| Detailed Information for Phage Preamble |
| Discovery Information |
| Isolation Host | Arthrobacter sp. ATCC 21022 |
| Found By | Dylan Chudoff |
| Year Found | 2014 |
| Location Found | Radnor, PA USA |
| Finding Institution | Cabrini University |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | Not entered |
| GPS Coordinates | 39.913709 N, 74.902875 W Map |
| Discovery Notes | I took the soil from a hole that was dug by my dog under the fence. This hole accumulates a good amount of water and usually makes a puddle when it rains. There was also a high proportion of organic matter in my sample.
Please note that phage lysate was made with glycerol for archiving purposes.
|
| Naming Notes | This phage is named after the street where the soil sample was collected. |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Nov 13, 2014 |
| Sequencing Facility | NC State Genomic Sciences Laboratory |
| Shotgun Sequencing Method | Illumina |
| Sequencer Used | Illumina MiSeq |
| Read Type | Single-end reads |
| Read Length | 150 bp |
| Approximate Shotgun Coverage | 1308 |
| Genome length (bp) | 43374 |
| Character of genome ends | 3' Sticky Overhang |
| Overhang Length | 13 bases |
| Overhang Sequence | GGTAACCGTGATA |
| GC Content | 60.7% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | AK |
| Subcluster | -- |
| Cluster Life Cycle | Lytic |
| Lysogeny Notes | Lytic |
| Other Cluster Members |
|
| Annotating Institution | Unknown or unassigned |
| Annotation Status | In GenBank |
| Plaque Notes | Plaques are average in size, and do not "bulls-eye". |
| Morphotype | Siphoviridae |
| Number of Genes | 64 |
| Number of tRNAs | 0 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Annotation in DNA Master Format | Download |
| Submitted Minimal DNA Master File | Download |
| Author List | Download |
| Annotation Cover Sheet | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | KU160659 |
| Refseq Number | None yet |
| Archiving Info |
| Archiving status |
Archived |
| SEA Lysate Titer |
greater than 10 to the tenth |
| Date of SEA Lysate Titering |
Mar 15, 2015 |
| Pitt Freezer Box# |
149 |
| Pitt Freezer Box Grid# |
A12 |
| Available Files |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| EM Picture | Download |
| Final DNAMaster File | Download |
