Streptomyces phage Mugiwara
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Detailed Information for Phage Mugiwara
Discovery Information
Isolation HostStreptomyces avermitilis NRRL 8165
Found ByGil Toex
Year Found2023
Location FoundNoertrange, Luxembourg
Finding InstitutionUniversity of Ottawa
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?No
Isolation Temperature26°C
GPS Coordinates49.98127 N, 5.903158 E Map
Discovery NotesFound in a grassy area near fir trees (3-5 cm below the surface).
Genomic DNA was sequenced on a ONT Minion, R10.4.1 chemistry. The sequence assembled into 5 contigs. Two contigs (3,047bp and 2,300pb) with an average of 81.33% query cover, 89.84% indentity to phiScoe45 which is 88.68% identical to Saftant (BD3). Two contigs (2,785bp and 2,589bp) with an average of 95.66% query cover, 89.24% indentity to LukeCage (BE2). One contig (2,564bp) with 65% query cover, 92.04% indentity to Endor2 which is 93.68% indentical to Celery (BD3).
Naming NotesThe name "Mugiwara" comes from a Japanese anime called "One Piece" and means "straw hat".
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedJan 6, 2024
Sequencing FacilityPittsburgh Bacteriophage Institute
Shotgun Sequencing MethodIllumina
Approximate Shotgun Coverage391
Genome length (bp)133330
Character of genome endsDirect Terminal Repeat
Direct Terminal Repeat Length11945 bp
GC Content49.1%
Fasta file available?Yes: Download fasta file
Characterization
ClusterBE
SubclusterBE2
Cluster Life CycleLytic
Other Cluster Members
Annotating InstitutionUnknown or unassigned
Annotation StatusPlanless
Plaque NotesSmall and clear plaques
MorphotypeSiphoviridae
Number of Genes255
Number of tRNAs42
Number of tmRNAs1
Has been Phamerated?Yes
Gene List

Click to View

Mugiwara_1

Mugiwara_2

Mugiwara_3

Mugiwara_4

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Mugiwara_6

Mugiwara_7

Mugiwara_8

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Mugiwara_11

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Mugiwara_13

Mugiwara_14

Mugiwara_15

Mugiwara_16

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Mugiwara_23

Mugiwara_24

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Mugiwara_32

Mugiwara_33

Mugiwara_34

Mugiwara_35

Mugiwara_36

Mugiwara_37

Mugiwara_38

Mugiwara_39 — tRNA
(gp39)

Mugiwara_40

Mugiwara_41

Mugiwara_42

Mugiwara_43

Mugiwara_44

Mugiwara_45

Mugiwara_46

Mugiwara_47

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Mugiwara_53

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Mugiwara_63

Mugiwara_64

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Mugiwara_75

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Mugiwara_95

Mugiwara_96

Mugiwara_97

Mugiwara_98

Mugiwara_99

Mugiwara_100

Mugiwara_101

Mugiwara_102

Mugiwara_103

Mugiwara_104

Mugiwara_105 — tRNA
(gp105)

Mugiwara_106 — tRNA
(gp106)

Mugiwara_107 — tRNA
(gp107)

Mugiwara_108

Mugiwara_109

Mugiwara_110

Mugiwara_111 — tRNA
(gp111)

Mugiwara_112 — tRNA
(gp112)

Mugiwara_113

Mugiwara_114

Mugiwara_115

Mugiwara_116 — tRNA
(gp116)

Mugiwara_117

Mugiwara_118 — tRNA
(gp118)

Mugiwara_119

Mugiwara_120

Mugiwara_121 — tRNA
(gp121)

Mugiwara_122

Mugiwara_123 — tRNA
(gp123)

Mugiwara_124

Mugiwara_125

Mugiwara_126

Mugiwara_127

Mugiwara_128

Mugiwara_129 — tRNA
(gp129)

Mugiwara_130

Mugiwara_131

Mugiwara_132

Mugiwara_133 — tRNA
(gp133)

Mugiwara_134

Mugiwara_135

Mugiwara_136

Mugiwara_137

Mugiwara_138

Mugiwara_139

Mugiwara_140

Mugiwara_141

Mugiwara_142

Mugiwara_143

Mugiwara_144

Mugiwara_145

Mugiwara_146

Mugiwara_147 — tRNA
(gp147)

Mugiwara_148

Mugiwara_149

Mugiwara_150 — tRNA
(gp150)

Mugiwara_151 — tRNA
(gp151)

Mugiwara_152 — tRNA
(gp152)

Mugiwara_153 — tRNA
(gp153)

Mugiwara_154 — tRNA
(gp154)

Mugiwara_155 — tRNA
(gp155)

Mugiwara_156 — tRNA
(gp156)

Mugiwara_157

Mugiwara_158

Mugiwara_159

Mugiwara_160

Mugiwara_161 — tRNA
(gp161)

Mugiwara_162 — tRNA
(gp162)

Mugiwara_163

Mugiwara_164

Mugiwara_165 — tRNA
(gp165)

Mugiwara_166

Mugiwara_167 — tRNA
(gp167)

Mugiwara_168

Mugiwara_169

Mugiwara_170

Mugiwara_171 — tRNA
(gp171)

Mugiwara_172

Mugiwara_173 — tRNA
(gp173)

Mugiwara_174 — tRNA
(gp174)

Mugiwara_175 — tRNA
(gp175)

Mugiwara_176

Mugiwara_177 — tRNA
(gp177)

Mugiwara_178

Mugiwara_179

Mugiwara_180 — tRNA
(gp180)

Mugiwara_181

Mugiwara_182 — tRNA
(gp182)

Mugiwara_183 — tRNA
(gp183)

Mugiwara_184

Mugiwara_185

Mugiwara_186 — tRNA
(gp186)

Mugiwara_187

Mugiwara_188

Mugiwara_189

Mugiwara_190 — tRNA
(gp190)

Mugiwara_191 — tRNA
(gp191)

Mugiwara_192 — tRNA
(gp192)

Mugiwara_193

Mugiwara_194 — tRNA
(gp194)

Mugiwara_195

Mugiwara_196

Mugiwara_197

Mugiwara_198

Mugiwara_199

Mugiwara_200 — tmRNA
(gp200)

Mugiwara_201

Mugiwara_202

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Mugiwara_204

Mugiwara_205

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Mugiwara_207

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Mugiwara_217

Mugiwara_218

Mugiwara_219

Mugiwara_220

Mugiwara_221

Mugiwara_222

Mugiwara_223

Mugiwara_224 — tRNA
(gp224)

Mugiwara_225

Mugiwara_226

Mugiwara_227 — tRNA
(gp227)

Mugiwara_228

Mugiwara_229

Mugiwara_230

Mugiwara_231 — tRNA
(gp231)

Mugiwara_232

Mugiwara_233 — tRNA
(gp233)

Mugiwara_234

Mugiwara_235

Mugiwara_236

Mugiwara_237

Mugiwara_238

Mugiwara_239

Mugiwara_240

Mugiwara_241

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Mugiwara_243

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Mugiwara_249

Mugiwara_250

Mugiwara_251

Mugiwara_252

Mugiwara_253

Mugiwara_254

Mugiwara_255

Mugiwara_256

Mugiwara_257

Mugiwara_258 — tRNA
(gp258)

Mugiwara_259

Mugiwara_260

Mugiwara_261

Mugiwara_262

Mugiwara_263

Mugiwara_264

Mugiwara_265

Mugiwara_266

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Mugiwara_291

Mugiwara_292

Mugiwara_293

Mugiwara_294

Mugiwara_295

Mugiwara_296

Mugiwara_297

Mugiwara_298

Publication Info
Uploaded to GenBank?No
GenBank AccessionNone yet
Refseq NumberNone yet
Archiving Info
Archiving status Archived
SEA Lysate Titer 1.4 X 10^9
Pitt Freezer Box# 176
Pitt Freezer Box Grid# E12
Available Files
Plaque PictureDownload
Restriction Digest PictureDownload
EM PictureDownload