Streptomyces phage PumpkinSpice
PumpkinSpice Plaque Picture
PumpkinSpice EM Picture
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Detailed Information for Phage PumpkinSpice
Discovery Information
Isolation HostStreptomyces griseofuscus ATCC 23916
Found ByBrandon Chia & Paul Rice
Year Found2017
Location FoundSt. Louis, MO USA
Finding InstitutionWashington University in St. Louis
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?No
Isolation Temperature34°C
GPS Coordinates38.6457 N, 90.3148 W Map
Discovery NotesObtained from underneath a tree outside of the Nathan Dardick House. Taken from surface level, <1cm, with an ambient temperature of 93 degrees Fahrenheit
Naming NotesIt's fall.
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedFeb 13, 2018
Sequencing FacilityThe McDonnell Genome Institute at Washington University
Shotgun Sequencing MethodIllumina
Approximate Shotgun Coverage85
Genome length (bp)132480
Character of genome endsDirect Terminal Repeat
Direct Terminal Repeat Length12588 bp
GC Content49.4%
Fasta file available?Yes: Download fasta file
Characterization
ClusterBE
SubclusterBE2
Cluster Life CycleLytic
Other Cluster Members
Annotating InstitutionUniversity of California, Los Angeles
Annotation StatusIn GenBank
Plaque NotesThe plaque formed were of varying size although overall small. Plaques were clear and had relatively defined edges.
MorphotypeSiphoviridae
Number of Genes246
Number of tRNAs44
Number of tmRNAs1
Has been Phamerated?Yes
Gene List

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PumpkinSpice_1

PumpkinSpice_2

PumpkinSpice_3

PumpkinSpice_4

PumpkinSpice_5

PumpkinSpice_6

PumpkinSpice_7
(Lsr2-like DNA bridging protein)

PumpkinSpice_8

PumpkinSpice_9

PumpkinSpice_10

PumpkinSpice_11

PumpkinSpice_12

PumpkinSpice_13
(ParB-like nuclease domain protein)

PumpkinSpice_14

PumpkinSpice_15

PumpkinSpice_16

PumpkinSpice_17

PumpkinSpice_18

PumpkinSpice_19

PumpkinSpice_20

PumpkinSpice_21

PumpkinSpice_22

PumpkinSpice_23

PumpkinSpice_24

PumpkinSpice_25

PumpkinSpice_26

PumpkinSpice_27
(HNH endonuclease)

PumpkinSpice_28

PumpkinSpice_29
(ThyX-like thymidylate synthase)

PumpkinSpice_30

PumpkinSpice_31

PumpkinSpice_32

PumpkinSpice_33

PumpkinSpice_34
(DNA binding protein)

PumpkinSpice_35

PumpkinSpice_36

PumpkinSpice_37

PumpkinSpice_38 — tRNA
(tRNA-Thr(cgt))

PumpkinSpice_39
(HNH endonuclease)

PumpkinSpice_40
(transglycosylase)

PumpkinSpice_41

PumpkinSpice_42

PumpkinSpice_43
(glycosyltransferase)

PumpkinSpice_44
(ribonucleotide reductase)

PumpkinSpice_45
(endolysin)

PumpkinSpice_46

PumpkinSpice_47

PumpkinSpice_48
(portal protein)

PumpkinSpice_49

PumpkinSpice_50
(capsid maturation protease)

PumpkinSpice_51
(major capsid protein)

PumpkinSpice_52

PumpkinSpice_53
(head-to-tail adaptor)

PumpkinSpice_54
(head-to-tail stopper)

PumpkinSpice_55

PumpkinSpice_56

PumpkinSpice_57
(major tail protein)

PumpkinSpice_58
(tail assembly chaperone)

PumpkinSpice_59
(tail assembly chaperone)

PumpkinSpice_60
(tape measure protein)

PumpkinSpice_61
(minor tail protein)

PumpkinSpice_62
(minor tail protein)

PumpkinSpice_63

PumpkinSpice_64
(minor tail protein)

PumpkinSpice_65
(minor tail protein)

PumpkinSpice_66

PumpkinSpice_67

PumpkinSpice_68

PumpkinSpice_69
(HNH endonuclease)

PumpkinSpice_70
(phosphoesterase)

PumpkinSpice_71
(holin)

PumpkinSpice_72
(helix-turn-helix DNA binding domain protein)

PumpkinSpice_73
(helix-turn-helix DNA binding domain protein)

PumpkinSpice_74

PumpkinSpice_75

PumpkinSpice_76

PumpkinSpice_77
(DnaB-like dsDNA helicase)

PumpkinSpice_78

PumpkinSpice_79
(DNA primase)

PumpkinSpice_80
(SSB protein)

PumpkinSpice_81
(DnaE-like DNA polymerase III alpha)

PumpkinSpice_82

PumpkinSpice_83
(MazG-like nucleotide pyrophosphohydrolase)

PumpkinSpice_84
(RecA-like DNA recombinase)

PumpkinSpice_85

PumpkinSpice_86
(holliday junction resolvase)

PumpkinSpice_87

PumpkinSpice_88

PumpkinSpice_89

PumpkinSpice_90
(Cas4 family exonuclease)

PumpkinSpice_91

PumpkinSpice_92
(RuvC-like resolvase)

PumpkinSpice_93
(DprA-like DNA processing chain A)

PumpkinSpice_94
(helix-turn-helix DNA binding domain protein)

PumpkinSpice_95
(methyltransferase)

PumpkinSpice_96
(thymidylate kinase)

PumpkinSpice_97

PumpkinSpice_98

PumpkinSpice_99
(terminase large subunit)

PumpkinSpice_100
(HNH endonuclease)

PumpkinSpice_101

PumpkinSpice_102 — tRNA
(tRNA-Gly(tcc))

PumpkinSpice_103 — tRNA
(tRNA-Gln(ctg))

PumpkinSpice_104 — tRNA
(tRNA-Trp(cca))

PumpkinSpice_105

PumpkinSpice_106

PumpkinSpice_107 — tRNA
(tRNA-Pro(tgg))

PumpkinSpice_108
(HNH endonuclease)

PumpkinSpice_109 — tRNA
(tRNA-Pro(tgg))

PumpkinSpice_110
(nucleotidyl transferase)

PumpkinSpice_111
(FtsK-like DNA translocase)

PumpkinSpice_112

PumpkinSpice_113 — tRNA
(tRNA-Ile(tat))

PumpkinSpice_114

PumpkinSpice_115 — tRNA
(tRNA-Leu(taa))

PumpkinSpice_116

PumpkinSpice_117

PumpkinSpice_118 — tRNA
(tRNA-Ser(gga))

PumpkinSpice_119

PumpkinSpice_120 — tRNA
(tRNA-Ser(tga))

PumpkinSpice_121

PumpkinSpice_122

PumpkinSpice_123

PumpkinSpice_124

PumpkinSpice_125

PumpkinSpice_126

PumpkinSpice_127 — tRNA
(tRNA-Asp(gtc))

PumpkinSpice_128

PumpkinSpice_129

PumpkinSpice_130 — tRNA
(tRNA-Asp(gtc))

PumpkinSpice_131

PumpkinSpice_132
(Ro-like RNA binding protein)

PumpkinSpice_133

PumpkinSpice_134

PumpkinSpice_135

PumpkinSpice_136

PumpkinSpice_137
(helix-turn-helix DNA binding domain protein)

PumpkinSpice_138

PumpkinSpice_139

PumpkinSpice_140

PumpkinSpice_141

PumpkinSpice_142

PumpkinSpice_143 — tRNA
(tRNA-Glu(ctc))

PumpkinSpice_144

PumpkinSpice_145 — tRNA
(tRNA-Glu(ttc))

PumpkinSpice_146 — tRNA
(tRNA-Val(tac))

PumpkinSpice_147 — tRNA
(tRNA-Leu(tag))

PumpkinSpice_148 — tRNA
(tRNA-Leu(tag))

PumpkinSpice_149 — tRNA
(tRNA-Phe(gaa))

PumpkinSpice_150
(HNH endonuclease)

PumpkinSpice_151 — tRNA
(tRNA-Gly(gcc))

PumpkinSpice_152 — tRNA
(tRNA-Arg(tct))

PumpkinSpice_153

PumpkinSpice_154

PumpkinSpice_155

PumpkinSpice_156 — tRNA
(tRNA-Ala(tgc))

PumpkinSpice_157 — tRNA
(tRNA-Lys(ctt))

PumpkinSpice_158
(peptidase)

PumpkinSpice_159

PumpkinSpice_160 — tRNA
(tRNA-Asn(gtt))

PumpkinSpice_161

PumpkinSpice_162

PumpkinSpice_163

PumpkinSpice_164 — tRNA
(tRNA-Asn(gtt))

PumpkinSpice_165
(band-7-like membrane protein)

PumpkinSpice_166

PumpkinSpice_167

PumpkinSpice_168 — tRNA
(tRNA-Arg(cct))

PumpkinSpice_169 — tRNA
(tRNA-Ala(ggc))

PumpkinSpice_170 — tRNA
(tRNA-Lys(ctt))

PumpkinSpice_171 — tRNA
(tRNA-Lys(ctt))

PumpkinSpice_172

PumpkinSpice_173 — tRNA
(tRNA-Lys(ttt))

PumpkinSpice_174

PumpkinSpice_175 — tRNA
(tRNA-Arg(tcg))

PumpkinSpice_176

PumpkinSpice_177 — tRNA
(tRNA-Met(cat))

PumpkinSpice_178 — tRNA
(tRNA-Met(cat))

PumpkinSpice_179

PumpkinSpice_180

PumpkinSpice_181 — tRNA
(tRNA-Tyr(gta))

PumpkinSpice_182 — tRNA
(tRNA-His(gtg))

PumpkinSpice_183

PumpkinSpice_184

PumpkinSpice_185

PumpkinSpice_186 — tRNA
(tRNA-Cys(gca))

PumpkinSpice_187 — tRNA
(tRNA-Thr(tgt))

PumpkinSpice_188 — tRNA
(tRNA-Thr(ggt))

PumpkinSpice_189

PumpkinSpice_190 — tRNA
(tRNA-Arg(acg))

PumpkinSpice_191

PumpkinSpice_192
(DnaE-like DNA polymerase III alpha)

PumpkinSpice_193

PumpkinSpice_194 — tmRNA
(Tag peptide: ANSNVASAYALAA*)

PumpkinSpice_195

PumpkinSpice_196

PumpkinSpice_197
(acetyltransferase)

PumpkinSpice_198

PumpkinSpice_199

PumpkinSpice_200 — tRNA
(tRNA-?(Ala|Gly)(gc))

PumpkinSpice_201

PumpkinSpice_202

PumpkinSpice_203

PumpkinSpice_204

PumpkinSpice_205

PumpkinSpice_206
(RNA ligase)

PumpkinSpice_207

PumpkinSpice_208

PumpkinSpice_209

PumpkinSpice_210

PumpkinSpice_211
(polynucleotide kinase)

PumpkinSpice_212

PumpkinSpice_213

PumpkinSpice_214

PumpkinSpice_215 — tRNA
(tRNA-Leu(gag))

PumpkinSpice_216

PumpkinSpice_217

PumpkinSpice_218 — tRNA
(tRNA-Val(gac))

PumpkinSpice_219
(SprT-like protease)

PumpkinSpice_220

PumpkinSpice_221

PumpkinSpice_222

PumpkinSpice_223 — tRNA
(tRNA-Leu(caa))

PumpkinSpice_224

PumpkinSpice_225 — tRNA
(tRNA-Ser(gct))

PumpkinSpice_226

PumpkinSpice_227

PumpkinSpice_228

PumpkinSpice_229

PumpkinSpice_230

PumpkinSpice_231

PumpkinSpice_232

PumpkinSpice_233

PumpkinSpice_234
(deoxycytidylate deaminase)

PumpkinSpice_235
(phosphoesterase)

PumpkinSpice_236

PumpkinSpice_237

PumpkinSpice_238
(WhiB family transcription factor)

PumpkinSpice_239

PumpkinSpice_240

PumpkinSpice_241

PumpkinSpice_242

PumpkinSpice_243

PumpkinSpice_244

PumpkinSpice_245

PumpkinSpice_246

PumpkinSpice_247

PumpkinSpice_248 — tRNA
(tRNA-Gln(ttg))

PumpkinSpice_249

PumpkinSpice_250

PumpkinSpice_251

PumpkinSpice_252

PumpkinSpice_253

PumpkinSpice_254

PumpkinSpice_255
(thioredoxin)

PumpkinSpice_256

PumpkinSpice_257

PumpkinSpice_258

PumpkinSpice_259

PumpkinSpice_260

PumpkinSpice_261

PumpkinSpice_262

PumpkinSpice_263
(HNH endonuclease)

PumpkinSpice_264

PumpkinSpice_265

PumpkinSpice_266

PumpkinSpice_267

PumpkinSpice_268

PumpkinSpice_269

PumpkinSpice_270

PumpkinSpice_271
(Lsr2-like DNA bridging protein)

PumpkinSpice_272

PumpkinSpice_273

PumpkinSpice_274

PumpkinSpice_275

PumpkinSpice_276

PumpkinSpice_277
(ParB-like nuclease domain protein)

PumpkinSpice_278

PumpkinSpice_279

PumpkinSpice_280

PumpkinSpice_281

PumpkinSpice_282

PumpkinSpice_283

PumpkinSpice_284

PumpkinSpice_285

PumpkinSpice_286

PumpkinSpice_287

PumpkinSpice_288

PumpkinSpice_289

PumpkinSpice_290

PumpkinSpice_291
(HNH endonuclease)

Submitted Annotation in DNA Master FormatDownload
Submitted Minimal DNA Master FileDownload
Author ListDownload
Annotation Cover SheetDownload
Publication Info
Uploaded to GenBank?Yes
GenBank AccessionOQ709199
Refseq NumberNone yet
Archiving Info
Archiving status Archived
SEA Lysate Titer 1.45x10^10
Pitt Freezer Box# 49
Pitt Freezer Box Grid# D2
Available Files
GenBank File for PhameratorDownload