| Discovery Information |
| Isolation Host | Streptomyces mirabilis NRRL B-2400 |
| Found By | Jenell Lewis and Hira Ahmed |
| Year Found | 2019 |
| Location Found | Poolesville, MD United States |
| Finding Institution | University of Maryland, Baltimore County |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | No |
| Isolation Temperature | 30°C |
| GPS Coordinates | 39.129923 N, 77.411686 W Map |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Apr 20, 2020 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina |
| Sequencer Used | Illumina MiSeq |
| Read Type | Single-end reads |
| Read Length | 150 bp |
| Approximate Shotgun Coverage | 590 |
| Genome length (bp) | 130258 |
| Character of genome ends | Direct Terminal Repeat |
| Direct Terminal Repeat Length | 12198 bp |
| GC Content | 49.5% |
| Sequencing Notes | When this phage was sequenced, there was also a smaller "phagelet" in the sample at much higher read depth than this phage. The phagelet was named MiniFlayer. MindFlayer was subsequently separated from MiniFlayer in the lab. |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | BE |
| Subcluster | BE2 |
| Cluster Life Cycle | Lytic |
| Lysogeny Notes | Most likely virulent. |
| Other Cluster Members |
|
| Annotating Institution | University of Maryland, Baltimore County |
| Annotation Status | In GenBank |
| Plaque Notes | Clear plaques, varying sizes of 1mm to 5mm, and small plaques are round while larger plaques are irregular in shape. |
| Morphotype | Siphoviridae |
| Number of Genes | 234 |
| Number of tRNAs | 42 |
| Number of tmRNAs | 1 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Minimal DNA Master File | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | MW291014 |
| Refseq Number | None yet |
| Published in Paper | deCarvalho et al, 2023 2023, The ISME Journal: Simultaneous entry as an adaptation to virulence in a novel satellite-helper system infecting Streptomyces species |
| Archiving Info |
| Archiving status |
Archived |
| SEA Lysate Titer |
8.1e8 |
| Pitt Freezer Box# |
117 |
| Pitt Freezer Box Grid# |
A8 |
| Available Files |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| EM Picture | Download |
| GenBank File for Phamerator | Download |
