| Detailed Information for Phage PinkiePie |
| Discovery Information |
| Isolation Host | Streptomyces lividans JI 1326 |
| Found By | Maeve Repking, Sarah Zhang, Anna Liu |
| Year Found | 2022 |
| Location Found | St. Louis, MO USA |
| Finding Institution | Washington University in St. Louis |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | No |
| Isolation Temperature | Not entered |
| GPS Coordinates | 38.643746 N, 90.298535 W Map |
| Discovery Notes | Our phage was found in Forest Park right next to the Cascades. The soil was collected 6.0cm below surface where the soil was wet, organic matter. The temperature during collection was 23 degrees C. |
| Naming Notes | Our plates often turned pink, so we chose the name PinkiePie, which is a character from the children’s TV show My Little Pony. |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Feb 16, 2023 |
| Sequencing Facility | The McDonnell Genome Institute at Washington University |
| Shotgun Sequencing Method | Illumina |
| Approximate Shotgun Coverage | 154 |
| Genome length (bp) | 132301 |
| Character of genome ends | Direct Terminal Repeat |
| Direct Terminal Repeat Length | 10780 bp |
| GC Content | 50.2% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | BE |
| Subcluster | BE1 |
| Cluster Life Cycle | Lytic |
| Other Cluster Members |
|
| Annotating Institution | Washington University in St. Louis |
| Annotation Status | In GenBank |
| Number of Genes | 231 |
| Number of tRNAs | 40 |
| Number of tmRNAs | 1 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Minimal DNA Master File | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | OR253911 |
| Refseq Number | None yet |
| Archiving Info |
| Archiving status |
Not in Pitt Archives |
| Available Files |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| EM Picture | Download |
| GenBank File for Phamerator | Download |
