| Detailed Information for Phage Kuleana | |
| Discovery Information | |
| Isolation Host | Arthrobacter globiformis B-2979 |
| Found By | Kamalani Oshiro |
| Year Found | 2018 |
| Location Found | Honolulu, HI USA |
| Finding Institution | University of Hawaii at Manoa |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | No |
| Isolation Temperature | 23°C |
| GPS Coordinates | 21.2997 N, 157.8174 W Map |
| Discovery Notes | Found in wet soil next to a building and several plants in the shade of tamarind trees. Found along with several other phages in a direct isolation. We are confident that this phage is not the same as others found from the same collection because it has behaved very differently. Webbed plates were achieved quickly and the phage produced a large amount of DNA which was not the case for other phages from the same isolation. |
| Naming Notes | The name Kuleana comes from the Hawaiian word that translates to responsibility, ownership, interest and many more. The reason why I chose this name is because those three words describes what it takes to isolate and “care” for this phage. |
| Sequencing Information | |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Jan 31, 2019 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina Sequencing |
| Approximate Shotgun Coverage | 2531 |
| Genome length (bp) | 38850 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 12 bases |
| Overhang Sequence | GAGCTGCCGGCA |
| GC Content | 65.8% |
| Fasta file available? | Yes: Download fasta file |
| Characterization | |
| Cluster | AS |
| Subcluster | AS2 |
| Cluster Life Cycle | Temperate |
| Other Cluster Members | |
| Annotating Institution | University of Hawaii at Manoa |
| Annotation Status | In GenBank |
| Plaque Notes | Plaques were about 4 mm in diameter and appear cloudy. |
| Has been Phamerated? | Yes |
| Gene List |
Click to ViewKuleana_1 Kuleana_2 Kuleana_3 Kuleana_4 Kuleana_5 Kuleana_6 Kuleana_7 Kuleana_8 Kuleana_9 Kuleana_10 Kuleana_11 Kuleana_12 Kuleana_13 Kuleana_14 Kuleana_15 Kuleana_16 Kuleana_17 Kuleana_18 Kuleana_19 Kuleana_20 Kuleana_21 Kuleana_22 Kuleana_23 Kuleana_24 Kuleana_25 Kuleana_26 Kuleana_27 Kuleana_28 Kuleana_29 Kuleana_30 Kuleana_31 Kuleana_32 Kuleana_33 Kuleana_34 Kuleana_35 Kuleana_36 Kuleana_37 Kuleana_38 Kuleana_39 Kuleana_40 Kuleana_41 Kuleana_42 Kuleana_43 Kuleana_44 Kuleana_45 Kuleana_46 Kuleana_47 Kuleana_48 Kuleana_49 Kuleana_50 Kuleana_51 Kuleana_52 Kuleana_53 Kuleana_54 Kuleana_55 Kuleana_56 Kuleana_57 Kuleana_58 Kuleana_59 Kuleana_60 Kuleana_61 Kuleana_62 Kuleana_63 Kuleana_64 Kuleana_65 Kuleana_66 Kuleana_67 Kuleana_68 Kuleana_69 Kuleana_70 Kuleana_71 Kuleana_72 Kuleana_73 Kuleana_74 Kuleana_75 Kuleana_76 |
| Submitted Annotation in DNA Master Format | Download |
| Submitted Minimal DNA Master File | Download |
| Author List | Download |
| Annotation Cover Sheet | Download |
| Publication Info | |
| Uploaded to GenBank? | Yes |
| GenBank Accession | MN484600 |
| Refseq Number | None yet |
| Archiving Info | |
| Archiving status | Archived |
| Pitt Freezer Box# | 78 |
| Pitt Freezer Box Grid# | F3 |
| Available Files | |
| Plaque Picture | Download |
| Virtual Digest Picture | Download |
| EM Picture | Download |
| GenBank File for Phamerator | Download |
