Eesa_24 Eesa_25 Eesa_26 Eesa_27 Eesa_28 Eesa_29 Eesa_30 Eesa_31 Eesa_32 Eesa_33 Eesa_34 Eesa_35 Eesa_36 Eesa_37 Eesa_38 Eesa_39 Eesa_40 Eesa_41 Eesa_42 Eesa_43 Eesa_44 Eesa_45 Eesa_46 Eesa_47 Eesa_48 Eesa_49 Eesa_50 Eesa_51 Eesa_52 Eesa_53 Eesa_54 Eesa_55 Eesa_56 Eesa_57 Eesa_58 Eesa_59 Eesa_60 Eesa_61 Eesa_62 Eesa_63 Eesa_64 Eesa_65 |
| Submitted Annotation in DNA Master Format | Download |
| Submitted Minimal DNA Master File | Download |
| Author List | Download |
| Annotation Cover Sheet | Download |
| Publication Info | |
| Uploaded to GenBank? | Yes |
| GenBank Accession | OP751152 |
| Refseq Number | None yet |
| Archiving Info | |
| Archiving status | Not in Pitt Archives |
| SEA Lysate Titer | 2.7 x 10^10 |
| Available Files | |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| EM Picture | Download |
| GenBank File for Phamerator | Download |
