| Discovery Information |
| Isolation Host | Microbacterium foliorum NRRL B-24224 |
| Found By | Miguel Reyes Serrano |
| Year Found | 2024 |
| Location Found | Las Piedras, PR United States |
| Finding Institution | Universidad Ana G. Mendez, Gurabo campus |
| Program | Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science |
| From enriched soil sample? | Yes |
| Isolation Temperature | 30°C |
| GPS Coordinates | 18.16301 N, 65.87964 W Map |
| Discovery Notes | When I was collecting the sample, it was raining. The soil is used for a garden, the color is dark brown, lumpy, was a little wet, has small trees around and a little of grass. The temperature was around 76º F and the humidity was 84%. |
| Naming Notes | The name of this phage is my nickname, my family always calls me Tongui. |
| Sequencing Information |
| Sequencing Complete? | Yes |
| Date Sequencing Completed | Jan 27, 2025 |
| Sequencing Facility | Pittsburgh Bacteriophage Institute |
| Shotgun Sequencing Method | Illumina |
| Sequencer Used | Illumina NextSeq 1000 |
| Read Type | Single-end reads |
| Read Length | 100 bp |
| Approximate Shotgun Coverage | 2282 |
| Genome length (bp) | 56212 |
| Character of genome ends | Circularly Permuted |
| GC Content | 64.0% |
| Sequencing Notes | ~40% reads contain a 42 bp deletion of 19648-19689 bp |
| Fasta file available? | Yes: Download fasta file |
| Characterization |
| Cluster | EF |
| Subcluster | -- |
| Cluster Life Cycle | Lytic |
| Other Cluster Members |
|
| Annotating Institution | Universidad Ana G. Mendez, Gurabo campus |
| Annotation Status | In GenBank |
| Plaque Notes | The plaque morphology are small, has a little dark dot in the center of every plaque and they are a little turbid. |
| Morphotype | Siphoviridae |
| Number of Genes | 83 |
| Number of tRNAs | 0 |
| Number of tmRNAs | 0 |
| Has been Phamerated? | Yes |
| Gene List |
|
| Submitted Annotation in DNA Master Format | Download |
| Submitted Minimal DNA Master File | Download |
| Author List | Download |
| Annotation Cover Sheet | Download |
| Publication Info |
| Uploaded to GenBank? | Yes |
| GenBank Accession | PV915848 |
| Refseq Number | None yet |
| Archiving Info |
| Archiving status |
Archived |
| Pitt Freezer Box# |
197 |
| Pitt Freezer Box Grid# |
H8 |
| Available Files |
| Plaque Picture | Download |
| GenBank File for Phamerator | Download |
