Microbacterium phage Jacko
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Detailed Information for Phage Jacko
Discovery Information
Isolation HostMicrobacterium paraoxydans NRRL B-14843
Found ByJon Duclos and Rudy Lipovetz
Year Found2017
Location FoundHudson, WI USA
Finding InstitutionUniversity of Wisconsin-River Falls
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?Yes
Isolation Temperature28°C
GPS Coordinates44.984533 N, 92.7545 W Map
Discovery NotesSoil found in Dr. Klyczek's compost pile, sample came from bottom of pile. Compost was well broken down. Soil was semi-moist, loose and contained partially decomposed leaves. Compost contained yard waste and vegetable scraps.
Naming NotesThe name "Jacko" is short for jack-o-lantern. It is also a character in a story.
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedMar 8, 2018
Sequencing FacilityPittsburgh Bacteriophage Institute
Shotgun Sequencing MethodIllumina Sequencing
Genome length (bp)61421
Character of genome endsUnknown
Overhang Length3028 bases
GC Content64.9%
Fasta file available?Yes: Download fasta file
Characterization
ClusterED
SubclusterED1
Cluster Life CycleLytic
Other Cluster Members
Annotating InstitutionUniversity of Wisconsin-River Falls
Annotation StatusIn GenBank
Plaque Notesmedium to large clear plaques, no halo
MorphotypeSiphoviridae
Number of Genes114
Number of tRNAs2
Number of tmRNAs0
Has been Phamerated?Yes
Gene List

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Jacko_1

Jacko_2

Jacko_3

Jacko_4

Jacko_5

Jacko_6

Jacko_7

Jacko_8

Jacko_9

Jacko_10

Jacko_11

Jacko_12

Jacko_13

Jacko_14

Jacko_15

Jacko_16

Jacko_17

Jacko_18

Jacko_19

Jacko_20

Jacko_21

Jacko_22

Jacko_23 — tRNA
(tRNA-Asn(gtt))

Jacko_24 — tRNA
(tRNA-Thr(cgt))

Jacko_25

Jacko_26

Jacko_27

Jacko_28

Jacko_29

Jacko_30

Jacko_31
(NrdH-like glutaredoxin)

Jacko_33
(exonuclease)

Jacko_34

Jacko_35
(terminase)

Jacko_36
(portal protein)

Jacko_37
(capsid maturation protease)

Jacko_38

Jacko_39
(major capsid protein)

Jacko_40

Jacko_41

Jacko_42
(head-to-tail stopper)

Jacko_43

Jacko_44
(tail terminator)

Jacko_45

Jacko_46

Jacko_47

Jacko_48
(tail assembly chaperone)

Jacko_49
(tail assembly chaperone)

Jacko_50
(tape measure protein)

Jacko_51

Jacko_52
(esterase)

Jacko_53

Jacko_54
(minor tail protein)

Jacko_55

Jacko_56

Jacko_57

Jacko_58
(endolysin)

Jacko_59

Jacko_60

Jacko_61
(RuvC-like resolvase)

Jacko_62

Jacko_63

Jacko_64

Jacko_65

Jacko_66
(DNA primase/polymerase)

Jacko_67
(HNH endonuclease)

Jacko_68

Jacko_69

Jacko_70

Jacko_71
(exonuclease)

Jacko_72

Jacko_73
(exonuclease)

Jacko_74

Jacko_75

Jacko_76

Jacko_77
(RNA ligase)

Jacko_78
(polynucleotide kinase)

Jacko_79
(DNA helicase)

Jacko_80

Jacko_81

Jacko_82

Jacko_83

Jacko_84

Jacko_85

Jacko_86

Jacko_87

Jacko_88

Jacko_89

Jacko_90

Jacko_91

Jacko_92

Jacko_93

Jacko_94

Jacko_95

Jacko_96
(helix-turn-helix DNA binding protein)

Jacko_97

Jacko_98

Jacko_99

Jacko_100

Jacko_101

Jacko_102

Jacko_103

Jacko_104

Jacko_105

Jacko_106

Jacko_107

Jacko_108

Jacko_109

Jacko_110

Jacko_111

Jacko_112

Jacko_113

Jacko_114

Jacko_115

Jacko_116

Jacko_117

Submitted Minimal DNA Master FileDownload
Publication Info
Uploaded to GenBank?Yes
GenBank AccessionMH399779
Refseq NumberNone yet
Archiving Info
Archiving status Archived
SEA Lysate Titer 2.035 x 10^8
Pitt Freezer Box# 69
Pitt Freezer Box Grid# G4
Available Files
Plaque PictureDownload
EM PictureDownload
GenBank File for PhameratorDownload