| Discovery Information |
| Isolation Host | Microbacterium foliorum NRRL B-24224 |
| Found By | Andrew Tinman & Carly Brod |
| Year Found | 2020 |
| Location Found | Frederic, WI USA |
| Finding Institution | University of Wisconsin-River Falls |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | 46°C |
| GPS Coordinates | 45.6535 N, 92.4671 W Map |
| Discovery Notes | The soil was dug up behind an old fishing store that would regularly dump organic waste in the same spot for 50 years, creating a lush little grove behind the store. The temperature was 46 degrees Fahrenheit when it was collected. |
| Naming Notes | The old fishing store that dumped the organic waste into the little grove raised their own minnows. So when some died, plus the waste left by the fish that were sold would be dumped into the small grove behind the store for 50 years, making the soil more and more fertile over time. So I looked up what the scientific name of Minnow was and it is Phoxinus. So I came up with the name Necrophoxinus, which means Dead Minnow. |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Jan 26, 2021 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina |
| Approximate Shotgun Coverage | 376 |
| Genome length (bp) | 64175 |
| Character of genome ends | Direct Terminal Repeat |
| Direct Terminal Repeat Length | 3932 bp |
| GC Content | 61.7% |
| Sequencing Notes | There were two deletions in subpopulations seen in the sequencing reads for this genome. The first was a 424 bp deletion from position 309 to 732 (inclusive) present in ~35% of reads. The second, also present in ~35% of reads, is 90 bp from position 850 to 939, inclusive. |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | ED |
| Subcluster | ED2 |
| Cluster Life Cycle | Lytic |
| Other Cluster Members |
|
| Annotating Institution | University of Wisconsin-River Falls |
| Annotation Status | In GenBank |
| Plaque Notes | Our phage that we singled out is a medium phage that has a halo and that we believe it has a lytic cycle. This is because of the size of the plaque and that they are clear. We also believe that there is only one kind of phage due to the uniform morphology of the plaques. |
| Morphotype | Siphoviridae |
| Number of Genes | 123 |
| Number of tRNAs | 3 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Minimal DNA Master File | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | MZ355724 |
| Refseq Number | None yet |
| Archiving Info |
| Archiving status |
Archived |
| Pitt Freezer Box# |
127 |
| Pitt Freezer Box Grid# |
G10 |
| Available Files |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| EM Picture | Download |
| GenBank File for Phamerator | Download |
