Streptomyces phage Spilled
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Detailed Information for Phage Spilled
Discovery Information
Isolation HostStreptomyces griseus ATCC 10137
Found ByXavier Kujawa, Lillie Oelker, Nick B, Connor Y
Year Found2021
Location FoundEvansville, IN United States of America
Finding InstitutionUniversity of Evansville
ProgramScience Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science
From enriched soil sample?Yes
Isolation TemperatureNot entered
GPS Coordinates37.972 N, 87.533 W Map
Discovery NotesThe texture of the soil was dry, clay-like, and composed of chunks rather than particles
Naming NotesNamed Spilled because the phage buffer and bacterial cells were accidentally spilled during the experiment.
Sequencing Information
Sequencing Complete?Yes
Date Sequencing CompletedJan 10, 2022
Sequencing FacilityPittsburgh Bacteriophage Institute
Shotgun Sequencing MethodIllumina Sequencing
Approximate Shotgun Coverage1248
Genome length (bp)132667
Character of genome endsDirect Terminal Repeat
Direct Terminal Repeat Length12198 bp
GC Content49.3%
Fasta file available?Yes: Download fasta file
Characterization
ClusterBE
SubclusterBE2
Cluster Life CycleLytic
Other Cluster Members
Annotating InstitutionUniversity of Evansville
Annotation StatusIn GenBank
Plaque NotesLarger plaques
Number of Genes249
Number of tRNAs44
Number of tmRNAs1
Has been Phamerated?Yes
Gene List

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Spilled_1

Spilled_2

Spilled_3

Spilled_4

Spilled_5

Spilled_6
(Lsr2-like DNA binding protein)

Spilled_7

Spilled_8

Spilled_9

Spilled_10

Spilled_11

Spilled_12
(ParB-like nuclease domain protein)

Spilled_13

Spilled_14

Spilled_15

Spilled_16

Spilled_17

Spilled_18

Spilled_19

Spilled_20

Spilled_21

Spilled_22

Spilled_23

Spilled_24

Spilled_25

Spilled_26
(HNH endonuclease)

Spilled_27

Spilled_28

Spilled_29
(ThyX-like thymidylate synthase)

Spilled_30

Spilled_31

Spilled_32

Spilled_33

Spilled_34

Spilled_35

Spilled_36

Spilled_37

Spilled_38

Spilled_39 — tRNA
(tRNA-Thr(cgt))

Spilled_40
(HNH endonuclease)

Spilled_41
(endolysin)

Spilled_42

Spilled_43

Spilled_44
(glycosyltransferase)

Spilled_45
(ribonucleotide reductase)

Spilled_46
(endolysin, N-acetylmuramoyl-L-alanine amidase domain)

Spilled_47
(HNH endonuclease)

Spilled_48

Spilled_49

Spilled_50
(portal protein)

Spilled_51

Spilled_52
(capsid maturation protease)

Spilled_53
(major capsid protein)

Spilled_54

Spilled_55
(head-to-tail adaptor)

Spilled_56
(head-to-tail stopper)

Spilled_57

Spilled_58

Spilled_59
(major tail protein)

Spilled_60
(tail assembly chaperone)

Spilled_61
(tail assembly chaperone)

Spilled_62
(tape measure protein)

Spilled_63

Spilled_64
(minor tail protein)

Spilled_65

Spilled_66
(minor tail protein)

Spilled_67
(minor tail protein)

Spilled_68

Spilled_69

Spilled_70

Spilled_71
(HNH endonuclease)

Spilled_72
(metallophosphoesterase)

Spilled_73
(holin)

Spilled_74
(helix-turn-helix DNA binding domain protein)

Spilled_75
(helix-turn-helix DNA binding domain protein)

Spilled_76

Spilled_77

Spilled_78

Spilled_79
(DnaB-like dsDNA helicase)

Spilled_80

Spilled_81
(DNA primase)

Spilled_82
(ssDNA binding protein)

Spilled_83
(DnaE-like DNA polymerase III alpha)

Spilled_84

Spilled_85
(MazG-like nucleotide pyrophosphohydrolase)

Spilled_86
(RecA-like DNA recombinase)

Spilled_87

Spilled_88
(holliday junction resolvase)

Spilled_89

Spilled_90

Spilled_91

Spilled_92
(Cas4 family exonuclease)

Spilled_93

Spilled_94
(RuvC-like resolvase)

Spilled_95
(DprA-like ssDNA binding protein)

Spilled_96
(helix-turn-helix DNA binding domain protein)

Spilled_97
(methyltransferase)

Spilled_98
(thymidylate kinase)

Spilled_99

Spilled_100
(DNA binding domain protein)

Spilled_101
(terminase)

Spilled_102
(HNH endonuclease)

Spilled_103

Spilled_104 — tRNA
(tRNA-Gly(tcc))

Spilled_105 — tRNA
(tRNA-Gln(ctg))

Spilled_106 — tRNA
(tRNA-Trp(cca))

Spilled_107

Spilled_108

Spilled_109 — tRNA
(tRNA-Pro(tgg))

Spilled_110 — tRNA
(tRNA-Pro(tgg))

Spilled_111
(nucleotidyltransferase)

Spilled_112
(FtsK-like DNA translocase)

Spilled_113

Spilled_114 — tRNA
(tRNA-Ile(tat))

Spilled_115

Spilled_116 — tRNA
(tRNA-Leu(taa))

Spilled_117

Spilled_118

Spilled_119 — tRNA
(tRNA-Ser(gga))

Spilled_120

Spilled_121 — tRNA
(tRNA-Ser(tga))

Spilled_122

Spilled_123

Spilled_124

Spilled_125

Spilled_126

Spilled_127

Spilled_128 — tRNA
(tRNA-Asp(gtc))

Spilled_129

Spilled_130

Spilled_131 — tRNA
(tRNA-Asp(gtc))

Spilled_132

Spilled_133
(Ro-like RNA binding protein)

Spilled_134

Spilled_135

Spilled_136

Spilled_137

Spilled_138
(DNA binding protein)

Spilled_139

Spilled_140

Spilled_141

Spilled_142

Spilled_143

Spilled_144 — tRNA
(tRNA-Glu(ctc))

Spilled_145

Spilled_146 — tRNA
(tRNA-Glu(ttc))

Spilled_147 — tRNA
(tRNA-Val(tac))

Spilled_148 — tRNA
(tRNA-Leu(tag))

Spilled_149 — tRNA
(tRNA-Leu(tag))

Spilled_150 — tRNA
(tRNA-Phe(gaa))

Spilled_151
(HNH endonuclease)

Spilled_152 — tRNA
(tRNA-Gly(gcc))

Spilled_153 — tRNA
(tRNA-Arg(tct))

Spilled_154

Spilled_155

Spilled_156

Spilled_157 — tRNA
(tRNA-Ala(tgc))

Spilled_158 — tRNA
(tRNA-Lys(ctt))

Spilled_159
(peptidase)

Spilled_160

Spilled_161 — tRNA
(tRNA-Asn(gtt))

Spilled_162

Spilled_163

Spilled_164

Spilled_165

Spilled_166 — tRNA
(tRNA-Asn(gtt))

Spilled_167
(band-7-like membrane protein)

Spilled_168

Spilled_169

Spilled_170 — tRNA
(tRNA-Arg(cct))

Spilled_171

Spilled_172 — tRNA
(tRNA-Ala(ggc))

Spilled_173 — tRNA
(tRNA-Lys(ctt))

Spilled_174 — tRNA
(tRNA-Lys(ctt))

Spilled_175

Spilled_176 — tRNA
(tRNA-Lys(ttt))

Spilled_177

Spilled_178 — tRNA
(tRNA-Arg(tcg))

Spilled_179

Spilled_180 — tRNA
(tRNA-Met(cat))

Spilled_181 — tRNA
(tRNA-Met(cat))

Spilled_182

Spilled_183

Spilled_184 — tRNA
(tRNA-Tyr(gta))

Spilled_185 — tRNA
(tRNA-His(gtg))

Spilled_186

Spilled_187

Spilled_188

Spilled_189 — tRNA
(tRNA-cys(gca))

Spilled_190 — tRNA
(tRNA-Thr(tgt))

Spilled_191 — tRNA
(tRNA-Thr(ggt))

Spilled_192

Spilled_193 — tRNA
(tRNA-Arg(acg))

Spilled_194
(HNH endonuclease)

Spilled_195

Spilled_196
(DnaQ-like exonuclease)

Spilled_197

Spilled_198 — tmRNA
(Tag peptide: ANSNVASAYALAA*)

Spilled_199

Spilled_200

Spilled_201
(acetyltransferase)

Spilled_202

Spilled_203

Spilled_204 — tRNA
(tRNA-?(Ala|Gly)(gc))

Spilled_205

Spilled_206

Spilled_207

Spilled_208

Spilled_209

Spilled_210
(RNA ligase)

Spilled_211

Spilled_212

Spilled_213

Spilled_214

Spilled_215
(polynucleotide kinase)

Spilled_216

Spilled_217

Spilled_218

Spilled_219 — tRNA
(tRNA-Leu(gag))

Spilled_220

Spilled_221

Spilled_222 — tRNA
(tRNA-Val(gac))

Spilled_223
(SprT-like protease)

Spilled_224

Spilled_225

Spilled_226

Spilled_227 — tRNA
(tRNA-Leu(caa))

Spilled_228

Spilled_229 — tRNA
(tRNA-Ser(gct))

Spilled_230

Spilled_231

Spilled_232

Spilled_233

Spilled_234

Spilled_235

Spilled_236

Spilled_237

Spilled_238
(deoxycytidylate deaminase)

Spilled_239
(phosphoesterase)

Spilled_240

Spilled_241

Spilled_242
(WhiB family transcription factor)

Spilled_243

Spilled_244

Spilled_245

Spilled_246

Spilled_247

Spilled_248

Spilled_249

Spilled_250

Spilled_251

Spilled_252 — tRNA
(tRNA-Gln(ttg))

Spilled_253

Spilled_254

Spilled_255

Spilled_256

Spilled_257

Spilled_258

Spilled_259
(HNH endonuclease)

Spilled_260
(thioredoxin)

Spilled_261

Spilled_262

Spilled_263

Spilled_264

Spilled_265

Spilled_266

Spilled_267

Spilled_268
(endonuclease VII)

Spilled_269

Spilled_270

Spilled_271

Spilled_272

Spilled_273

Spilled_274
(Lsr2-like DNA bridging protein)

Spilled_275

Spilled_276

Spilled_277

Spilled_278

Spilled_279

Spilled_280
(ParB-like nuclease domain protein)

Spilled_281

Spilled_282

Spilled_283

Spilled_284

Spilled_285

Spilled_286

Spilled_287

Spilled_288

Spilled_289

Spilled_290

Spilled_291

Spilled_292

Spilled_293

Spilled_294
(HNH endonuclease)

Submitted Annotation in DNA Master FormatDownload
Submitted Minimal DNA Master FileDownload
Author ListDownload
Annotation Cover SheetDownload
Publication Info
Uploaded to GenBank?Yes
GenBank AccessionON970579
Refseq NumberNone yet
Archiving Info
Archiving status Archived
SEA Lysate Titer 1.16x10^10 pfu/ml
Pitt Freezer Box# 137
Pitt Freezer Box Grid# F4
Available Files
Plaque PictureDownload
Restriction Digest PictureDownload
EM PictureDownload
GenBank File for PhameratorDownload