| Detailed Information for Phage Pepperwood |
| Discovery Information |
| Isolation Host | Streptomyces lividans JI 1326 |
| Found By | Anushka Viswanathan, Shreya Chilukuri |
| Year Found | 2021 |
| Location Found | Pittsburg, MO United States |
| Finding Institution | Washington University in St. Louis |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | 10°C |
| GPS Coordinates | 37.878231 N, 93.324399 W Map |
| Discovery Notes | The soil was collected at 3 cm. |
| Naming Notes | Named after Pepperwood Chronicles from a TV show that parallels our journey in isolating this phage. |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Feb 7, 2022 |
| Sequencing Facility | The McDonnell Genome Institute at Washington University |
| Shotgun Sequencing Method | Illumina |
| Sequencer Used | Illumina MiSeq |
| Read Type | Single-end reads |
| Read Length | 150 bp |
| Approximate Shotgun Coverage | 153 |
| Genome length (bp) | 133012 |
| Character of genome ends | Direct Terminal Repeat |
| Direct Terminal Repeat Length | 11227 bp |
| GC Content | 50.1% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | BE |
| Subcluster | BE1 |
| Cluster Life Cycle | Lytic |
| Lysogeny Notes | Majority of the plaques were clear so we concluded that Pepperwood was in the lytic cycle. |
| Other Cluster Members |
|
| Annotating Institution | Washington University in St. Louis |
| Annotation Status | In GenBank |
| Plaque Notes | There were numerous small, circular plaques with well-defined edges. |
| Morphotype | Siphoviridae |
| Number of Genes | 229 |
| Number of tRNAs | 43 |
| Number of tmRNAs | 1 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Minimal DNA Master File | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | OQ190480 |
| Refseq Number | None yet |
| Archiving Info |
| Archiving status |
Not in Pitt Archives |
| SEA Lysate Titer |
2.42x10^10 pfu/mL |
| Available Files |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| EM Picture | Download |
| GenBank File for Phamerator | Download |
