| Detailed Information for Phage Quaran19 |
| Discovery Information |
| Isolation Host | Streptomyces griseus ATCC 10137 |
| Found By | Evan Barr and Sarah Andersen |
| Year Found | 2020 |
| Location Found | Evansville, IN United States |
| Finding Institution | University of Evansville |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | 28°C |
| GPS Coordinates | 37.974363 N, 87.528507 W Map |
| Discovery Notes | The soil contained mulch and was more of a sand/silt consistency |
| Naming Notes | Since 2020 happened, we decided to name it a mix of quarantine and COVID 19 by creating Quaran19. It's a play on words and a good length so we thought it was clever. |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Jan 6, 2021 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina |
| Sequencer Used | Illumina MiSeq |
| Read Type | Single-end reads |
| Read Length | 150 bp |
| Approximate Shotgun Coverage | 738 |
| Genome length (bp) | 131761 |
| Character of genome ends | Direct Terminal Repeat |
| Overhang Length | 12579 bases |
| GC Content | 49.5% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | BE |
| Subcluster | BE2 |
| Cluster Life Cycle | Lytic |
| Other Cluster Members |
|
| Annotating Institution | University of Evansville |
| Annotation Status | In GenBank |
| Plaque Notes | Clear plaques. They didn't have super clean edges at first, but after many rounds of lysing we started to get clear plaques without halos. |
| Number of Genes | 243 |
| Number of tRNAs | 43 |
| Number of tmRNAs | 1 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Annotation in DNA Master Format | Download |
| Submitted Minimal DNA Master File | Download |
| Author List | Download |
| Annotation Cover Sheet | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | ON260828 |
| Refseq Number | None yet |
| Archiving Info |
| Archiving status |
Archived |
| SEA Lysate Titer |
1.9x10^10 |
| Pitt Freezer Box# |
119 |
| Pitt Freezer Box Grid# |
H9 |
| Available Files |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| EM Picture | Download |
| GenBank File for Phamerator | Download |