| Detailed Information for Phage LonelyBoi | |
| Discovery Information | |
| Isolation Host | Gordonia terrae 3612 |
| Found By | J. Axelson, E. Lider, S. Lord, & S. Wolf |
| Year Found | 2020 |
| Location Found | Squirrel Hill, Pittsburgh, PA United States |
| Finding Institution | University of Pittsburgh |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | No |
| Isolation Temperature | 30°C |
| GPS Coordinates | 40.426667 N, 79.905833 W Map |
| Discovery Notes | The soil sample was found in Frick Park near Dencreek and was given to us by a peer. Upon using this soil sample, we found it to be dry, had no evidence of fertilizer, was fine in texture, and did not have any large chunks of soil or wood. N.B.: The full names of all those who discovered the phage are as follows: Jessica Axelson, Ebru Lider, Samuel Lord, & Sarah Wolf |
| Naming Notes | This phage was named after the fact that it only appeared after extra days of incubation and only developed as one tiny, "lonely" clearing on the plate that we almost missed due to its size and "loneliness." |
| Sequencing Information | |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Jan 21, 2021 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina |
| Sequencer Used | Illumina MiSeq |
| Read Type | Single-end reads |
| Read Length | 150 bp |
| Approximate Shotgun Coverage | 1112 |
| Genome length (bp) | 53604 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 10 bases |
| Overhang Sequence | CGTATGGCAT |
| GC Content | 66.4% |
| Fasta file available? | Yes: Download fasta file |
| Characterization | |
| Cluster | CY |
| Subcluster | -- |
| Cluster Life Cycle | Temperate |
| Other Cluster Members | |
| Annotating Institution | University of Pittsburgh |
| Annotation Status | In GenBank |
| Plaque Notes | Produces small(~ 1 mm in diameter), circular plaques that seem to be turbid around the edges |
| Number of Genes | 78 |
| Number of tRNAs | 0 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
Click to ViewLonelyBoi_1 LonelyBoi_2 LonelyBoi_3 LonelyBoi_4 LonelyBoi_5 LonelyBoi_6 LonelyBoi_7 LonelyBoi_8 LonelyBoi_9 LonelyBoi_10 LonelyBoi_11 LonelyBoi_12 LonelyBoi_13 LonelyBoi_14 LonelyBoi_15 LonelyBoi_16 LonelyBoi_17 LonelyBoi_18 LonelyBoi_19 LonelyBoi_20 LonelyBoi_21 LonelyBoi_22 LonelyBoi_23 LonelyBoi_24 LonelyBoi_25 LonelyBoi_26 LonelyBoi_27 LonelyBoi_28 LonelyBoi_29 LonelyBoi_30 LonelyBoi_31 LonelyBoi_32 LonelyBoi_33 LonelyBoi_34 LonelyBoi_35 LonelyBoi_36 LonelyBoi_37 LonelyBoi_38 LonelyBoi_39 LonelyBoi_40 LonelyBoi_41 LonelyBoi_42 LonelyBoi_43 LonelyBoi_44 LonelyBoi_45 LonelyBoi_46 LonelyBoi_47 LonelyBoi_48 LonelyBoi_49 LonelyBoi_50 LonelyBoi_51 LonelyBoi_52 LonelyBoi_53 LonelyBoi_54 LonelyBoi_55 LonelyBoi_56 LonelyBoi_57 LonelyBoi_58 LonelyBoi_59 LonelyBoi_60 LonelyBoi_61 LonelyBoi_62 LonelyBoi_63 LonelyBoi_64 LonelyBoi_65 LonelyBoi_66 LonelyBoi_67 LonelyBoi_68 LonelyBoi_69 LonelyBoi_70 LonelyBoi_71 LonelyBoi_72 LonelyBoi_73 LonelyBoi_74 LonelyBoi_75 LonelyBoi_76 LonelyBoi_77 LonelyBoi_78 |
| Submitted Minimal DNA Master File | Download |
| Publication Info | |
| Uploaded to GenBank? | Yes |
| GenBank Accession | MZ622165 |
| Refseq Number | None yet |
| Archiving Info | |
| Archiving status | Archived |
| Pitt Freezer Box# | 125 |
| Pitt Freezer Box Grid# | F12 |
| Available Files | |
| GenBank File for Phamerator | Download |