Streptomyces phage LukeCage
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Detailed Information for Phage LukeCage
Discovery Information
Isolation HostStreptomyces griseus ATCC 10137
Found ByBen Gillick
Year Found2017
Location FoundDallas, TX USA
Finding InstitutionUniversity of North Texas
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?No
Isolation Temperature28°C
GPS Coordinates32.749208 N, 96.95602 W Map
Discovery NotesSoil was taken on September 4th, 2017 at approximately 10:30pm.
Despite having rained that morning, the soil was loose and dry, containing a small amount of loose debris(small pieces of leaves, small twigs)
Naming NotesLuke Cage is a cool marvel character. Also cage rhymes with phage so that's fun.
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedDec 14, 2017
Sequencing FacilityPittsburgh Bacteriophage Institute
Shotgun Sequencing MethodIllumina Sequencing
Genome length (bp)133195
Character of genome endsUnknown
Direct Terminal Repeat Length12291 bp
GC Content49.0%
Fasta file available?Yes: Download fasta file
Characterization
ClusterBE
SubclusterBE2
Cluster Life CycleLytic
Other Cluster Members
Annotating InstitutionUniversity of North Texas
Annotation StatusIn GenBank
Plaque NotesNearly all plaques were circular and symmetrical, averaging between .9 and 1.1 mm in diameter. They are all very clear with very little turbidity. Some plaques around the edges are in close proximity and come together which gives the illusion of slightly turbid edges. In plaques around edges circular shape is not as uniform and plaques look slightly more free-form than a typical circle due to the higher density in this area.
MorphotypeSiphoviridae
Number of Genes248
Number of tRNAs41
Number of tmRNAs1
Has been Phamerated?Yes
Gene List

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LukeCage_1

LukeCage_2

LukeCage_3

LukeCage_4

LukeCage_5

LukeCage_6
(Lsr2-like DNA bridging protein)

LukeCage_7

LukeCage_8

LukeCage_9

LukeCage_10

LukeCage_11

LukeCage_12
(ParB-like nuclease domain protein)

LukeCage_13

LukeCage_14

LukeCage_15

LukeCage_16

LukeCage_17

LukeCage_18

LukeCage_19

LukeCage_20

LukeCage_21

LukeCage_22

LukeCage_23

LukeCage_24

LukeCage_25

LukeCage_26

LukeCage_27
(HNH endonuclease)

LukeCage_28

LukeCage_29
(ThyX-like thymidylate synthase)

LukeCage_30

LukeCage_31

LukeCage_32

LukeCage_33

LukeCage_34

LukeCage_35

LukeCage_36

LukeCage_37

LukeCage_38 — tRNA
(tRNA-Thr(cgt))

LukeCage_39
(HNH endonuclease)

LukeCage_40
(endolysin)

LukeCage_41

LukeCage_42

LukeCage_43
(glycosyltransferase)

LukeCage_44
(ribonucleotide reductase)

LukeCage_45
(LysM-like peptidoglycan-binding protein)

LukeCage_46

LukeCage_47

LukeCage_48
(portal protein)

LukeCage_49

LukeCage_50
(capsid maturation protease)

LukeCage_51
(major capsid protein)

LukeCage_52

LukeCage_53

LukeCage_54

LukeCage_55

LukeCage_56

LukeCage_57
(major tail protein)

LukeCage_58
(tail assembly chaperone)

LukeCage_59

LukeCage_60
(tape measure protein)

LukeCage_61

LukeCage_62
(minor tail protein)

LukeCage_63

LukeCage_64
(minor tail protein)

LukeCage_65
(minor tail protein)

LukeCage_66

LukeCage_67

LukeCage_68

LukeCage_69
(HNH endonuclease)

LukeCage_70
(metallophosphoesterase)

LukeCage_71

LukeCage_72

LukeCage_73

LukeCage_74

LukeCage_75

LukeCage_76

LukeCage_77
(DNA helicase)

LukeCage_78

LukeCage_79
(DNA primase)

LukeCage_80
(DNA binding protein)

LukeCage_81
(DnaE-like DNA polymerase III alpha)

LukeCage_82

LukeCage_83
(MazG-like nucleotide pyrophosphohydrolase)

LukeCage_84
(RecA-like DNA recombinase)

LukeCage_85

LukeCage_86
(holliday junction resolvase)

LukeCage_87

LukeCage_88

LukeCage_89

LukeCage_90
(Cas4 family exonuclease)

LukeCage_91

LukeCage_92
(RuvC-like resolvase)

LukeCage_93
(DprA-like ssDNA binding protein)

LukeCage_94

LukeCage_95
(methyltransferase)

LukeCage_96
(thymidylate kinase)

LukeCage_97

LukeCage_98

LukeCage_99
(terminase)

LukeCage_100
(HNH endonuclease)

LukeCage_101

LukeCage_102 — tRNA
(tRNA-Gly(tcc))

LukeCage_103 — tRNA
(tRNA-Gln(ctg))

LukeCage_104 — tRNA
(tRNA-Trp(cca))

LukeCage_105

LukeCage_106

LukeCage_107 — tRNA
(tRNA-Pro(tgg))

LukeCage_108 — tRNA
(tRNA-Pro(tgg))

LukeCage_109
(nucleotidyltransferase)

LukeCage_110
(FtsK-like DNA translocase)

LukeCage_111

LukeCage_112 — tRNA
(tRNA-Ile(tat))

LukeCage_113

LukeCage_114

LukeCage_115

LukeCage_116 — tRNA
(tRNA-Ser(gga))

LukeCage_117

LukeCage_118

LukeCage_119

LukeCage_120

LukeCage_121

LukeCage_122

LukeCage_123 — tRNA
(tRNA-Asp(gtc))

LukeCage_124

LukeCage_125

LukeCage_126

LukeCage_127 — tRNA
(tRNA-Asp(gtc))

LukeCage_128

LukeCage_129
(Ro-like RNA binding protein)

LukeCage_130

LukeCage_131

LukeCage_132

LukeCage_133

LukeCage_134
(DNA binding protein)

LukeCage_135

LukeCage_136

LukeCage_137

LukeCage_138

LukeCage_139

LukeCage_140 — tRNA
(tRNA-Glu(ctc))

LukeCage_141

LukeCage_142

LukeCage_143 — tRNA
(tRNA-Glu(ttc))

LukeCage_144 — tRNA
(tRNA-Val(tac))

LukeCage_145 — tRNA
(tRNA-Leu(tag))

LukeCage_146 — tRNA
(tRNA-Leu(tag))

LukeCage_147 — tRNA
(tRNA-Phe(gaa))

LukeCage_148 — tRNA
(tRNA-Gly(gcc))

LukeCage_149 — tRNA
(tRNA-Arg(tct))

LukeCage_150

LukeCage_151

LukeCage_152

LukeCage_153 — tRNA
(tRNA-Ala(tgc))

LukeCage_154 — tRNA
(tRNA-Lys(ctt))

LukeCage_155
(peptidase)

LukeCage_156

LukeCage_157

LukeCage_158 — tRNA
(tRNA-Asn(gtt))

LukeCage_159

LukeCage_160

LukeCage_161 — tRNA
(tRNA-Asn(gtt))

LukeCage_162
(band-7-like membrane protein)

LukeCage_163

LukeCage_164

LukeCage_165 — tRNA
(tRNA-Arg(cct))

LukeCage_166 — tRNA
(tRNA-Ala(ggc))

LukeCage_167 — tRNA
(tRNA-Lys(ctt))

LukeCage_168 — tRNA
(tRNA-Lys(ctt))

LukeCage_169

LukeCage_170 — tRNA
(tRNA-Lys(ttt))

LukeCage_171

LukeCage_172 — tRNA
(tRNA-Arg(tcg))

LukeCage_173

LukeCage_174 — tRNA
(tRNA-Met(cat))

LukeCage_175

LukeCage_176

LukeCage_177 — tRNA
(tRNA-Tyr(gta))

LukeCage_178 — tRNA
(tRNA-Tyr(gta))

LukeCage_179 — tRNA
(tRNA-His(gtg))

LukeCage_180

LukeCage_181

LukeCage_182

LukeCage_183 — tRNA
(tRNA-Cys(gca))

LukeCage_184 — tRNA
(tRNA-Thr(tgt))

LukeCage_185 — tRNA
(tRNA-Thr(ggt))

LukeCage_186

LukeCage_187 — tRNA
(tRNA-Arg(acg))

LukeCage_188

LukeCage_189
(DnaQ-like DNA polymerase III subunit)

LukeCage_190

LukeCage_191

LukeCage_192 — tmRNA
(tmRNA)

LukeCage_193

LukeCage_194

LukeCage_195

LukeCage_196

LukeCage_197

LukeCage_198

LukeCage_199 — tRNA
(tRNA-Ala(ggc))

LukeCage_200

LukeCage_201

LukeCage_202

LukeCage_203

LukeCage_204

LukeCage_205

LukeCage_206

LukeCage_207
(RNA ligase)

LukeCage_208

LukeCage_209

LukeCage_210
(polynucleotide kinase)

LukeCage_211

LukeCage_212

LukeCage_213

LukeCage_214

LukeCage_215

LukeCage_216 — tRNA
(tRNA-Leu(ctc))

LukeCage_217

LukeCage_218

LukeCage_219

LukeCage_220 — tRNA
(tRNA-Val(gtc))

LukeCage_221
(SprT-like protease)

LukeCage_222

LukeCage_223

LukeCage_224 — tRNA
(tRNA-Leu(ttg))

LukeCage_225

LukeCage_226

LukeCage_227

LukeCage_228

LukeCage_229

LukeCage_230

LukeCage_231

LukeCage_232

LukeCage_233

LukeCage_234
(deoxycytidylate deaminase)

LukeCage_235
(phosphoesterase)

LukeCage_236

LukeCage_237

LukeCage_238
(WhiB family transcription factor)

LukeCage_239

LukeCage_240

LukeCage_241

LukeCage_242

LukeCage_243

LukeCage_244

LukeCage_245

LukeCage_246

LukeCage_247

LukeCage_248

LukeCage_249 — tRNA
(tRNA-Gln(caa))

LukeCage_250

LukeCage_251

LukeCage_252

LukeCage_253

LukeCage_254

LukeCage_255
(thioredoxin)

LukeCage_256

LukeCage_257

LukeCage_258

LukeCage_259

LukeCage_260

LukeCage_261

LukeCage_262

LukeCage_263

LukeCage_264

LukeCage_265

LukeCage_266

LukeCage_267

LukeCage_268

LukeCage_269
(Lsr2-like DNA bridging protein)

LukeCage_270

LukeCage_271

LukeCage_272

LukeCage_273

LukeCage_274

LukeCage_275
(ParB-like nuclease domain protein)

LukeCage_276

LukeCage_277

LukeCage_278

LukeCage_279

LukeCage_280

LukeCage_281

LukeCage_282

LukeCage_283

LukeCage_284

LukeCage_285

LukeCage_286

LukeCage_287

LukeCage_288

LukeCage_289

LukeCage_290
(HNH endonuclease)

Submitted Minimal DNA Master FileDownload
Publication Info
Uploaded to GenBank?Yes
GenBank AccessionMH590597
Refseq NumberNone yet
Archiving Info
Archiving status Archived
Pitt Freezer Box# 70
Pitt Freezer Box Grid# C5
Available Files
Plaque PictureDownload
Restriction Digest PictureDownload
EM PictureDownload
GenBank File for PhameratorDownload