| Detailed Information for Phage Spilled |
| Discovery Information |
| Isolation Host | Streptomyces griseus ATCC 10137 |
| Found By | Xavier Kujawa, Lillie Oelker, Nick B, Connor Y |
| Year Found | 2021 |
| Location Found | Evansville, IN United States of America |
| Finding Institution | University of Evansville |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | Not entered |
| GPS Coordinates | 37.972 N, 87.533 W Map |
| Discovery Notes | The texture of the soil was dry, clay-like, and composed of chunks rather than particles |
| Naming Notes | Named Spilled because the phage buffer and bacterial cells were accidentally spilled during the experiment. |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Jan 10, 2022 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina |
| Sequencer Used | Illumina MiSeq |
| Read Type | Single-end reads |
| Read Length | 150 bp |
| Approximate Shotgun Coverage | 1248 |
| Genome length (bp) | 132667 |
| Character of genome ends | Direct Terminal Repeat |
| Direct Terminal Repeat Length | 12198 bp |
| GC Content | 49.3% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | BE |
| Subcluster | BE2 |
| Cluster Life Cycle | Lytic |
| Other Cluster Members |
|
| Annotating Institution | University of Evansville |
| Annotation Status | In GenBank |
| Plaque Notes | Larger plaques |
| Number of Genes | 249 |
| Number of tRNAs | 44 |
| Number of tmRNAs | 1 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Annotation in DNA Master Format | Download |
| Submitted Minimal DNA Master File | Download |
| Author List | Download |
| Annotation Cover Sheet | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | ON970579 |
| Refseq Number | None yet |
| Archiving Info |
| Archiving status |
Archived |
| SEA Lysate Titer |
1.16x10^10 pfu/ml |
| Pitt Freezer Box# |
137 |
| Pitt Freezer Box Grid# |
F4 |
| Available Files |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| EM Picture | Download |
| GenBank File for Phamerator | Download |
