| Detailed Information for Phage MEAK | |
| Discovery Information | |
| Isolation Host | Propionibacterium acnes ATCC 6919 |
| Found By | Baby Got Plaque |
| Year Found | 2016 |
| Location Found | Los Angeles, CA USA |
| Finding Institution | University of California, Los Angeles |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Unknown |
| Isolation Temperature | 37°C |
| GPS Coordinates | 34.068582 N, 118.441285 W Map |
| Discovery Notes | Phage isolation, characterization, and annotation were performed by Team Baby Got Plaque: Aida Akopyan, Emily Pang, Kei Shao, and Meera Solanki. The phage was isolated by direct plating of nasal microcomedones obtained by a Biore pore strip from the nose of Meera Solanki. TAs: Rocky Ng, Andrew Lund UA: Lariska Theron Instructors: Dr. Frances Mercer, Dr. Krisanavane Reddi and Dr. Jordan Moberg Parker. |
| Naming Notes | The students combined their first letter of their names to create MEAK (Meera, Emily, Aida, and Kei). |
| Sequencing Information | |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Aug 26, 2016 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina Sequencing |
| Approximate Shotgun Coverage | 5746 |
| Genome length (bp) | 29223 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 11 bases |
| Overhang Sequence | TCGTACGGCTT |
| GC Content | 54.0% |
| Fasta file available? | Yes: Download fasta file |
| Characterization | |
| Cluster | BU |
| Subcluster | -- |
| Cluster Life Cycle | Lytic |
| Lysogeny Notes | Plaque morphology and lack of lysogeny genes suggest this is a pseudolysogenic phage. |
| Other Cluster Members |
Click to ViewAquarius ATCC29399BC ATCC29399BT Attacne BruceLethal Cota DrParker Enochoraptor Enoki Keiki Kubed Lauchelly Leviosa LilBandit MEAK Moyashi MrAK Ouroboros P1.1 P100.1 P100A P100D P101A P104A P104B P105 P106A P106C P106I P106L P106M P107A P107C P108C P14.4 P9.1 PA6 PAD20 PAS50 Pirate Procrass1 QueenBey Rileysaurus SKKY Solid Stormborn Supernova Wizzo |
| Annotating Institution | Unknown or unassigned |
| Annotation Status | In GenBank |
| Plaque Notes | Clear plaques with turbid centers. Size range between 2-5mm, average 3 mm in diameter. |
| Morphotype | Siphoviridae |
| Number of Genes | 44 |
| Number of tRNAs | 0 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
Click to ViewMEAK_1 MEAK_2 MEAK_3 MEAK_4 MEAK_5 MEAK_6 MEAK_7 MEAK_8 MEAK_9 MEAK_10 MEAK_11 MEAK_12 MEAK_13 MEAK_14 MEAK_15 MEAK_16 MEAK_17 MEAK_18 MEAK_19 MEAK_20 MEAK_21 MEAK_22 MEAK_23 MEAK_24 MEAK_25 MEAK_26 MEAK_27 MEAK_28 MEAK_29 MEAK_30 MEAK_31 MEAK_32 MEAK_33 MEAK_34 MEAK_35 MEAK_36 MEAK_37 MEAK_38 MEAK_39 MEAK_40 MEAK_41 MEAK_42 MEAK_43 MEAK_44 |
| Submitted Minimal DNA Master File | Download |
| Publication Info | |
| Uploaded to GenBank? | Yes |
| GenBank Accession | MF919522 |
| Refseq Number | None yet |
| Archiving Info | |
| Archiving status | Archived |
| Pitt Freezer Box# | 28 |
| Pitt Freezer Box Grid# | D12 |
| Available Files | |
| Plaque Picture | Download |
| EM Picture | Download |
| GenBank File for Phamerator | Download |