Streptomyces phage PumpkinSpice
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Skelbel (AZ1) Keough (EK2) MamaT (GI) Lilo27 (EB) Liberone (GI)Streptomyces phage PumpkinSpice
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| Detailed Information for Phage PumpkinSpice | |
| Discovery Information | |
| Isolation Host | Streptomyces griseofuscus ATCC 23916 |
| Found By | Brandon Chia & Paul Rice |
| Year Found | 2017 |
| Location Found | St. Louis, MO USA |
| Finding Institution | Washington University in St. Louis |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | No |
| Isolation Temperature | 34°C |
| GPS Coordinates | 38.6457 N, 90.3148 W Map |
| Discovery Notes | Obtained from underneath a tree outside of the Nathan Dardick House. Taken from surface level, <1cm, with an ambient temperature of 93 degrees Fahrenheit |
| Naming Notes | It's fall. |
| Sequencing Information | |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Feb 13, 2018 |
| Sequencing Facility | The McDonnell Genome Institute at Washington University |
| Shotgun Sequencing Method | Illumina |
| Sequencer Used | Illumina MiSeq |
| Read Type | Single-end reads |
| Read Length | 150 bp |
| Approximate Shotgun Coverage | 85 |
| Genome length (bp) | 132480 |
| Character of genome ends | Direct Terminal Repeat |
| Direct Terminal Repeat Length | 12588 bp |
| GC Content | 49.4% |
| Fasta file available? | Yes: Download fasta file |
| Characterization | |
| Cluster | BE |
| Subcluster | BE2 |
| Cluster Life Cycle | Lytic |
| Other Cluster Members | |
| Annotating Institution | University of California, Los Angeles |
| Annotation Status | In GenBank |
| Plaque Notes | The plaque formed were of varying size although overall small. Plaques were clear and had relatively defined edges. |
| Morphotype | Siphoviridae |
| Number of Genes | 246 |
| Number of tRNAs | 44 |
| Number of tmRNAs | 1 |
| Has been Phamerated? | Yes |
| Gene List | |
| Submitted Annotation in DNA Master Format | Download |
| Submitted Minimal DNA Master File | Download |
| Author List | Download |
| Annotation Cover Sheet | Download |
| Publication Info | |
| Uploaded to GenBank? | Yes |
| GenBank Accession | OQ709199 |
| Refseq Number | None yet |
| Archiving Info | |
| Archiving status | Archived |
| SEA Lysate Titer | 1.45x10^10 |
| Pitt Freezer Box# | 49 |
| Pitt Freezer Box Grid# | D2 |
| Available Files | |
| GenBank File for Phamerator | Download |