| Discovery Information |
| Isolation Host | Streptomyces mirabilis NRRL B-2400 |
| Found By | Peyton Heiss & Amma Frimpong |
| Year Found | 2025 |
| Location Found | Severn, MD United States of America |
| Finding Institution | University of Maryland, Baltimore County |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | No |
| Isolation Temperature | 30°C |
| GPS Coordinates | 39.126164 N, 76.679023 W Map |
| Discovery Notes | Discovered in a soil sample that was collected from a moist, sandy, area. The soil had recently rain and was positioned in a flat grassy roadside area. The outdoor temperature was 17C at the time of collection. |
| Naming Notes | The name was inspired by the place it was discovered. LilSaint was found in the front yard of a pastor's home. |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Jan 5, 2026 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina |
| Sequencer Used | Illumina NextSeq 1000 |
| Read Type | Single-end reads |
| Read Length | 100 bp |
| Approximate Shotgun Coverage | 336 |
| Genome length (bp) | 132797 |
| Character of genome ends | Direct Terminal Repeat |
| Overhang Length | 10947 bases |
| GC Content | 49.7% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | BE |
| Subcluster | BE1 |
| Cluster Life Cycle | Lytic |
| Lysogeny Notes | A lawn of S. mirabilis was spotted with LilSaint and incubated for 7 days. After 7 days, plaques did not show signs of lysogenic growth, indicating that LilSaint may be a virulent phage. |
| Other Cluster Members |
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| Annotating Institution | University of Maryland, Baltimore County |
| Annotation Status | Being Annotated (Expected completion by 5/1/2026) |
| Plaque Notes | Clear, round, rough edges. Plaques are ~2mm in diameter |
| Morphotype | Siphoviridae |
| Number of Genes | 248 |
| Number of tRNAs | 40 |
| Number of tmRNAs | 1 |
| Has been Phamerated? | Yes |
| Gene List |
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| Publication Info |
| Uploaded to GenBank? | No |
| GenBank Accession | None yet |
| Refseq Number | None yet |
| Archiving Info |
| Archiving status |
Not in Pitt Archives |
| Available Files |
| Restriction Digest Picture | Download |
| EM Picture | Download |
