| Detailed Information for Phage LilBandit | |
| Discovery Information | |
| Isolation Host | Propionibacterium acnes ATCC 6919 |
| Found By | Teams Lytic Squad and Unphaged |
| Year Found | 2015 |
| Location Found | Los Angeles, CA USA |
| Finding Institution | University of California, Los Angeles |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Unknown |
| Isolation Temperature | 37°C |
| GPS Coordinates | 34.068900 N, 118.445200 W Map |
| Discovery Notes | Microcomedones (blackheads) were extracted from the nose of Nancy Quintanilla using a Biore Pore Strip on April 3rd, 2015. The skin was unwashed and oily. Sample was enriched in RCM media with P. acnes strain ATCC 6919 under anaerobic conditions at 37C. Phage LilBandit was isolated, characterized, and annotated by Team The Lytic Squad (Kimberly Virgen, Benni Vargas, Nancy Quintanilla, Noor Jabaieh) and Team Unphaged (Asit Dave, Ewha Hong, Nick Rice, David Shabsovich). TA: Rocky Ng, Instructor: Jordan Moberg Parker, Laboratory Support: Krisanavane Reddi. |
| Naming Notes | Very small and hard to catch! |
| Sequencing Information | |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Sep 11, 2015 |
| Sequencing Facility | NC State Genomic Sciences Laboratory |
| Shotgun Sequencing Method | Illumina |
| Approximate Shotgun Coverage | 5622 |
| Genome length (bp) | 29041 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 11 bases |
| Overhang Sequence | TCGTACGGCTT |
| GC Content | 54.0% |
| Fasta file available? | Yes: Download fasta file |
| Characterization | |
| Cluster | BU |
| Subcluster | -- |
| Cluster Life Cycle | Lytic |
| Lysogeny Notes | Plaques are generally clear. |
| Other Cluster Members |
Click to ViewAquarius ATCC29399BC ATCC29399BT Attacne BruceLethal Cota DrParker Enochoraptor Enoki Keiki Kubed Lauchelly Leviosa LilBandit MEAK Moyashi MrAK Ouroboros P1.1 P100.1 P100A P100D P101A P104A P104B P105 P106A P106C P106I P106L P106M P107A P107C P108C P14.4 P9.1 PA6 PAD20 PAS50 Pirate Procrass1 QueenBey Rileysaurus SKKY Solid Stormborn Supernova Wizzo |
| Annotating Institution | Unknown or unassigned |
| Annotation Status | In GenBank |
| Plaque Notes | LilBandit generally produces small clear plaques, although some plaques can be much larger. LilBandit was repeatedly purified through plaque assays, and it is believed that the mixed morphologies is a characteristic of the phage. |
| Morphotype | Siphoviridae |
| Number of Genes | 45 |
| Number of tRNAs | 0 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
Click to ViewLilBandit_1 LilBandit_2 LilBandit_3 LilBandit_4 LilBandit_5 LilBandit_6 LilBandit_7 LilBandit_8 LilBandit_9 LilBandit_10 LilBandit_11 LilBandit_12 LilBandit_13 LilBandit_14 LilBandit_15 LilBandit_16 LilBandit_17 LilBandit_18 LilBandit_19 LilBandit_20 LilBandit_21 LilBandit_22 LilBandit_23 LilBandit_24 LilBandit_25 LilBandit_26 LilBandit_27 LilBandit_28 LilBandit_29 LilBandit_30 LilBandit_31 LilBandit_32 LilBandit_33 LilBandit_34 LilBandit_35 LilBandit_36 LilBandit_37 LilBandit_38 LilBandit_39 LilBandit_40 LilBandit_41 LilBandit_42 LilBandit_43 LilBandit_44 LilBandit_45 |
| Submitted Minimal DNA Master File | Download |
| Publication Info | |
| Uploaded to GenBank? | Yes |
| GenBank Accession | MF919516 |
| Refseq Number | None yet |
| Archiving Info | |
| Archiving status | Archived |
| Pitt Freezer Box# | 28 |
| Pitt Freezer Box Grid# | D11 |
| Available Files | |
| Plaque Picture | Download |
| EM Picture | Download |
| GenBank File for Phamerator | Download |