| Detailed Information for Phage Ouroboros | |
| Discovery Information | |
| Isolation Host | Propionibacterium acnes ATCC 6919 |
| Found By | Josh Chiou |
| Year Found | 2013 |
| Location Found | Los Angeles, CA USA |
| Finding Institution | University of California, Los Angeles |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | No |
| Isolation Temperature | 37°C |
| GPS Coordinates | 34.065 N, 118.4425 W Map |
| Discovery Notes | Microcomedones from Josh Chiou’s nose (washed face, no antibiotics used, normal skin) were extracted using a Biore Pore Strip on October 4th, 2013, and the sample was enriched in RCM media with P. acnes strain ATCC 6919 under anaerobic conditions at 37 oC. Phage Ouroboros was isolated, characterized and annotated by Team Hannibal Vector (Payton Smithers, Natalie Liu, Jay Gong, Victoria Chen). TA: Samantha Capati. Instructor: Dr. Jordan Moberg Parker. Laboratory Support: Dr. Krisanavane Reddi. Assembly performed by Will Villella (UCLA) and confirmed by Charles Bowman (University of Pittsburgh). Quality control of annotation results performed by Jordan Moberg Parker and Dallas Mould (UCLA). |
| Sequencing Information | |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Apr 24, 2014 |
| Sequencing Facility | The UCLA Genotyping and Sequencing Core |
| Shotgun Sequencing Method | Illumina Sequencing |
| Approximate Shotgun Coverage | 1000 |
| Genome length (bp) | 29506 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 11 bases |
| Overhang Sequence | TCGTACGGCTT |
| GC Content | 53.9% |
| Fasta file available? | Yes: Download fasta file |
| Characterization | |
| Cluster | BU |
| Subcluster | -- |
| Cluster Life Cycle | Lytic |
| Other Cluster Members |
Click to ViewAquarius ATCC29399BC ATCC29399BT Attacne BruceLethal Cota DrParker Enochoraptor Enoki Keiki Kubed Lauchelly Leviosa LilBandit MEAK Moyashi MrAK Ouroboros P1.1 P100.1 P100A P100D P101A P104A P104B P105 P106A P106C P106I P106L P106M P107A P107C P108C P14.4 P9.1 PA6 PAD20 PAS50 Pirate Procrass1 QueenBey Rileysaurus SKKY Solid Stormborn Supernova Wizzo |
| Annotating Institution | Unknown or unassigned |
| Annotation Status | In GenBank |
| Plaque Notes | Plaques were clear, round and ranged from 4.4-5.6 mm in diameter at lower titers. Later phage preparations at higher titers produced slightly turbid plaques 8.4 mm in diameter. |
| Morphotype | Siphoviridae |
| Number of Genes | 45 |
| Number of tRNAs | 0 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
Click to ViewOuroboros_1 Ouroboros_2 Ouroboros_3 Ouroboros_4 Ouroboros_5 Ouroboros_6 Ouroboros_7 Ouroboros_8 Ouroboros_9 Ouroboros_10 Ouroboros_11 Ouroboros_12 Ouroboros_13 Ouroboros_14 Ouroboros_15 Ouroboros_16 Ouroboros_17 Ouroboros_18 Ouroboros_19 Ouroboros_20 Ouroboros_21 Ouroboros_22 Ouroboros_23 Ouroboros_24 Ouroboros_25 Ouroboros_26 Ouroboros_27 Ouroboros_28 Ouroboros_29 Ouroboros_30 Ouroboros_31 Ouroboros_32 Ouroboros_33 Ouroboros_34 Ouroboros_35 Ouroboros_36 Ouroboros_37 Ouroboros_38 Ouroboros_39 Ouroboros_40 Ouroboros_41 Ouroboros_42 Ouroboros_43 Ouroboros_44 Ouroboros_45 |
| Publication Info | |
| Uploaded to GenBank? | Yes |
| GenBank Accession | KR337654 |
| Refseq Number | None yet |
| Archiving Info | |
| Archiving status | Archived |
| Pitt Freezer Box# | 23 |
| Pitt Freezer Box Grid# | C7 |
| Available Files | |
| Plaque Picture | Download |
| EM Picture | Download |
| Final DNAMaster File | Download |