| Detailed Information for Phage Kubed | |
| Discovery Information | |
| Isolation Host | Propionibacterium acnes ATCC 6919 |
| Former names | TheChosen1 |
| Found By | Susy Mendez |
| Year Found | 2012 |
| Location Found | Los Angeles, CA USA |
| Finding Institution | University of California, Los Angeles |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | No |
| Isolation Temperature | 37°C |
| GPS Coordinates | 34.065 N, 118.4425 W Map |
| Discovery Notes | Phage isolation and genome annotation performed by Samantha Capati, Susy Mendez, and Jamie Pumford (UCLA). DNA preparation performed by Krisanavane Reddi (UCLA). Assembly performed by William Villella (UCLA) and confirmed by Charles Bowman (University of Pittsburgh). Quality control of annotation results performed by Jordan Moberg Parker and Dallas Mould (UCLA). |
| Sequencing Information | |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | May 26, 2015 |
| Sequencing Facility | The UCLA Genotyping and Sequencing Core |
| Shotgun Sequencing Method | 454 |
| Approximate Shotgun Coverage | 71 |
| Genome length (bp) | 29461 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 11 bases |
| Overhang Sequence | TCGTACGGCTT |
| GC Content | 54.5% |
| Fasta file available? | Yes: Download fasta file |
| Characterization | |
| Cluster | BU |
| Subcluster | -- |
| Cluster Life Cycle | Lytic |
| Other Cluster Members |
Click to ViewAquarius ATCC29399BC ATCC29399BT Attacne BruceLethal Cota DrParker Enochoraptor Enoki Keiki Kubed Lauchelly Leviosa LilBandit MEAK Moyashi MrAK Ouroboros P1.1 P100.1 P100A P100D P101A P104A P104B P105 P106A P106C P106I P106L P106M P107A P107C P108C P14.4 P9.1 PA6 PAD20 PAS50 Pirate Procrass1 QueenBey Rileysaurus SKKY Solid Stormborn Supernova Wizzo |
| Annotating Institution | Unknown or unassigned |
| Annotation Status | In GenBank |
| Plaque Notes | clear plaques; average diameter of 3 mm |
| Morphotype | Siphoviridae |
| Number of Genes | 44 |
| Number of tRNAs | 0 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
Click to ViewKubed_1 Kubed_2 Kubed_3 Kubed_4 Kubed_5 Kubed_6 Kubed_7 Kubed_8 Kubed_9 Kubed_10 Kubed_11 Kubed_12 Kubed_13 Kubed_14 Kubed_15 Kubed_16 Kubed_17 Kubed_18 Kubed_19 Kubed_20 Kubed_21 Kubed_22 Kubed_23 Kubed_24 Kubed_25 Kubed_26 Kubed_27 Kubed_28 Kubed_29 Kubed_30 Kubed_31 Kubed_32 Kubed_33 Kubed_34 Kubed_35 Kubed_36 Kubed_37 Kubed_38 Kubed_39 Kubed_40 Kubed_41 Kubed_42 Kubed_43 Kubed_44 |
| Publication Info | |
| Uploaded to GenBank? | Yes |
| GenBank Accession | KR337645 |
| Refseq Number | None yet |
| Archiving Info | |
| Archiving status | Archived |
| SEA Designator | 2011UCLAbruinVP |
| SEA Lysate Titer | 3.2x10^6 pfu/ml |
| Date of SEA Lysate Titering | May 22, 2012 |
| Pitt Freezer Box# | 28 |
| Pitt Freezer Box Grid# | D4 |
| Available Files | |
| Plaque Picture | Download |
| EM Picture | Download |
| Final DNAMaster File | Download |