| Discovery Information |
| Isolation Host | Streptomyces lividans JI 1326 |
| Found By | Zechariah Pressnell |
| Year Found | 2024 |
| Location Found | St. Louis, MO United States |
| Finding Institution | Washington University in St. Louis |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | 19°C |
| GPS Coordinates | 38.646681 N, 90.308975 W Map |
| Discovery Notes | Soil texture was dry, loose clay texture, and had little organic matter upon collection. The soil sample was one of 7 taken across an area of a few miles. The sample was enriched before plating. |
| Naming Notes | Shuckle, a red and yellow tortoise-like Pokemon, has circular holes in his shell resembling the plaques created by phage Shuckle. |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Jan 17, 2025 |
| Sequencing Facility | The McDonnell Genome Institute at Washington University |
| Shotgun Sequencing Method | Illumina |
| Sequencer Used | Illumina MiSeq |
| Read Type | Single-end reads |
| Read Length | 150 bp |
| Approximate Shotgun Coverage | 184 |
| Genome length (bp) | 133857 |
| Character of genome ends | Direct Terminal Repeat |
| Direct Terminal Repeat Length | 11224 bp |
| GC Content | 49.6% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | BE |
| Subcluster | BE1 |
| Cluster Life Cycle | Lytic |
| Other Cluster Members |
|
| Annotating Institution | Washington University in St. Louis |
| Annotation Status | In GenBank |
| Plaque Notes | Plaques were consistently clear, regular, and circular with defined edges. The diameter was medium in size, about 2.91mm on average. |
| Number of Genes | 227 |
| Number of tRNAs | 42 |
| Number of tmRNAs | 1 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Minimal DNA Master File | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | PV940984 |
| Refseq Number | None yet |
| Archiving Info |
| Archiving status |
Not in Pitt Archives |
| Available Files |
| Plaque Picture | Download |
| GenBank File for Phamerator | Download |
