| Detailed Information for Phage Tomas |
| Discovery Information |
| Isolation Host | Streptomyces sanglieri UNT16F27A |
| Found By | Shelby Harris |
| Year Found | 2020 |
| Location Found | Lake Worth, TX United States |
| Finding Institution | University of North Texas |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | 30°C |
| GPS Coordinates | 32.821 N, 97.429 W Map |
| Discovery Notes | This soil sample was collected January 12, 2020. The soil was moist. It was collected at 2:17pm. the weather was sunny but still chilly with a temperature of 64˚C. The address where it was collected was 4504 Norris Valley Dr. Lake Worth Texas. There was little grass and much soil and it was near a sprinkler system as well. |
| Naming Notes | I named my soil sample Tomas because that is my grandfathers name. He was the one who pushed me to pursue higher level education because he could never complete his education. His aspiration for me was to become a doctor. He passed away my junior year of high school. I wanted to name my sample after him because I'm here doing this research because of him. |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Aug 7, 2020 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina Sequencing |
| Approximate Shotgun Coverage | 486 |
| Genome length (bp) | 134464 |
| Character of genome ends | Direct Terminal Repeat |
| Direct Terminal Repeat Length | 12757 bp |
| GC Content | 48.2% |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | BE |
| Subcluster | BE2 |
| Cluster Life Cycle | Lytic |
| Lysogeny Notes | I believe my phage was a lytic phage. It was always clear, never cloudy, and there were no mesas. |
| Other Cluster Members |
|
| Annotating Institution | University of North Texas |
| Annotation Status | In GenBank |
| Plaque Notes | The plaques were about 0.5mm in diameter. They were round and clear in every experiment. |
| Number of Genes | 237 |
| Number of tRNAs | 45 |
| Number of tmRNAs | 1 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Minimal DNA Master File | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | OL829978 |
| Refseq Number | None yet |
| Archiving Info |
| Archiving status |
Archived |
| Pitt Freezer Box# |
118 |
| Pitt Freezer Box Grid# |
B1 |
| Available Files |
| Plaque Picture | Download |
| Restriction Digest Picture | Download |
| GenBank File for Phamerator | Download |
