Arthrobacter phage Kuleana
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Detailed Information for Phage Kuleana
Discovery Information
Isolation HostArthrobacter globiformis B-2979
Found ByKamalani Oshiro
Year Found2018
Location FoundHonolulu, HI USA
Finding InstitutionUniversity of Hawaii at Manoa
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?No
Isolation Temperature23°C
GPS Coordinates21.2997 N, 157.8174 W Map
Discovery NotesFound in wet soil next to a building and several plants in the shade of tamarind trees. Found along with several other phages in a direct isolation. We are confident that this phage is not the same as others found from the same collection because it has behaved very differently. Webbed plates were achieved quickly and the phage produced a large amount of DNA which was not the case for other phages from the same isolation.
Naming NotesThe name Kuleana comes from the Hawaiian word that translates to responsibility, ownership, interest and many more. The reason why I chose this name is because those three words describes what it takes to isolate and “care” for this phage.
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedJan 31, 2019
Sequencing FacilityPittsburgh Bacteriophage Institute
Shotgun Sequencing MethodIllumina Sequencing
Approximate Shotgun Coverage2531
Genome length (bp)38850
Character of genome ends3' Sticky Overhang
Overhang Length12 bases
Overhang SequenceGAGCTGCCGGCA
GC Content65.8%
Fasta file available?Yes: Download fasta file
Characterization
ClusterAS
SubclusterAS2
Cluster Life CycleTemperate
Other Cluster Members
Annotating InstitutionUniversity of Hawaii at Manoa
Annotation StatusIn GenBank
Plaque NotesPlaques were about 4 mm in diameter and appear cloudy.
Has been Phamerated?Yes
Gene List

Click to View

Kuleana_1

Kuleana_2
(terminase, small subunit)

Kuleana_3
(terminase, large subunit)

Kuleana_4
(RNA binding protein)

Kuleana_5
(portal protein)

Kuleana_6
(capsid maturation protease)

Kuleana_7
(major capsid protein)

Kuleana_8

Kuleana_9
(head-to-tail adaptor)

Kuleana_10
(head-to-tail stopper)

Kuleana_11

Kuleana_12
(tail terminator)

Kuleana_13
(major tail protein)

Kuleana_14
(tail assembly chaperone)

Kuleana_15
(tail assembly chaperone)

Kuleana_16
(tape measure protein)

Kuleana_17
(minor tail protein)

Kuleana_18
(minor tail protein)

Kuleana_19
(minor tail protein)

Kuleana_20
(minor tail protein)

Kuleana_21
(membrane protein)

Kuleana_22

Kuleana_23
(lysin A)

Kuleana_24
(membrane protein)

Kuleana_25

Kuleana_26

Kuleana_27
(helix-turn-helix DNA binding domain)

Kuleana_28

Kuleana_29

Kuleana_30

Kuleana_31

Kuleana_32
(HNH endonuclease)

Kuleana_33

Kuleana_34

Kuleana_35

Kuleana_36

Kuleana_37
(tyrosine integrase)

Kuleana_38
(immunity repressor)

Kuleana_39
(helix-turn-helix DNA binding domain)

Kuleana_40
(excise)

Kuleana_41

Kuleana_42
(RecE-like exonuclease)

Kuleana_43
(RecT-like ssDNA binding protein)

Kuleana_44
(helix-turn-helix DNA binding domain)

Kuleana_45
(RusA-like resolvase (endonuclease))

Kuleana_46
(helix-turn-helix DNA binding domain)

Kuleana_47
(DNA methylase)

Kuleana_48

Kuleana_49

Kuleana_50

Kuleana_51

Kuleana_52
(membrane protein)

Kuleana_53

Kuleana_54
(nucleoside deoxyribosyltransferase)

Kuleana_55
(ssDNA binding protein)

Kuleana_56

Kuleana_57
(endonuclease)

Kuleana_58

Kuleana_59

Kuleana_60

Kuleana_61

Kuleana_62

Kuleana_63

Kuleana_64

Kuleana_65

Kuleana_66

Kuleana_67

Kuleana_68

Kuleana_69

Kuleana_70

Kuleana_71

Kuleana_72

Kuleana_73

Kuleana_74
(membrane protein)

Kuleana_75
(helix-turn-helix DNA binding domain)

Kuleana_76
(HNH endonuclease)

Submitted Annotation in DNA Master FormatDownload
Submitted Minimal DNA Master FileDownload
Author ListDownload
Annotation Cover SheetDownload
Publication Info
Uploaded to GenBank?Yes
GenBank AccessionMN484600
Refseq NumberNone yet
Archiving Info
Archiving status Archived
Pitt Freezer Box# 78
Pitt Freezer Box Grid# F3
Available Files
Plaque PictureDownload
Virtual Digest PictureDownload
EM PictureDownload
GenBank File for PhameratorDownload