| Detailed Information for Phage Leviosa | |
| Discovery Information | |
| Isolation Host | Propionibacterium acnes ATCC 6919 |
| Found By | Teams YCPWU and Plaquille O'Neil |
| Year Found | 2015 |
| Location Found | Los Angeles, CA USA |
| Finding Institution | University of California, Los Angeles |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | No |
| Isolation Temperature | 37°C |
| GPS Coordinates | 34.068900 N, 118.445200 W Map |
| Discovery Notes | Microcomedones (blackheads) were extracted from the nose of Andy Vu using a Biore Pore Strip on 04/06/2015. The skin was unwashed and slightly oily, and no moisturizer had been applied. Sample was enriched in RCM media with P. acnes strain ATCC 6919 under anaerobic conditions at 37oC. Phage Leviosa was isolated,characterized, and annotated by Team Plaquille O’Neal and Team You Can’t Phage with Us (YCPWU). Plaquille O'Neal: Connie Giang, Adilene Barajas, Andy Vu, Samantha Kaufhold YCPWU: Aparna Vancheswaran, Lariska Theron, Naeha Lakshmanan, Suruchi Salgar TA: Rocky Ng Instructor: Jordan Moberg Parker Of Note: Leviosa was able to lyse phage resistant P. acnes strain B101.9 (ribotype 2), which is known to contain CRISPR loci, and was not able to be lysed by other class phages, Cota and LilBandit. |
| Naming Notes | Named after the spell in Harry Potter. |
| Sequencing Information | |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Sep 11, 2015 |
| Sequencing Facility | NC State Genomic Sciences Laboratory |
| Shotgun Sequencing Method | Illumina |
| Approximate Shotgun Coverage | 6276 |
| Genome length (bp) | 29451 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 11 bases |
| Overhang Sequence | TCGTACGGCTT |
| GC Content | 53.7% |
| Fasta file available? | Yes: Download fasta file |
| Characterization | |
| Cluster | BU |
| Subcluster | -- |
| Cluster Life Cycle | Lytic |
| Lysogeny Notes | Leviosa is pseudolysogenic. The genome does not contain any known lysogeny genes, but lysogens that shed phage are able to be cultivated. |
| Other Cluster Members |
Click to ViewAquarius ATCC29399BC ATCC29399BT Attacne BruceLethal Cota DrParker Enochoraptor Enoki Keiki Kubed Lauchelly Leviosa LilBandit MEAK Moyashi MrAK Ouroboros P1.1 P100.1 P100A P100D P101A P104A P104B P105 P106A P106C P106I P106L P106M P107A P107C P108C P14.4 P9.1 PA6 PAD20 PAS50 Pirate Procrass1 QueenBey Rileysaurus SKKY Solid Stormborn Supernova Wizzo |
| Annotating Institution | Unknown or unassigned |
| Annotation Status | In GenBank |
| Plaque Notes | Bullseye morphology Average diameter: 8.5 mm Size range:710 mm |
| Morphotype | Siphoviridae |
| Number of Genes | 44 |
| Number of tRNAs | 0 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
Click to ViewLeviosa_1 Leviosa_2 Leviosa_3 Leviosa_4 Leviosa_5 Leviosa_6 Leviosa_7 Leviosa_8 Leviosa_9 Leviosa_10 Leviosa_11 Leviosa_12 Leviosa_13 Leviosa_14 Leviosa_15 Leviosa_16 Leviosa_17 Leviosa_18 Leviosa_19 Leviosa_20 Leviosa_21 Leviosa_22 Leviosa_23 Leviosa_24 Leviosa_25 Leviosa_26 Leviosa_27 Leviosa_28 Leviosa_29 Leviosa_30 Leviosa_31 Leviosa_32 Leviosa_33 Leviosa_34 Leviosa_35 Leviosa_36 Leviosa_37 Leviosa_38 Leviosa_39 Leviosa_40 Leviosa_41 Leviosa_42 Leviosa_43 Leviosa_44 |
| Submitted Annotation in DNA Master Format | Download |
| Submitted Minimal DNA Master File | Download |
| Author List | Download |
| Annotation Cover Sheet | Download |
| Publication Info | |
| Uploaded to GenBank? | Yes |
| GenBank Accession | MF919515 |
| Refseq Number | None yet |
| Archiving Info | |
| Archiving status | Archived |
| Pitt Freezer Box# | 23 |
| Pitt Freezer Box Grid# | E5 |
| Available Files | |
| Plaque Picture | Download |
| EM Picture | Download |
| GenBank File for Phamerator | Download |