| Detailed Information for Phage Fork | |
| Discovery Information | |
| Isolation Host | Microbacterium foliorum NRRL B-24224 |
| Found By | Kiley Jones & Olivia Heil |
| Year Found | 2017 |
| Location Found | River Falls, WI USA |
| Finding Institution | University of Wisconsin-River Falls |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | Not entered |
| GPS Coordinates | 44.850833 N, 92.619166 W Map |
| Discovery Notes | The soil was found near the South Fork River right on the river bank under a bridge. Almost near 6th St in River Falls, WI. It was a moist, wet condition when found. The weather was a temperature of 72 degrees and cloudy/rainy. |
| Naming Notes | We named this phage Fork from the river it was found in, "South Fork". |
| Sequencing Information | |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Jan 10, 2018 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina Sequencing |
| Approximate Shotgun Coverage | 2399 |
| Genome length (bp) | 62090 |
| Character of genome ends | Direct Terminal Repeat |
| Direct Terminal Repeat Length | 3099 bp |
| GC Content | 61.8% |
| Fasta file available? | Yes: Download fasta file |
| Characterization | |
| Cluster | ED |
| Subcluster | ED2 |
| Cluster Life Cycle | Lytic |
| Other Cluster Members |
Click to ViewWolfstar (None) Alleb (ED1) DejaVu (ED1) Hortus1 (ED1) Hubbs (ED1) Jacko (ED1) Lupine (ED1) OlinDD (ED1) Pavlo (ED1) PhillyPhilly (ED1) Pioneer3 (ED1) Platte (ED1) Roman (ED1) Tandem (ED1) ASegato (ED2) DustyDino (ED2) Erenyeager (ED2) Fork (ED2) Lyell (ED2) Musetta (ED2) Necrophoxinus (ED2) RunningBrook (ED2) StevieWelch (ED2) Welcome (ED2) Yuma (ED2) |
| Annotating Institution | University of Wisconsin-River Falls |
| Annotation Status | In GenBank |
| Plaque Notes | Fork was a visible plaque that was just a small hole on the agar plate. When first picking plaques there were three different shapes to differ from. Fork was picked from the soil extract plate and when the process was redone there was a uniform supply of visible plaques on the plate that was later named Fork. |
| Number of Genes | 117 |
| Number of tRNAs | 3 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
Click to ViewFork_1 Fork_2 Fork_3 Fork_4 Fork_5 Fork_6 Fork_7 Fork_8 Fork_9 Fork_10 Fork_11 Fork_12 Fork_13 Fork_14 Fork_15 Fork_16 Fork_17 Fork_18 Fork_19 Fork_20 Fork_21 Fork_22 Fork_23 Fork_24 — tRNA Fork_25 — tRNA Fork_26 Fork_27 Fork_28 Fork_29 Fork_30 Fork_31 Fork_32 Fork_33 Fork_34 Fork_35 Fork_36 Fork_37 Fork_38 Fork_39 Fork_40 Fork_41 Fork_42 Fork_43 Fork_44 Fork_45 Fork_46 Fork_47 Fork_48 Fork_49 Fork_50 Fork_51 Fork_52 Fork_53 Fork_54 Fork_55 Fork_56 Fork_57 Fork_58 Fork_59 Fork_60 Fork_61 Fork_62 Fork_63 Fork_64 Fork_65 Fork_66 Fork_120 — tRNA Fork_67 Fork_68 Fork_69 Fork_70 Fork_71 Fork_72 Fork_73 Fork_74 Fork_75 Fork_76 Fork_77 Fork_78 Fork_79 Fork_80 Fork_81 Fork_82 Fork_83 Fork_84 Fork_85 Fork_86 Fork_87 Fork_88 Fork_89 Fork_90 Fork_91 Fork_92 Fork_93 Fork_94 Fork_95 Fork_96 Fork_97 Fork_98 Fork_99 Fork_100 Fork_101 Fork_102 Fork_103 Fork_104 Fork_105 Fork_106 Fork_107 Fork_108 Fork_109 Fork_110 Fork_111 Fork_112 Fork_113 Fork_114 Fork_115 Fork_116 Fork_117 Fork_118 Fork_119 |
| Submitted Minimal DNA Master File | Download |
| Publication Info | |
| Uploaded to GenBank? | Yes |
| GenBank Accession | MH371108 |
| Refseq Number | None yet |
| Archiving Info | |
| Archiving status | Archived |
| Pitt Freezer Box# | 69 |
| Pitt Freezer Box Grid# | F11 |
| Available Files | |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| EM Picture | Download |
| GenBank File for Phamerator | Download |
