| Detailed Information for Phage Fork |
| Discovery Information |
| Isolation Host | Microbacterium foliorum NRRL B-24224 |
| Found By | Kiley Jones & Olivia Heil |
| Year Found | 2017 |
| Location Found | River Falls, WI USA |
| Finding Institution | University of Wisconsin-River Falls |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | Not entered |
| GPS Coordinates | 44.850833 N, 92.619166 W Map |
| Discovery Notes | The soil was found near the South Fork River right on the river bank under a bridge. Almost near 6th St in River Falls, WI. It was a moist, wet condition when found. The weather was a temperature of 72 degrees and cloudy/rainy. |
| Naming Notes | We named this phage Fork from the river it was found in, "South Fork". |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Jan 10, 2018 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina |
| Approximate Shotgun Coverage | 2399 |
| Genome length (bp) | 62090 |
| Character of genome ends | Direct Terminal Repeat |
| Direct Terminal Repeat Length | 3099 bp |
| GC Content | 61.8% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | ED |
| Subcluster | ED2 |
| Cluster Life Cycle | Lytic |
| Other Cluster Members |
|
| Annotating Institution | University of Wisconsin-River Falls |
| Annotation Status | In GenBank |
| Plaque Notes | Fork was a visible plaque that was just a small hole on the agar plate. When first picking plaques there were three different shapes to differ from. Fork was picked from the soil extract plate and when the process was redone there was a uniform supply of visible plaques on the plate that was later named Fork. |
| Number of Genes | 117 |
| Number of tRNAs | 3 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Minimal DNA Master File | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | MH371108 |
| Refseq Number | None yet |
| Archiving Info |
| Archiving status |
Archived |
| Pitt Freezer Box# |
69 |
| Pitt Freezer Box Grid# |
F11 |
| Available Files |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| EM Picture | Download |
| GenBank File for Phamerator | Download |
