| Detailed Information for Phage Genie2 |
| Discovery Information |
| Isolation Host | Streptomyces lividans JI 1326 |
| Found By | Katy Mockett and Adeshola Fanegan |
| Year Found | 2017 |
| Location Found | St. Louis, MO USA |
| Finding Institution | Washington University in St. Louis |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | No |
| Isolation Temperature | 20°C |
| GPS Coordinates | 38.646276 N, 90.310504 W Map |
| Discovery Notes | Gathered September 5th
Lots of nearby organic material- dead leaves, flowers, mulch |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Sep 20, 2018 |
| Sequencing Facility | The McDonnell Genome Institute at Washington University |
| Shotgun Sequencing Method | Illumina |
| Sequencer Used | Illumina MiSeq |
| Read Type | Single-end reads |
| Read Length | 150 bp |
| Approximate Shotgun Coverage | 82 |
| Genome length (bp) | 131333 |
| Character of genome ends | Direct Terminal Repeat |
| Direct Terminal Repeat Length | 12432 bp |
| GC Content | 49.3% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | BE |
| Subcluster | BE2 |
| Cluster Life Cycle | Lytic |
| Other Cluster Members |
|
| Annotating Institution | Washington University in St. Louis |
| Annotation Status | In GenBank |
| Plaque Notes | Plates demonstrated crowding.
For uncrowded plates, plaques were approximately 3mm in diameter with a dense clearing in the middle approximately 1mm in diameter surrounded by a hazy ring.
Plaques were circular in shape and had smooth edges. |
| Morphotype | Siphoviridae |
| Number of Genes | 242 |
| Number of tRNAs | 40 |
| Number of tmRNAs | 1 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Minimal DNA Master File | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | MK359332 |
| Refseq Number | None yet |
| Archiving Info |
| Archiving status |
Archived |
| SEA Lysate Titer |
2.44 * 10^10 pfu/mL |
| Pitt Freezer Box# |
49 |
| Pitt Freezer Box Grid# |
C7 |
| Available Files |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| EM Picture | Download |
| GenBank File for Phamerator | Download |
