| Detailed Information for Phage LonelyBoi |
| Discovery Information |
| Isolation Host | Gordonia terrae 3612 |
| Found By | J. Axelson, E. Lider, S. Lord, & S. Wolf |
| Year Found | 2020 |
| Location Found | Squirrel Hill, Pittsburgh, PA United States |
| Finding Institution | University of Pittsburgh |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | No |
| Isolation Temperature | 30°C |
| GPS Coordinates | 40.426667 N, 79.905833 W Map |
| Discovery Notes | The soil sample was found in Frick Park near Dencreek and was given to us by a peer. Upon using this soil sample, we found it to be dry, had no evidence of fertilizer, was fine in texture, and did not have any large chunks of soil or wood.
N.B.: The full names of all those who discovered the phage are as follows:
Jessica Axelson, Ebru Lider, Samuel Lord, & Sarah Wolf |
| Naming Notes | This phage was named after the fact that it only appeared after extra days of incubation and only developed as one tiny, "lonely" clearing on the plate that we almost missed due to its size and "loneliness." |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Jan 21, 2021 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina |
| Approximate Shotgun Coverage | 1112 |
| Genome length (bp) | 53604 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 10 bases |
| Overhang Sequence | CGTATGGCAT |
| GC Content | 66.4% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | CY |
| Subcluster | -- |
| Cluster Life Cycle | Temperate |
| Other Cluster Members |
|
| Annotating Institution | University of Pittsburgh |
| Annotation Status | In GenBank |
| Plaque Notes | Produces small(~ 1 mm in diameter), circular plaques that seem to be turbid around the edges |
| Number of Genes | 78 |
| Number of tRNAs | 0 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Minimal DNA Master File | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | MZ622165 |
| Refseq Number | None yet |
| Archiving Info |
| Archiving status |
Archived |
| Pitt Freezer Box# |
125 |
| Pitt Freezer Box Grid# |
F12 |
| Available Files |
| GenBank File for Phamerator | Download |