| Discovery Information |
| Isolation Host | Streptomyces sanglieri UNT16F27A |
| Found By | Marilyn Jimenez and Darla Benavides |
| Year Found | 2019 |
| Location Found | Denton, TX United States |
| Finding Institution | University of North Texas |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | No |
| Isolation Temperature | 30°C |
| GPS Coordinates | 33.209228 N, 97.147939 W Map |
| Discovery Notes | I collected my Soil sample beside the Business leadership Building at UNT campus underneath a tree. Soil was beside minimal amount of grass and it was dry. I dug down about 2cm and then collected one- fourth of a bag of soil. Soil was a mix of dead wood. The soil had a crumbly texture. Overall the color was medium brown. The outside temperature was 86 degrees Farenheit. The sample was collected on August 26, 2019 at 11pm at night. |
| Naming Notes | I named this based on my first and last name. My first name is Marilyn so I used the first three letters. My last name is Jimenez I used the first three letters of my last name, putting this together it creates Marsus. |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Jan 10, 2025 |
| Sequencing Facility | University of North Texas |
| Shotgun Sequencing Method | Illumina |
| Sequencer Used | Illumina MiSeq |
| Read Type | Single-end reads |
| Read Length | 150 bp |
| Approximate Shotgun Coverage | 53 |
| Genome length (bp) | 134686 |
| Character of genome ends | Direct Terminal Repeat |
| Direct Terminal Repeat Length | 10982 bp |
| GC Content | 49.5% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | BE |
| Subcluster | BE1 |
| Cluster Life Cycle | Lytic |
| Lysogeny Notes | We performed a Spot Test on Marsus, this plate was incubated for a longer period than 48 hours. The results did not create a masa therefore we did not proceed to do the lysogen test. |
| Other Cluster Members |
|
| Annotating Institution | University of North Texas |
| Annotation Status | In GenBank |
| Plaque Notes | This plaque has alway been approximately 1mm or less. It has been clear and circular in shape. |
| Number of Genes | 238 |
| Number of tRNAs | 40 |
| Number of tmRNAs | 1 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Minimal DNA Master File | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | PV915847 |
| Refseq Number | None yet |
| Archiving Info |
| Archiving status |
Archived |
| SEA Lysate Titer |
1.4X10^8 |
| Pitt Freezer Box# |
104 |
| Pitt Freezer Box Grid# |
C3 |
| Available Files |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| GenBank File for Phamerator | Download |
