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Arthrobacter phage Kuleana
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Detailed Information for Phage Kuleana
Discovery Information
Isolation HostArthrobacter globiformis B-2979
Found ByKamalani Oshiro
Year Found2018
Location FoundHonolulu , HI USA
Finding InstitutionUniversity of Hawaii at Manoa
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?No
Isolation Temperature23°C
GPS Coordinates21.2997 N, 157.8174 W Map
Discovery NotesFound in wet soil next to a building and several plants in the shade of tamarind trees. Found along with several other phages in a direct isolation. We are confident that this phage is not the same as others found from the same collection because it has behaved very differently. Webbed plates were achieved quickly and the phage produced a large amount of DNA which was not the case for other phages from the same isolation.
Naming NotesThe name Kuleana comes from the Hawaiian word that translates to responsibility, ownership, interest and many more. The reason why I chose this name is because those three words describes what it takes to isolate and “care” for this phage.
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedJan 31, 2019
Sequencing FacilityPittsburgh Bacteriophage Institute
Shotgun Sequencing MethodIllumina Sequencing
Approximate Shotgun Coverage2531
Genome length (bp)38850
Character of genome ends3' Sticky Overhang
Overhang Length12 bases
Overhang SequenceGAGCTGCCGGCA
GC Content65.8%
Fasta file available?Yes: Download fasta file
Characterization
ClusterAS
SubclusterAS2
Cluster Life CycleTemperate
Other Cluster Members
Annotation StatusOverdue (Expected completion by 5/15/2019)
Plaque NotesPlaques were about 4 mm in diameter and appear cloudy.
Has been Phamerated?Yes
Gene List
Publication Info
Uploaded to GenBank?No
GenBank AccessionNone yet
Refseq NumberNone yet
Archiving Info
Archiving status Archived
Pitt Freezer Box# 78
Pitt Freezer Box Grid# F3
Available Files
Plaque PictureDownload
Virtual Digest PictureDownload
EM PictureDownload