| Discovery Information |
| Isolation Host | Streptomyces scabiei RL-34 |
| Found By | Phoebe Mariano and Sydni Sobers |
| Year Found | 2018 |
| Location Found | Hanover, MD USA |
| Finding Institution | University of Maryland, Baltimore County |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | No |
| Isolation Temperature | 28°C |
| GPS Coordinates | 39.155679 N, 76.750807 W Map |
| Discovery Notes | Collection date: September 11, 2018
Collection time: 12:20 p.m.
Temperature and Weather:
- 23.05 degrees Centigrade or 73.5 degrees Fahrenheit
- Foggy, cloudy with light showers 2 hours prior to collection
- Humidity: 94%
Geography: On a hill of a wooded community dug from 3.5 inches below
Soil Characteristics:
- moist, rich, blackish-brown
- surrounding tiny and large roots |
| Naming Notes | IchabodCrane is a Siphoviridae with a distinguishable long and flexible tail. Capturing intact phage for imaging was complicated because its lengthy tail and capsid were severed throughout the sample. The slaughtered scenes were reminiscent of The Sleepy Hollow tale of lanky and headless Ichabod Crane. The time and wooded location in which the phage was discovered also befitted the Halloween season. |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Jan 25, 2019 |
| Sequencing Facility | NC State Genomic Sciences Laboratory |
| Shotgun Sequencing Method | Illumina Sequencing |
| Approximate Shotgun Coverage | 1308 |
| Genome length (bp) | 130730 |
| Character of genome ends | Direct Terminal Repeat |
| Direct Terminal Repeat Length | 12187 bp |
| GC Content | 49.4% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | BE |
| Subcluster | BE2 |
| Cluster Life Cycle | Lytic |
| Lysogeny Notes | Lysogen tests were observed over 10 days. Mesa were not found. The tests were repeated twice to ensure that the lack of mesa was not due to other factors. Mesa were still not found which strongly suggests that IchabodCrane is a virulent bacteriophage for S.scabiei. |
| Other Cluster Members |
|
| Annotating Institution | University of Maryland, Baltimore County |
| Annotation Status | In GenBank |
| Plaque Notes | - Quickly-formed in 20 hours
- Clear, distinct, symmetric circles
- Plaque clusters will form due to phage traversing the agar and infecting nearby cells. It appears as if some plaques are larger than individual ones but they are usually two plaques infecting bacterium close in proximity.
- Plaque assay (at 20 hrs): 1.1 mm in diameter with clear edges under 28.4 degrees centigrade incubation
Note: When plaque assays have extended (28 C) incubation of 52 to 120 hours, or transferred to 4 C storage within 48 hours of incubation, the plaques will grow 1.9 mm in diameter with faint to cloudy edges. |
| Morphotype | Siphoviridae |
| Number of Genes | 239 |
| Number of tRNAs | 39 |
| Number of tmRNAs | 1 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Minimal DNA Master File | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | MN428060 |
| Refseq Number | None yet |
| Archiving Info |
| Archiving status |
Archived |
| Pitt Freezer Box# |
83 |
| Pitt Freezer Box Grid# |
G2 |
| Available Files |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| EM Picture | Download |
| GenBank File for Phamerator | Download |
