The Actinobacteriophage Database

Streptomyces phage LukeCage

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(S. griseus NBRC13350)

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(S. scabiei 82-22)

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Detailed Information for Phage LukeCage
Discovery Information
Isolation Host	Streptomyces griseus ATCC 10137
Found By	Ben Gillick
Year Found	2017
Location Found	Dallas, TX USA
Finding Institution	University of North Texas
Program	Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?	No
Isolation Temperature	28°C
GPS Coordinates	32.749208 N, 96.95602 W Map
Discovery Notes	Soil was taken on September 4th, 2017 at approximately 10:30pm. Despite having rained that morning, the soil was loose and dry, containing a small amount of loose debris(small pieces of leaves, small twigs)
Naming Notes	Luke Cage is a cool marvel character. Also cage rhymes with phage so that's fun.
Sequencing Information
Sequencing Complete?	Yes
Date Sequencing Completed	Dec 14, 2017
Sequencing Facility	Pittsburgh Bacteriophage Institute
Shotgun Sequencing Method	Illumina
Genome length (bp)	133195
Character of genome ends	Unknown
Direct Terminal Repeat Length	12291 bp
GC Content	49.0%
Fasta file available?	Yes: Download fasta file
Characterization
Cluster	BE
Subcluster	BE2
Cluster Life Cycle	Lytic
Other Cluster Members	Click to View Anedea (BE1) Angela (BE1) Bartholomune (BE1) BlueOtter (BE1) Bmoc (BE1) Braelyn (BE1) Cross (BE1) Cursive (BE1) Daubenski (BE1) EGole (BE1) Evy (BE1) HangryHippo (BE1) Jay2Jay (BE1) Larnav (BE1) Leo04 (BE1) Liandry (BE1) LilMartin (BE1) Lululemon (BE1) Mildred21 (BE1) MulchMansion (BE1) Navo (BE1) NootNoot (BE1) PacManQ (BE1) Paradiddles (BE1) Peebs (BE1) Pepperwood (BE1) Persimmon (BE1) PinkiePie (BE1) Riptide (BE1) Samisti12 (BE1) Scheme (BE1) Squillium (BE1) Sushi23 (BE1) Targaryen (BE1) Teutsch (BE1) Tribute (BE1) Warpy (BE1) Watermoore (BE1) WhereRU (BE1) Amabiko (BE2) Battuta (BE2) Birchlyn (BE2) BoomerJR (BE2) Bordeaux (BE2) CeilingFan (BE2) Elmer (BE2) Enygma (BE2) Genie2 (BE2) Gibbi (BE2) IchabodCrane (BE2) JimJam (BE2) Jollison (BE2) Karimac (BE2) KentuckyRacer (BE2) LukeCage (BE2) MindFlayer (BE2) Mugiwara (BE2) PumpkinSpice (BE2) Quaran19 (BE2) SaltySpitoon (BE2) Sollertia (BE2) Spelly (BE2) Spilled (BE2) Stanimal (BE2) Starbow (BE2) StarPlatinum (BE2) Tomas (BE2) TomSawyer (BE2) Wipeout (BE2) Wofford (BE2) Yaboi (BE2)
Annotating Institution	University of North Texas
Annotation Status	In GenBank
Plaque Notes	Nearly all plaques were circular and symmetrical, averaging between .9 and 1.1 mm in diameter. They are all very clear with very little turbidity. Some plaques around the edges are in close proximity and come together which gives the illusion of slightly turbid edges. In plaques around edges circular shape is not as uniform and plaques look slightly more free-form than a typical circle due to the higher density in this area.
Morphotype	Siphoviridae
Number of Genes	248
Number of tRNAs	41
Number of tmRNAs	1
Has been Phamerated?	Yes
Gene List	Click to View LukeCage_1 LukeCage_2 LukeCage_3 LukeCage_4 LukeCage_5 LukeCage_6 (Lsr2-like DNA bridging protein) LukeCage_7 LukeCage_8 LukeCage_9 LukeCage_10 LukeCage_11 LukeCage_12 (ParB-like nuclease domain protein) LukeCage_13 LukeCage_14 LukeCage_15 LukeCage_16 LukeCage_17 LukeCage_18 LukeCage_19 LukeCage_20 LukeCage_21 LukeCage_22 LukeCage_23 LukeCage_24 LukeCage_25 LukeCage_26 LukeCage_27 (HNH endonuclease) LukeCage_28 LukeCage_29 (ThyX-like thymidylate synthase) LukeCage_30 LukeCage_31 LukeCage_32 LukeCage_33 LukeCage_34 LukeCage_35 LukeCage_36 LukeCage_37 LukeCage_38 — tRNA (tRNA-Thr(cgt)) LukeCage_39 (HNH endonuclease) LukeCage_40 (endolysin) LukeCage_41 LukeCage_42 LukeCage_43 (glycosyltransferase) LukeCage_44 (ribonucleotide reductase) LukeCage_45 (LysM-like peptidoglycan-binding protein) LukeCage_46 LukeCage_47 LukeCage_48 (portal protein) LukeCage_49 LukeCage_50 (capsid maturation protease) LukeCage_51 (major capsid protein) LukeCage_52 LukeCage_53 LukeCage_54 LukeCage_55 LukeCage_56 LukeCage_57 (major tail protein) LukeCage_58 (tail assembly chaperone) LukeCage_59 LukeCage_60 (tape measure protein) LukeCage_61 LukeCage_62 (minor tail protein) LukeCage_63 LukeCage_64 (minor tail protein) LukeCage_65 (minor tail protein) LukeCage_66 LukeCage_67 LukeCage_68 LukeCage_69 (HNH endonuclease) LukeCage_70 (metallophosphoesterase) LukeCage_71 LukeCage_72 LukeCage_73 LukeCage_74 LukeCage_75 LukeCage_76 LukeCage_77 (DNA helicase) LukeCage_78 LukeCage_79 (DNA primase) LukeCage_80 (DNA binding protein) LukeCage_81 (DnaE-like DNA polymerase III alpha) LukeCage_82 LukeCage_83 (MazG-like nucleotide pyrophosphohydrolase) LukeCage_84 (RecA-like DNA recombinase) LukeCage_85 LukeCage_86 (holliday junction resolvase) LukeCage_87 LukeCage_88 LukeCage_89 LukeCage_90 (Cas4 family exonuclease) LukeCage_91 LukeCage_92 (RuvC-like resolvase) LukeCage_93 (DprA-like ssDNA binding protein) LukeCage_94 LukeCage_95 (methyltransferase) LukeCage_96 (thymidylate kinase) LukeCage_97 LukeCage_98 LukeCage_99 (terminase) LukeCage_100 (HNH endonuclease) LukeCage_101 LukeCage_102 — tRNA (tRNA-Gly(tcc)) LukeCage_103 — tRNA (tRNA-Gln(ctg)) LukeCage_104 — tRNA (tRNA-Trp(cca)) LukeCage_105 LukeCage_106 LukeCage_107 — tRNA (tRNA-Pro(tgg)) LukeCage_108 — tRNA (tRNA-Pro(tgg)) LukeCage_109 (nucleotidyltransferase) LukeCage_110 (FtsK-like DNA translocase) LukeCage_111 LukeCage_112 — tRNA (tRNA-Ile(tat)) LukeCage_113 LukeCage_114 LukeCage_115 LukeCage_116 — tRNA (tRNA-Ser(gga)) LukeCage_117 LukeCage_118 LukeCage_119 LukeCage_120 LukeCage_121 LukeCage_122 LukeCage_123 — tRNA (tRNA-Asp(gtc)) LukeCage_124 LukeCage_125 LukeCage_126 LukeCage_127 — tRNA (tRNA-Asp(gtc)) LukeCage_128 LukeCage_129 (Ro-like RNA binding protein) LukeCage_130 LukeCage_131 LukeCage_132 LukeCage_133 LukeCage_134 (DNA binding protein) LukeCage_135 LukeCage_136 LukeCage_137 LukeCage_138 LukeCage_139 LukeCage_140 — tRNA (tRNA-Glu(ctc)) LukeCage_141 LukeCage_142 LukeCage_143 — tRNA (tRNA-Glu(ttc)) LukeCage_144 — tRNA (tRNA-Val(tac)) LukeCage_145 — tRNA (tRNA-Leu(tag)) LukeCage_146 — tRNA (tRNA-Leu(tag)) LukeCage_147 — tRNA (tRNA-Phe(gaa)) LukeCage_148 — tRNA (tRNA-Gly(gcc)) LukeCage_149 — tRNA (tRNA-Arg(tct)) LukeCage_150 LukeCage_151 LukeCage_152 LukeCage_153 — tRNA (tRNA-Ala(tgc)) LukeCage_154 — tRNA (tRNA-Lys(ctt)) LukeCage_155 (peptidase) LukeCage_156 LukeCage_157 LukeCage_158 — tRNA (tRNA-Asn(gtt)) LukeCage_159 LukeCage_160 LukeCage_161 — tRNA (tRNA-Asn(gtt)) LukeCage_162 (band-7-like membrane protein) LukeCage_163 LukeCage_164 LukeCage_165 — tRNA (tRNA-Arg(cct)) LukeCage_166 — tRNA (tRNA-Ala(ggc)) LukeCage_167 — tRNA (tRNA-Lys(ctt)) LukeCage_168 — tRNA (tRNA-Lys(ctt)) LukeCage_169 LukeCage_170 — tRNA (tRNA-Lys(ttt)) LukeCage_171 LukeCage_172 — tRNA (tRNA-Arg(tcg)) LukeCage_173 LukeCage_174 — tRNA (tRNA-Met(cat)) LukeCage_175 LukeCage_176 LukeCage_177 — tRNA (tRNA-Tyr(gta)) LukeCage_178 — tRNA (tRNA-Tyr(gta)) LukeCage_179 — tRNA (tRNA-His(gtg)) LukeCage_180 LukeCage_181 LukeCage_182 LukeCage_183 — tRNA (tRNA-Cys(gca)) LukeCage_184 — tRNA (tRNA-Thr(tgt)) LukeCage_185 — tRNA (tRNA-Thr(ggt)) LukeCage_186 LukeCage_187 — tRNA (tRNA-Arg(acg)) LukeCage_188 LukeCage_189 (DnaQ-like DNA polymerase III subunit) LukeCage_190 LukeCage_191 LukeCage_192 — tmRNA (tmRNA) LukeCage_193 LukeCage_194 LukeCage_195 LukeCage_196 LukeCage_197 LukeCage_198 LukeCage_199 — tRNA (tRNA-Ala(ggc)) LukeCage_200 LukeCage_201 LukeCage_202 LukeCage_203 LukeCage_204 LukeCage_205 LukeCage_206 LukeCage_207 (RNA ligase) LukeCage_208 LukeCage_209 LukeCage_210 (polynucleotide kinase) LukeCage_211 LukeCage_212 LukeCage_213 LukeCage_214 LukeCage_215 LukeCage_216 — tRNA (tRNA-Leu(ctc)) LukeCage_217 LukeCage_218 LukeCage_219 LukeCage_220 — tRNA (tRNA-Val(gtc)) LukeCage_221 (SprT-like protease) LukeCage_222 LukeCage_223 LukeCage_224 — tRNA (tRNA-Leu(ttg)) LukeCage_225 LukeCage_226 LukeCage_227 LukeCage_228 LukeCage_229 LukeCage_230 LukeCage_231 LukeCage_232 LukeCage_233 LukeCage_234 (deoxycytidylate deaminase) LukeCage_235 (phosphoesterase) LukeCage_236 LukeCage_237 LukeCage_238 (WhiB family transcription factor) LukeCage_239 LukeCage_240 LukeCage_241 LukeCage_242 LukeCage_243 LukeCage_244 LukeCage_245 LukeCage_246 LukeCage_247 LukeCage_248 LukeCage_249 — tRNA (tRNA-Gln(caa)) LukeCage_250 LukeCage_251 LukeCage_252 LukeCage_253 LukeCage_254 LukeCage_255 (thioredoxin) LukeCage_256 LukeCage_257 LukeCage_258 LukeCage_259 LukeCage_260 LukeCage_261 LukeCage_262 LukeCage_263 LukeCage_264 LukeCage_265 LukeCage_266 LukeCage_267 LukeCage_268 LukeCage_269 (Lsr2-like DNA bridging protein) LukeCage_270 LukeCage_271 LukeCage_272 LukeCage_273 LukeCage_274 LukeCage_275 (ParB-like nuclease domain protein) LukeCage_276 LukeCage_277 LukeCage_278 LukeCage_279 LukeCage_280 LukeCage_281 LukeCage_282 LukeCage_283 LukeCage_284 LukeCage_285 LukeCage_286 LukeCage_287 LukeCage_288 LukeCage_289 LukeCage_290 (HNH endonuclease)
Submitted Minimal DNA Master File	Download
Publication Info
Uploaded to GenBank?	Yes
GenBank Accession	MH590597
Refseq Number	None yet
Archiving Info
Archiving status	Archived
Pitt Freezer Box#	70
Pitt Freezer Box Grid#	C5
Available Files
Plaque Picture	Download
Restriction Digest Picture	Download
EM Picture	Download
GenBank File for Phamerator	Download

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