| Detailed Information for Phage Piccoletto |
| Discovery Information |
| Isolation Host | Arthrobacter sp. ATCC 21022 |
| Duplicates of this Phage | Nahla |
| Found By | Brianna Sanchez and Meygan Martinez |
| Year Found | 2015 |
| Location Found | Cloudcroft, NM USA |
| Finding Institution | University of Texas at El Paso |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | 35°C |
| GPS Coordinates | 32.829367 N, 105.739117 W Map |
| Discovery Notes | This is the same soil sample used to isolate Arthrobacteriophage Nahla. The sample was collected on a cloudy day at around 1:00 PM. The elevation of the place where the sample was collected is 2,518 m above sea level. The sample was collected at the root of a mature pine. |
| Naming Notes | The original name assigned by the students was "Piccolo" and that was the name used for initial submission for sequencing. However, we were informed that the name had already been assigned to another phage. Therefore, one of the instructors (Dr. Rosas-Acosta) modified the name to "Piccoletto", which in Italian means "short person" |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Jan 29, 2016 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Genome length (bp) | 49808 |
| Character of genome ends | Unknown |
| GC Content | 63.6% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | AO |
| Subcluster | AO1 |
| Cluster Life Cycle | Lytic |
| Other Cluster Members |
|
| Annotating Institution | Unknown or unassigned |
| Annotation Status | In GenBank |
| Plaque Notes | Picolletto produces medium-size clear plaques of about 2.425 mm in diameter when grown in an Arthrobacter sp. host at 35oC for about 16-18 hours. |
| Morphotype | Myoviridae |
| Number of Genes | 74 |
| Number of tRNAs | 0 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Annotation in DNA Master Format | Download |
| Submitted Minimal DNA Master File | Download |
| Author List | Download |
| Annotation Cover Sheet | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | MF189177 |
| Refseq Number | None yet |
| Archiving Info |
| Archiving status |
Archived |
| Pitt Freezer Box# |
26 |
| Pitt Freezer Box Grid# |
F8 |
| Available Files |
| Plaque Picture | Download |
| EM Picture | Download |
| Final DNAMaster File | Download |
| GenBank File for Phamerator | Download |
