| Detailed Information for Phage Kuleana |
| Discovery Information |
| Isolation Host | Arthrobacter globiformis B-2979 |
| Found By | Kamalani Oshiro |
| Year Found | 2018 |
| Location Found | Honolulu, HI USA |
| Finding Institution | University of Hawaii at Manoa |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | No |
| Isolation Temperature | 23°C |
| GPS Coordinates | 21.2997 N, 157.8174 W Map |
| Discovery Notes | Found in wet soil next to a building and several plants in the shade of tamarind trees. Found along with several other phages in a direct isolation. We are confident that this phage is not the same as others found from the same collection because it has behaved very differently. Webbed plates were achieved quickly and the phage produced a large amount of DNA which was not the case for other phages from the same isolation. |
| Naming Notes | The name Kuleana comes from the Hawaiian word that translates to responsibility, ownership, interest and many more. The reason why I chose this name is because those three words describes what it takes to isolate and “care” for this phage. |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Jan 31, 2019 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina |
| Sequencer Used | Illumina MiSeq |
| Read Type | Single-end reads |
| Read Length | 150 bp |
| Approximate Shotgun Coverage | 2531 |
| Genome length (bp) | 38850 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 12 bases |
| Overhang Sequence | GAGCTGCCGGCA |
| GC Content | 65.8% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | AS |
| Subcluster | AS2 |
| Cluster Life Cycle | Temperate |
| Other Cluster Members |
|
| Annotating Institution | University of Hawaii at Manoa |
| Annotation Status | In GenBank |
| Plaque Notes | Plaques were about 4 mm in diameter and appear cloudy. |
| Number of Genes | 76 |
| Number of tRNAs | 0 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Annotation in DNA Master Format | Download |
| Submitted Minimal DNA Master File | Download |
| Author List | Download |
| Annotation Cover Sheet | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | MN484600 |
| Refseq Number | None yet |
| Archiving Info |
| Archiving status |
Archived |
| Pitt Freezer Box# |
78 |
| Pitt Freezer Box Grid# |
F3 |
| Available Files |
| Plaque Picture | Download |
| Virtual Digest Picture | Download |
| EM Picture | Download |
| GenBank File for Phamerator | Download |
