Streptomyces phage Shuckle
Shuckle EM Picture
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Detailed Information for Phage Shuckle
Discovery Information
Isolation HostStreptomyces lividans JI 1326
Found ByZechariah Pressnell
Year Found2024
Location FoundSt. Louis, MO United States
Finding InstitutionWashington University in St. Louis
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?Yes
Isolation Temperature19°C
GPS Coordinates38.646681 N, 90.308975 W Map
Discovery NotesSoil texture was dry, loose clay texture, and had little organic matter upon collection. The soil sample was one of 7 taken across an area of a few miles. The sample was enriched before plating.
Naming NotesShuckle, a red and yellow tortoise-like Pokemon, has circular holes in his shell resembling the plaques created by phage Shuckle.
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedJan 17, 2025
Sequencing FacilityThe McDonnell Genome Institute at Washington University
Shotgun Sequencing MethodIllumina
Sequencer UsedIllumina MiSeq
Read TypeSingle-end reads
Read Length150 bp
Approximate Shotgun Coverage184
Genome length (bp)133857
Character of genome endsDirect Terminal Repeat
Direct Terminal Repeat Length11224 bp
GC Content49.6%
Fasta file available?Yes: Download fasta file
Characterization
ClusterBE
SubclusterBE1
Cluster Life CycleLytic
Other Cluster Members
Annotating InstitutionWashington University in St. Louis
Annotation StatusIn GenBank
Plaque NotesPlaques were consistently clear, regular, and circular with defined edges. The diameter was medium in size, about 2.91mm on average.
Number of Genes227
Number of tRNAs42
Number of tmRNAs1
Has been Phamerated?Yes
Gene List

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Shuckle_1

Shuckle_2

Shuckle_3
(ribosomal protein, bL12)

Shuckle_4
(ribosomal protein, bL12)

Shuckle_5

Shuckle_6

Shuckle_7
(Lsr2-like DNA bridging protein)

Shuckle_8

Shuckle_9

Shuckle_10
(ParB N-terminal-like domain)

Shuckle_11
(ParB N-terminal-like domain)

Shuckle_12

Shuckle_13

Shuckle_14

Shuckle_15

Shuckle_16

Shuckle_17

Shuckle_18

Shuckle_19

Shuckle_20

Shuckle_21

Shuckle_22
(HNH endonuclease)

Shuckle_23

Shuckle_24
(ThyX-like thymidylate synthase)

Shuckle_25
(LAGLIDADG endonuclease)

Shuckle_26

Shuckle_27

Shuckle_28

Shuckle_29

Shuckle_30

Shuckle_31

Shuckle_32

Shuckle_33
(nucleotidyl transferase)

Shuckle_34
(glycosyltransferase)

Shuckle_35
(glycosyltransferase)

Shuckle_36 — tRNA
(tRNA-Thr(cgt))

Shuckle_37
(HNH endonuclease)

Shuckle_38
(glycosyltransferase)

Shuckle_39

Shuckle_40
(glycosyltransferase)

Shuckle_41
(ribonucleotide reductase)

Shuckle_42
(endolysin)

Shuckle_43

Shuckle_44

Shuckle_45
(portal protein)

Shuckle_46
(holin)

Shuckle_47
(capsid maturation protease)

Shuckle_48
(major capsid protein)

Shuckle_49

Shuckle_50
(head-to-tail adaptor)

Shuckle_51
(head-to-tail stopper)

Shuckle_52

Shuckle_53
(tail terminator)

Shuckle_54
(major tail protein)

Shuckle_55
(tail assembly chaperone)

Shuckle_56

Shuckle_57
(tape measure protein)

Shuckle_58
(minor tail protein)

Shuckle_59
(minor tail protein)

Shuckle_60

Shuckle_61
(minor tail protein)

Shuckle_62
(minor tail protein)

Shuckle_63

Shuckle_64

Shuckle_65

Shuckle_66
(HNH endonuclease)

Shuckle_67
(metallophosphatase)

Shuckle_68

Shuckle_69

Shuckle_70
(helix-turn-helix DNA binding domain)

Shuckle_71

Shuckle_72

Shuckle_73

Shuckle_74
(DnaB-like dsDNA helicase)

Shuckle_75

Shuckle_76
(DNA primase)

Shuckle_77
(SSB protein)

Shuckle_78
(DnaE-like DNA polymerase III (alpha))

Shuckle_79

Shuckle_80
(MazG-like nucleotide pyrophosphohydrolase)

Shuckle_81
(RecA-like DNA recombinase)

Shuckle_82

Shuckle_83
(Holliday junction resolvase)

Shuckle_84

Shuckle_85

Shuckle_86
(Cas4 family exonuclease)

Shuckle_87

Shuckle_88
(RuvC-like resolvase)

Shuckle_89
(DprA-like DNA processing chain A)

Shuckle_90
(helix-turn-helix DNA binding domain)

Shuckle_91
(thymidylate kinase)

Shuckle_92

Shuckle_93
(terminase, small subunit)

Shuckle_94
(terminase, large subunit)

Shuckle_95
(HNH endonuclease)

Shuckle_96

Shuckle_97 — tRNA
(tRNA-Gly(tcc))

Shuckle_98 — tRNA
(tRNA-Gln(ctg))

Shuckle_99 — tRNA
(tRNA-Trp(cca))

Shuckle_100

Shuckle_101

Shuckle_102 — tRNA
(tRNA-Pro(tgg))

Shuckle_103 — tRNA
(tRNA-Pro(tgg))

Shuckle_104
(nucleotidyl transferase)

Shuckle_105
(FtsK-like DNA translocase)

Shuckle_106

Shuckle_107 — tRNA
(tRNA-Ile(tat))

Shuckle_108

Shuckle_109

Shuckle_110 — tRNA
(tRNA-Ser(gga))

Shuckle_111

Shuckle_112 — tRNA
(tRNA-Ser(tga))

Shuckle_113

Shuckle_114

Shuckle_115

Shuckle_116 — tRNA
(tRNA-Asp(gtc))

Shuckle_117

Shuckle_118

Shuckle_119

Shuckle_120 — tRNA
(tRNA-Asp(gtc))

Shuckle_121

Shuckle_122

Shuckle_123
(Ro-like RNA binding protein)

Shuckle_124

Shuckle_125
(HNH endonuclease)

Shuckle_126

Shuckle_127

Shuckle_128

Shuckle_129

Shuckle_130

Shuckle_131

Shuckle_132 — tRNA
(tRNA-Glu(ctc))

Shuckle_133 — tRNA
(tRNA-Glu(ttc))

Shuckle_134

Shuckle_135 — tRNA
(tRNA-Val(tac))

Shuckle_136 — tRNA
(tRNA-Leu(tag))

Shuckle_137 — tRNA
(tRNA-Leu(tag))

Shuckle_138 — tRNA
(tRNA-Gly(gcc))

Shuckle_139 — tRNA
(tRNA-Arg(tct))

Shuckle_140

Shuckle_141

Shuckle_142

Shuckle_143 — tRNA
(tRNA-Ala(tgc))

Shuckle_144 — tRNA
(tRNA-Lys(ctt))

Shuckle_145
(peptidase)

Shuckle_146

Shuckle_147 — tRNA
(tRNA-Asn(gtt))

Shuckle_148

Shuckle_149

Shuckle_150 — tRNA
(tRNA-Asn(gtt))

Shuckle_151
(membrane protein, Band-7-like)

Shuckle_152

Shuckle_153

Shuckle_154 — tRNA
(tRNA-Arg(cct))

Shuckle_155

Shuckle_156 — tRNA
(tRNA-Ile(gat))

Shuckle_157 — tRNA
(tRNA-Ala(ggc))

Shuckle_158 — tRNA
(tRNA-Lys(ctt))

Shuckle_159 — tRNA
(tRNA-Lys(ttt))

Shuckle_160

Shuckle_161 — tRNA
(tRNA-Arg(tcg))

Shuckle_162
(tellurium resistance protein D family)

Shuckle_163 — tRNA
(tRNA-Met(cat))

Shuckle_164 — tRNA
(tRNA-Met(cat))

Shuckle_165

Shuckle_166

Shuckle_167

Shuckle_168 — tRNA
(tRNA-Tyr(gta))

Shuckle_169 — tRNA
(tRNA-Phe(gaa))

Shuckle_170

Shuckle_171

Shuckle_172 — tRNA
(tRNA-Cys(gca))

Shuckle_173 — tRNA
(tRNA-Thr(tgt))

Shuckle_174 — tRNA
(tRNA-Thr(ggt))

Shuckle_175

Shuckle_176 — tRNA
(tRNA-Arg(acg))

Shuckle_177

Shuckle_178
(DnaQ-like (DNA polymerase III subunit))

Shuckle_179 — tmRNA
(Tag peptide: ANSKFAFALAA*)

Shuckle_180

Shuckle_181
(peptidase)

Shuckle_182

Shuckle_183

Shuckle_184
(acetyltransferase)

Shuckle_185

Shuckle_186 — tRNA
(tRNA-Ala(ggc))

Shuckle_187

Shuckle_188

Shuckle_189

Shuckle_190

Shuckle_191
(RNA ligase)

Shuckle_192

Shuckle_193

Shuckle_194

Shuckle_195
(polynucleotide kinase)

Shuckle_196

Shuckle_197

Shuckle_198 — tRNA
(tRNA-Leu(gag))

Shuckle_199

Shuckle_200

Shuckle_201 — tRNA
(tRNA-Val(gac))

Shuckle_202

Shuckle_203 — tRNA
(tRNA-Leu(caa))

Shuckle_204

Shuckle_205 — tRNA
(tRNA-Ser(gct))

Shuckle_206

Shuckle_207

Shuckle_208

Shuckle_209

Shuckle_210

Shuckle_211

Shuckle_212

Shuckle_213

Shuckle_214
(deoxycytidylate deaminase)

Shuckle_215

Shuckle_216
(rIIA-like protein)

Shuckle_217
(rIIB-like protein)

Shuckle_218
(metallophosphatase)

Shuckle_219

Shuckle_220

Shuckle_221
(HNH endonuclease)

Shuckle_222
(WhiB family transcription factor)

Shuckle_223

Shuckle_224

Shuckle_225

Shuckle_226

Shuckle_227

Shuckle_228

Shuckle_229

Shuckle_230

Shuckle_231 — tRNA
(tRNA-Gln(ttg))

Shuckle_232

Shuckle_233

Shuckle_234

Shuckle_235

Shuckle_236

Shuckle_237

Shuckle_238

Shuckle_239

Shuckle_240
(thioredoxin)

Shuckle_241

Shuckle_242

Shuckle_243

Shuckle_244

Shuckle_245

Shuckle_246

Shuckle_247

Shuckle_248

Shuckle_249

Shuckle_250

Shuckle_251
(ribosomal protein, bL12)

Shuckle_252
(ribosomal protein, bL12)

Shuckle_253

Shuckle_254

Shuckle_255
(Lsr2-like DNA bridging protein)

Shuckle_256

Shuckle_257

Shuckle_258
(ParB N-terminal-like domain)

Shuckle_259
(ParB N-terminal-like domain)

Shuckle_260

Shuckle_261

Shuckle_262

Shuckle_263

Shuckle_264

Shuckle_265

Shuckle_266

Shuckle_267

Shuckle_268

Shuckle_269

Shuckle_270
(HNH endonuclease)

Submitted Minimal DNA Master FileDownload
Publication Info
Uploaded to GenBank?Yes
GenBank AccessionPV940984
Refseq NumberNone yet
Archiving Info
Archiving status Not in Pitt Archives
Available Files
Plaque PictureDownload
GenBank File for PhameratorDownload